Anti-ARMET/ARP antibody ab67271 from Abcam

Product Specs
ItemAnti-ARMET/ARP antibody
CompanyAbcam
Price Pricing Info Supplier Page View Company Product Page
Catalog Numberab67271
Antibody TypePrimary
Quantity100 UG
HostRabbit
IsotypeIgG
ClonalityPolyclonal
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/TagUnconjugated
Concentration1 mg/ml
Gene NameManf
ReactivityHuman, Mouse, Rat
TargetARMET

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Abcam

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Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) MANF brakes TLR4 signaling by competitively binding S100A8 with S100A9 to regulate macrophage phenotypes in hepatic fibrosisActa Pharmaceutica Sinica BOctober 1, 2023Chao Hou, Dong Wang, Mingxia Zhao, Petek Ballar, Xinru Zhang, Qiong Mei, Wei Wang, Xiang Li, Qiang Sheng, Jun Liu, et al.study23. Briefly, immunoprecipitation was performed on HepG2 cell lysates using anti-MANF antibody (ab67271, Abcam) or isotype control antibody (CST3900, Cell Signaling Technology). Protein samples were used

(2) Cold Plasma Irradiation Attenuates Atopic Dermatitis via Enhancing HIF-1α-Induced MANF Transcription ExpressionFrontiers in ImmunologyJuly 14, 2022Tao Sun et al.anti-Bip (Abcam, ab21685); anti-CHOP (Abcam, ab11419); anti-HO-1 (Abcam, ab52947); anti-MANF (Abcam, ab67271); anti-GAPDH (Abcam, ab3285); anti-HIF-1α (Abcam, ab243860); Anti-CD163 (Abcam, ab182422); Goat Anti-Rabbit IgG H&L (HRP

(3) Mesencephalic astrocyte-derived neurotrophic factor reprograms macrophages to ameliorate acetaminophen-induced acute liver injury via p38 MAPK pathwayCell Death & DiseaseFebruary 2, 2022Xin Hou et al.(H&E) or subjected to immunohistochemical staining using anti-MANF antibody (Abcam, Cambridge, MA, USA, ab67271), anti-Cyp2E1 antibody (Abcam, Cambridge, MA, USA, ab28146) according to the manufacturers’ protocols.

(4) New Proteins Contributing to Immune Cell Infiltration and Pannus Formation of Synovial Membrane from Arthritis DiseasesInternational journal of molecular sciencesDecember 31, 2021de Seny D, Baiwir D, Bianchi E, Cobraiville G, Deroyer C, Poulet C, Malaise O, Paulissen G, Kaiser MJ, Hauzeur JP, et al.antibody against LAP3 (Abcam, #ab154809, dil 1/100), DNAJB11 (Abcam, #ab224082, dil 1/200), MANF (Abcam, #ab67271, dil 1/400), SCARA5 (Abcam, #ab118894, dil 1/200), eIF3k (Thermofisher, #PA5-27593, dil 1/1000), LCP1

(5) Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagyNature communicationsJune 24, 2021Vanhoutte D, Schips TG, Vo A, Grimes KM, Baldwin TA, Brody MJ, Accornero F, Sargent MA, Molkentin JD.were quantified using the Li-Cor Image Studio software. Primary antibodies used were: Armet (Abcam, #ab67271 at 1:1000), p-AKT ser473 (Cell Signaling Technology, #9271 at 1:1000), AKT (Cell Signaling Technology,

(6) MANF Produced by MRL Mouse-Derived Mesenchymal Stem Cells Is Pro-regenerative and Protects From OsteoarthritisFrontiers in Cell and Developmental BiologyMarch 2, 2021Gautier Tejedor, Patricia Luz-Crawford, Audrey Barthelaix, Karine Toupet, Sébastien Roudières, François Autelitano, Christian Jorgensen, Farida Djouadprotein expression profile of each sample. Blots were probed with antibodies directed against MANF (Abcam, Catalog # ab67271), growth arrest specific 6 (GAS6, R&D Systems, Catalog # MAB986), neudesin neurotrophic factor (NENF;

