NOS (pan) Antibody 2977S from Cell Signaling Technology

Product Specs
ItemNOS (pan) Antibody
CompanyCell Signaling Technology
Price $306.00 Inquire
Catalog Number2977S
Quantity100 µl
ApplicationsW
ReactivityH, M
TargetiNOS mouse
HostRabbit
NCBI Gene AliasesMAC-NOS, NOS-II, Nos-2, Nos2a, i-NOS, iNOS
NCBI Full Gene Namenitric oxide synthase 2, inducible
ClonalityPolyclonal
Research AreaImmunology and Inflammation
Molecular Weight130, 140, 160

Questions and Answers

Can this NOS (pan) antibody be used to study the effects of IL-17 on NOS2 expression in macrophages?

Yes, the NOS (pan) antibody has been utilized in studies examining the regulation of NOS2 expression in RAW 264.7 cells, particularly in the context of IL-17 and IFN-γ co-treatment, which intensifies NOS2 upregulation and nitric oxide production. This suggests its applicability in investigating inflammatory signaling pathways involving NOS2 in macrophages.

Is this antibody effective for detecting inducible nitric oxide synthase in Western blotting?

The NOS (pan) antibody has been cited in multiple studies for its effectiveness in Western blotting to detect inducible nitric oxide synthase (iNOS). For instance, it has been used to assess iNOS expression in various experimental setups, including those involving lipopolysaccharide-stimulated microglial cells.

What species can this antibody detect, and is it suitable for use in human studies?

The NOS (pan) antibody is reactive with both mouse and human proteins, making it suitable for studies involving these species. Its application in human research has been documented, indicating its relevance for investigations into human inflammatory responses.

Has this antibody been used in studies related to neuroinflammation?

Yes, the NOS (pan) antibody has been referenced in studies focusing on neuroinflammation, particularly in the context of microglial activation and the production of pro-inflammatory cytokines. It has been employed to analyze the expression of iNOS in microglial cells treated with inflammatory stimuli, highlighting its role in neuroinflammatory research.

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(1) LIF and Oncostatin M mitigate LPS-induced acute endometritis via distinct immunoregulatory mechanisms in peritoneal macrophages in vivoiScienceJune 19, 2026Marion Ravelojaona, Julie Girouard, Cathy Vaillancourt, Céline Van Themsche, Carlos Reyes-Morenokit,"FroggaBio (Concord, ON, Canada)",#CCH365 iNOS,"Cell Signaling Technologies (Danvers, MA, USA)",#2977 Arginase-1 (Arg-1),"Cell Signaling Technologies (Danvers, MA, USA)",#93668 phospho-STAT3 (pY705),"Cell

(2) Nitric Oxide, Reactive Oxygen Species, and Focal Adhesion Kinase Mediate Anoikis Resistance in A375 and SK-MEL-28 Human Melanoma CellsAntioxidants (Basel)June 10, 2026Igor R do do Nascimento, Ana Caroline S. Teodoro, Paulo V de de Sousa, Leticia T. Barboza, Joanderson P. Cândido da Silva, Ricardo C. Cintra, Caroline Alves, Lidia R. De Toledo, Ronaldo J. Carneiro, Luiz S. Longo, et al.rabbit anti-Alpha-tubulin (1:1000—Cell Signaling, 2144S); rabbit anti-pan-NOS (1:1000—Cell Signaling, 2977); rabbit anti-GAPDH (1:1000—Cell Signaling, 4874); rabbit anti-Phospho-FAK (Y397) (1:1000—Millipore,

(3) Antioxidant and Anti-Inflammatory Activities of Cutlassfish Head Peptone in RAW 264.7 MacrophagesAntioxidants (Basel)February 27, 2025Su-Jin Lee, Jeonghyeon Im, Svini Dileepa Marasinghe, Eunyoung Jo, Minthari Sakethanika Bandara, Youngdeuk Lee, Jaewon Lee, Gun-Hoo Park, Chulhong Oh4 °C with primary antibodies, including iNOS (Cell Signaling Technology, Inc. (Danvers, MA, USA), #2977, 1:1000), COX-2 (Cell Signaling, #4842, 1:1000), Phospho-p38 (Cell Signaling, #4511, 1:500), p38 (Cell Signaling

(4) Ampelopsis japonica enhances the effect of radiotherapy in non-small cell lung cancerStrahlentherapie und OnkologieDecember 4, 2024Zhaohua Liu, Peixia Cui, Qian Wu, Xiao Ji#9272, Cell Signaling Technology), AKT (1:1000, #9271, Cell Signaling Technology), p‑NOS (1:1000, #2977, Cell Signaling Technology), NOS (1:1000, #9575, Cell Signaling Technology), and GAPDH (1:1000, ab9485,

