Fig 2: PAMP stimulation induces expression of negative regulators of TLR signaling in human tracheobronchial epithelial cells (TBEC). mRNA expression of negative regulators Tollip (a), A20 (b) and IRAK-M (c) in TBEC in the absence (-) and presence of LPS or Poly(I:C) (PIC) at 4, 24 and 48 h. N = 8 donor subjects. Median values are shown as horizontal lines. Densitometric analysis of Tollip (d) and A20 (e) western blots on lysates of TBEC in the absence (-) or presence of treatments with LPS, PIC at 4, 24 and 48 h. ß-actin was included as a protein loading control to normalize Tollip or A20 expression. N = 3 donor subjects (additional blots in Additional file 1: Figure S2)

Fig 3: PAMP stimulation induces expression of negative regulators of TLR signaling in alveolar macrophages. mRNA expression of the negative regulators Tollip (a), A20 (b) and IRAK-M (c) in macrophages in the absence (-) and presence of LPS, Poly(I:C) (PIC) at 4, 24 and 48 h. N = 8 donor subjects. Median values are shown as horizontal lines. Densitometric analysis of Tollip (d) and A20 (e) western blots on lysates of macrophages in the absence (-) or presence of treatments with LPS, PIC at 4, 24 and 48 h. ß-actin was included as a protein loading control to normalize Tollip or A20 expression. N = 3 donor subjects (additional blots in Additional file 1: Figure S2)