(7) Increased MANF Expression in the Inferior Temporal Gyrus in Patients With Alzheimer DiseaseFrontiers in aging neuroscienceJanuary 1, 2021Liu XC, Qi XH, Fang H, Zhou KQ, Wang QS, Chen GH.MANF Antibodies To ensure that the rabbit polyclonal anti-MANF antibody used in this study (ARMET/ARP; cat. no. Ab67271; Abcam, Cambridge, United Kingdom) could detect MANF in human brain specimens, we tested its specificity

(8) Neuroprotective effects of verbascoside against Alzheimer’s disease via the relief of endoplasmic reticulum stress in Aβ-exposed U251 cells and APP/PS1 miceJournal of NeuroinflammationDecember 1, 2020Chunyue Wang, Xueying Cai, Ruochen Wang, Siyu Zhai, Yongfeng Zhang, Wenji Hu, Yizhi Zhang, Di WangUSA" 3,tau (phosphor S396),,ab109390,1:4000,Immunohistochemical,Abcam,"Cambridge, MA, USA" 4,MANF,,ab67271,1:400,Immunohistochemical,Abcam,"Cambridge, MA, USA" 5,MANF,20 kDa,ab67271,1:1000,Western blot,Abcam,"Cambridge,

(9) Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) Regulates Neurite Outgrowth Through the Activation of Akt/mTOR and Erk/mTOR Signaling PathwaysFrontiers in Molecular NeuroscienceSeptember 24, 2020Wen Wen, Yongchao Wang, Hui Li, Hong Xu, Mei Xu, Jacqueline A. Frank, Murong Ma, Jia LuoStates). The following antibodies were used: anti-α-tubulin (T5168, Sigma-Aldrich); anti-ARMET/ARP (MANF) (ab67271 for C-terminus and ab67203 for N-terminus, Abcam, Cambridge, MA, United States); anti-HA-Tag (CST 3724),

(10) Mesencephalic Astrocyte-Derived Neurotrophic Factor, a Prognostic Factor of Cholangiocarcinoma, Affects Sorafenib Sensitivity of Cholangiocarcinoma Cells by Deteriorating ER StressOncoTargets and TherapySeptember 16, 2020Jingyi He, Guangbing Li, Xihan Liu, Liye Ma, Jiayao Zhang, Shunzhen Zheng, Jianping Wang, Jun Liucitrate buffer for antigen retrieval, the sections were incubated with primary antibody against ARMET (ab67271, Abcam, CA, USA; dilution 1:100, ARMET is the alternative name for MANF) at 4°C overnight and then secondary

(11) Mechanisms of Cong Rong Shu Jing Compound Effects on Endoplasmic Reticulum Stress in a Rat Model of Parkinson's DiseaseEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2020Lin Y, Tang L, Huang P, Liu T, Zhong J, Xu Q, Li X, Lin Y, Xiao S, Cai J.Wuhan Sanying Company); and mesencephalic astrocyte-derived neurotrophic factor (MANF) antibody (no. Ab67271), tyrosine hydroxylase (TH) antibody (no. Ab112), GRP78 antibody (no. Ab108613), ASK1 antibody (no.

(12) Gamma Entrainment Binds Higher-Order Brain Regions and Offers NeuroprotectionNeuronJune 5, 2019Adaikkan C, Middleton SJ, Marco A, Pao PC, Mathys H, Kim DN, Gao F, Young JZ, Suk HJ, Boyden ES, et al.,Santa Cruz Biotechnology,"Cat# sc-32233, 6C5" c-Fos (1:500),Santa Cruz Biotechnology,Cat# sc-52 ARMET (1:1000),Abcam,Cat# ab67271 Total Tau (1: 1000),Santa Cruz Biotechnology,"Cat# sc-5587, H-150" p35/p25 (1:1000),In house made,N/A EGFP