(5) Inhibitory Effects of New Epicatechin Oligomers on Nitric Oxide ProductionInternational Journal of Molecular SciencesOctober 14, 2024Gyeong Han Jeong, Hanui Lee, Byung Yeoup Chung, Hyoung-Woo Baitemperature. They were then incubated overnight at 4 °C with primary antibodies against iNOS (cat. #2977) and GAPDH (cat. #2118), both diluted at 1:1000 (antibodies from Cell Signaling Technology, Danvers,

(6) Ionizing radiation‑induced modification of nialamide as an anti‑inflammatory agent against lipopolysaccharide‑induced RAW 264.7 and DH82 cellsExperimental and therapeutic medicineMay 1, 2024Lee H, Jeong GH, Woo SY, Choi HJ, Chung BY, Lee KB, Bai HW.following primary antibodies at dilution of 1:1,000: Anti-COX-2 (cat. no. 4842), anti-iNOS (cat. no. 2977), anti-p65 (cat. no. 8242), anti-p-p65 (cat. no. 3033), anti-IκB (cat. no. 4812), anti-p-IκB (cat. no.

(7) Targeting ALK averts ribonuclease 1-induced immunosuppression and enhances antitumor immunity in hepatocellular carcinomaNature communicationsFebruary 2, 2024Liu C, Zhou C, Xia W, Zhou Y, Qiu Y, Weng J, Zhou Q, Chen W, Wang YN, Lee HH, et al.(Polyclonal, cat. no. 9102; Cell Signaling Technology; 1:3000), and rabbit anti-iNOS (Polyclonal, cat. no. 2977; Cell Signaling Technology; 1:1000); and rabbit anti-CD206 (Polyclonal, cat. no. NBP1-90020; Novus Biologicals;

(8) Comparing associations of handgrip strength and chair stand performance with all-cause mortality—implications for defining probable sarcopenia: the Tromsø Study 2015–2020BMC MedicineNovember 20, 2023Jonas Johansson, Sameline Grimsgaard, Bjørn Heine Strand, Avan A. Sayer, Rachel Cooper(s),5-CST performance (s),5-CST performance (s),5-CST performance (s) T-score < 1 (not low),< 11.1,2977,69.6,,< 10.4,2615,73.3, T-score 1 to 2.49 (low),11.1–15.9,1063,24.9,,10.4–14.6,779,21.9, T-score ≥

(9) Novel aminopyridazine derivative of minaprine modified by radiolysis presents potent anti-inflammatory effects in LPS-stimulated RAW 264.7 and DH82 macrophage cellsScientific reportsJuly 5, 2023Jeong GH, Lee H, Woo SY, Lee HK, Chung BY, Bai HW.at 4 °C with primary antibodies at dilution of 1:1,000 for anti-COX-2 (cat. #4842), anti-iNOS (cat. #2977), and anti‑GAPDH (cat. no. 2118) (all primary antibodies; Cell Signaling Technology, Danvers, MA, USA).

(10) Mild treadmill exercise inhibits cartilage degeneration via macrophages in an osteoarthritis mouse modelOsteoarthritis and cartilage openJune 1, 2023Oka Y, Murata K, Ozone K, Minegishi Y, Kano T, Shimada N, Kanemura N.Bioss Antibodies Co., Ltd. MA, USA, 1:100), anti-CD68 (ab125212; Abcam plc. MA, USA, 1:100), anti-iNOS (2977S; Cell Signaling Technology, Inc., MA, USA, 1:200), anti-CD206 (18704-1-AP; Proteintech Group, IL, USA,

(11) Infiltration of Tumors Is Regulated by T cell-Intrinsic Nitric Oxide SynthesisCancer immunology researchMarch 1, 2023Cunha PP, Bargiela D, Minogue E, Krause LCM, Barbieri L, Brombach C, Gojkovic M, Marklund E, Pietsch S, Foskolou I, et al.solution. Mouse protein extracts (25–40 μg) were probed with primary antibodies against panNOS (CST #2977), NOS2 (CST #13120), and hypoxia-inducible factor (HIF)-1α (Novus NB-100–449) and detected using infrared-labeled

(12) The E3 ubiquitin ligase RNF115 regulates phagosome maturation and host response to bacterial infectionThe EMBO journalDecember 1, 2022Bilkei-Gorzo O, Heunis T, Marín-Rubio JL, Cianfanelli FR, Raymond BBA, Inns J, Fabrikova D, Peltier J, Oakley F, Schmid R, et al.were purchased from Cell Signalling technologies: Vimentin (#5741). EEA1 (#2411), Rab7 (#9367), iNOS (#2977), LAMP1 (#9091). Antibodies purchased from Abcam were the following: Cathepsin D (ab75852), Ubiquitin

