Fig 1: Human podocytes express extracellular L-BAIBA stimulation-sensitive MRGPRD. (A) MRGPRD protein expression in podocytes before and after L-BAIBA treatment (10 µM) for 24 h, 2 days, and 5 days. n = 9. *p < 0.05, **p < 0.01, ***p < 0.001. (B) Representative immunoblot membranes. (C) MRGPRD mRNA expression levels in podocytes before and after L-BAIBA treatment (10 µM) for 24 h, 2 days, and 5 days. n = 7–9. *p < 0.05, **p < 0.01. (D) Representative agarose gel with PCR products. (E) Confocal images of podocytes immunostained for MRGPRD and the podocyte marker synaptopodin. Scale bar = 10 µm.