(13) Mesencephalic astrocyte-derived neurotrophic factor (MANF) protects against Aβ toxicity via attenuating Aβ-induced endoplasmic reticulum stressJournal of neuroinflammationFebruary 13, 2019Xu S, Di Z, He Y, Wang R, Ma Y, Sun R, Li J, Wang T, Shen Y, Fang S, et al.at 4 °C overnight with the following primary antibodies: rabbit anti-MANF antibody (1: 1000, Abcam, ab67271), rabbit anti-BiP antibody (1:1000, proteintech, 11587-1-ap), rabbit anti-CHOP antibody (1:1000, proteintech,

(14) Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instabilityNature communicationsJanuary 8, 2019Schips TG, Vanhoutte D, Vo A, Correll RN, Brody MJ, Khalil H, Karch J, Tjondrokoesoemo A, Sargent MA, Maillet M, et al.glyceraldehyde 3-phosphate dehydrogenase (Gapdh (Fitzgerald, #10R-G109A at 1:10000)), Armet (Abcam, #ab67271 at 1:1000), ATF6α (Abcam, #ab37149 at 1:1000), calreticulin (Cell Signaling Technology, #2891; at 1:1000),

(15) Conserved roles of C. elegans and human MANFs in sulfatide binding and cytoprotectionNature CommunicationsDecember 1, 2018Meirong Bai et al.include anti-V5 (ab3792, dilution 1:1000; Abcam), anti-His (6AT18, 1:1000; Sigma), anti-MANF C-terminus (ab67271, dilution 1:1000; Abcam). The antibody targeting the N-terminal Saposin-like domain of MANF (ab129602,

(16) Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) Protects Against Neuronal Apoptosis via Activation of Akt/MDM2/p53 Signaling Pathway in a Rat Model of Intracerebral HemorrhageFrontiers in Molecular NeuroscienceMay 29, 2018Weilin Xu, Liansheng Gao, Tao Li, Jingwei Zheng, Anwen Shao, Jianmin Zhangtemperature and incubated at 4◦ C overnight with primary antibodies: rabbit anti-MANF (1:500, Abcam ab67271), anti-Caspase3 (1:200, Abcam ab13847), mouse anti-NeuN (1:500, Abcam ab177487). After that, secondary

(17) MANF regulates hypothalamic control of food intake and body weightNature communicationsSeptember 18, 2017Yang S, Yang H, Chang R, Yin P, Yang Y, Yang W, Huang S, Gaertig MA, Li S, Li XJ.DK076169. Antibodies and plasmids Primary antibodies used in this study include: MANF (LSBio, C53208, B2688; Abcam, ab67271; 1:1000), Actin (Sigma, A5060; 1:10,000), HA (Cell signaling, 2367, 3724; Roche, 12CA5; 1:10,000), Vinculin

(18) Thrombospondin expression in myofibers stabilizes muscle membraneseLifeSeptember 26, 2016Vanhoutte D, Schips TG, Kwong JQ, Davis J, Tjondrokoesoemo A, Brody MJ, Sargent MA, Kanisicak O, Yi H, Gao QQ, et al.primary antibodies used in this study included: α-actinin (sarcomeric, A7811; 1/1000); Armet (Abcam, ab67271; 1:1000); BiP (Cell Signaling Technology, Danvers, MA, 3177; 1:1000); calreticulin (Cell Signaling Technology,

(19) Assessing the contribution of thrombospondin-4 induction and ATF6α activation to endoplasmic reticulum expansion and phenotypic modulation in bladder outlet obstructionScientific reportsSeptember 1, 2016Krawczyk KK, Ekman M, Rippe C, Grossi M, Nilsson BO, Albinsson S, Uvelius B, Swärd K.

(20) Identification of MANF as a protein interacting with RTN1-CActa biochimica et biophysica SinicaFebruary 1, 2015Chen L, Wan L, Du J, Shen Y.

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Anti-ARMET/ARP antibody from Abcam

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