(13) Dental Pulp-Derived Stem Cells Reduce Inflammation, Accelerate Wound Healing and Mediate M2 Polarization of Myeloid CellsBiomedicinesAugust 17, 2022Anderson S, Prateeksha P, Das H.Cell Signaling, Danvers, MA, USA), ERK1/2 (cat# 4695, Cell Signaling, Danvers, MA, USA), Nos-2 (cat # 2977, Cell Signaling, Danvers, MA, USA), IDO (cat# 86630, Cell Signaling, Danvers, MA, USA), YM-1 (cat# ab93034,

(14) Adipose tissue-derived mesenchymal stem cells' acellular product extracellular vesicles as a potential therapy for Crohn's diseaseJournal of cellular physiologyJuly 1, 2022Altemus J, Dadgar N, Li Y, Lightner AL.(ERK) (9102), Phospho‐MEK1/2 (9154), MEK1/2 (8727), STAT3 (4904), cleaved caspase‐3 (9661), NOS (pan) (2977) were purchased from Cell Signaling Technologies and Direct horseradish peroxidase (HRP) anti‐β‐actin

(15) Gene-selective transcription promotes the inhibition of tissue reparative macrophages by TNFLife science allianceApril 1, 2022Dichtl S, Sanin DE, Koss CK, Willenborg S, Petzold A, Tanzer MC, Dahl A, Kabat AM, Lindenthal L, Zeitler L, et al.(56554), Stat6 (5397), pJNK (9251), JNK (9252), pERK (9101), ERK (9102), p-p38 (9211), p38 (9212), Nos2 (2977), pJunB (8053), JunB (3753), p-cJun (9261), and cJun (9165) antibodies were obtained from Cell Signaling Technology

(16) Mitochondrial Dynamics in SARS-COV2 Spike Protein Treated Human Microglia: Implications for Neuro-COVIDJournal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune PharmacologyDecember 1, 2021Clough E, Inigo J, Chandra D, Chaves L, Reynolds JL, Aalinkeel R, Schwartz SA, Khmaladze A, Mahajan SD.monoclonal (3C144); (Cat # sc-71247, Santa Cruz Biotechnology, Inc.), NOS (pan –NOS antibody, Cat #2977, rabbit polyclonal, Cell signaling technologies), TNF alpha antibody [TNF706] (Cat # GTX108585; mouse

(17) Polylactic Acid and Polycaprolactone Blended Cosmetic Microneedle for Transdermal Hispidin Delivery SystemApplied SciencesMarch 19, 2021Seungyeon Lee, Jihye Lee, Kanghyun Choi, Hyoseon Kim, Youngkum Park, Juhee Yoon, June Hyun Kim, Seongwoo Ryutransferred to a PVDF membrane (BR162-0177, Bio Rad, Seoul, Korea), and immunoblotted with anti-iNOS (#2977S, Cell Signaling, Massachusetts, USA), anti-COX-2 (#12282S, Cell Signaling, USA), or anti-β-Actin (Ab8227,

(18) 9‑Hydroxy‑isoegomaketone inhibits LPS‑induced NO and inflammatory cytokine production in RAW264.7 cellsMolecular medicine reportsMarch 1, 2021Kim HM, Nam B, Paudel SB, Nam JW, Han AR, Jeong HG, Jin CH.were obtained from Santa Cruz Biotechnology, Inc. Rabbit polyclonal antibodies against iNOS (cat. no. 2977S), Nrf2 (cat. no. 12721S) and lamin B (cat. no. 13435S) were purchased from Cell Signaling Technology,

(19) Paroxetine suppresses reactive microglia-mediated but not lipopolysaccharide-induced inflammatory responses in primary astrocytesJournal of NeuroinflammationDecember 1, 2020Xiong Zhang, Lan-Bing Zhu, Jia-Hui He, Hong-Qiu Zhang, Shu-Ya Ji, Chao-Nan Zhang, Na-Na Hou, Chen-Ping Huang, Jian-Hong Zhu(9252), p-p38 (9211), p38 (9212), p-ERK1/2 (9101), ERK1/2 (9102), p-STAT3 (9145), STAT3 (12640), iNOS (2977) and β-actin (4970), anti-rabbit (7074) and anti-mouse (7076) secondary antibodies, and LumiGLO® Reagent

(20) Nerve damage induced skeletal muscle atrophy is associated with increased accumulation of intramuscular glucose and polyol pathway intermediatesScientific ReportsDecember 1, 2020Henning Tim Langer, Shoaib Afzal, Stefan Kempa, Simone Spulerand the sample size increased (n = 6)18. The following antibodies were used: IRS1 (#2382), pan NOS (#2977), GSK3 α/β (#9331), GS (#3893), Annexin A2 (#8235), LC3B (#2775) (Cell Signaling, USA), GLUT1 (#7903),

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NOS (pan) Antibody

NOS (pan) Antibody from Cell Signaling Technology

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Cell Signaling Technology

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NOS (pan) Antibody from Cell Signaling Technology

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