ASCL2 Rabbit Polyclonal Antibody orb155740 from Biorbyt

Product Specs
ItemASCL2 Rabbit Polyclonal Antibody
CompanyBiorbyt
Price $250.00Supplier Page
Catalog Numberorb155740
Quantity50 ul, 100 ul, 200 ul
ApplicationsFC, ICC, IF, IHC-Fr, IHC-P
Conjugate/TagUnconjugated
ReactivityHuman, Mouse
TargetASCL2
ClonalityPolyclonal
FormatLiquid
ImmunogenKLH conjugated synthetic peptide derived from Human ASCL2 (51-120/193aa)
Molecular Weight22 kDa
IsotypeIgG
HostRabbit
Concentration1mg/ml
NCBI Full Gene Nameachaete-scute family bHLH transcription factor 2
NCBI Gene AliasesASH2, HASH2, MASH2, bHLHa45
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

Specifications/Features

Application Dilutions: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test

Questions and Answers

Can this antibody be used to study ASCL2 in hepatoblastoma research?

Yes, the ASCL2 antibody has been utilized in research related to hepatoblastoma, as indicated by its role in the regulation of gene expression and its association with epigenetic dysregulations in pediatric liver tumors. Specifically, studies have shown that ASCL2 is a regulatory transcription factor involved in the Wnt signaling pathway, which is relevant to the molecular profiling of hepatoblastoma and understanding its carcinogenesis ().

What applications is this antibody suitable for in cell biology experiments?

The ASCL2 antibody is suitable for various applications including ELISA, flow cytometry, immunocytochemistry, immunofluorescence, and both frozen and paraffin-embedded tissue immunohistochemistry. This versatility allows researchers to utilize the antibody in multiple experimental contexts to study ASCL2 expression and function.

What species does this antibody react with, and how might that influence my experimental design?

This antibody is reactive with both human and mouse species. This cross-reactivity allows for comparative studies between human and mouse models, which can be particularly useful in translational research where insights from mouse models are applied to human biology.

What concentration should I use for immunocytochemistry with this antibody?

For immunocytochemistry, the recommended dilution for the ASCL2 antibody is 1:100. This concentration has been shown to be effective in previous studies, ensuring optimal detection of ASCL2 in cellular contexts.

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(1) Rectal Epithelial Stem Cell Kinetics in Acute Radiation ProctitisInternational journal of molecular sciencesOctober 19, 2024Ghosh S, Morita A, Nishiyama Y, Sakaue M, Fujiwara K, Morita D, Sonoyama Y, Higashi Y, Sasatani M.MN, USA, 1:400), Olfm4 (clone 39141, Cell Signaling Technology, Danvers, MA, USA, 1:1000) and Ascl2 (orb155740, Biorbyt, Cambridge, UK, 1:1000) diluted in 1% BSA in PBS-T, were added to the section and incubated

(2) Retinoic Acid-Induced 2 (RAI2) Is a Novel Antagonist of Wnt/β-Catenin Signaling Pathway and Potential Biomarker of Chemosensitivity in Colorectal CancerFrontiers in oncologyJanuary 1, 2022Zhang W, Kong L, Zhu H, Sun D, Han Q, Yan B, Cui Z, Zhang W, Zhang S, Kang X, et al.

(3) ASCL2 Affects the Efficacy of Immunotherapy in Colon Adenocarcinoma Based on Single-Cell RNA Sequencing AnalysisFrontiers in immunologyJanuary 1, 2022Wu L, Sun S, Qu F, Liu X, Sun M, Pan Y, Zheng Y, Su G.

(4) Genetic and epigenetic basis of hepatoblastoma diversityNature communicationsSeptember 20, 2021Nagae G, Yamamoto S, Fujita M, Fujita T, Nonaka A, Umeda T, Fukuda S, Tatsuno K, Maejima K, Hayashi A, et al.was raised against a 61–110 amino acid peptide sequence that was mapped in the middle of ASCL2 (No. ORB155740, Biorbyt Ltd, Cambridge, UK) and used at a concentration of 2.5 μg/ml. Tissue preparation The tissues

(5) Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic nicheCell reportsSeptember 7, 2021He X, Smith SE, Chen S, Li H, Wu D, Meneses-Giles PI, Wang Y, Hembree M, Yi K, Zhao X, et al.A2,Abcam,ab178677;RRID:AB_1140698 Annexin A1,Abcam,ab214486;RRID:AB_722804 Ascl2 antibody,Biorbyt,orb155740;RRID:AB_10865613 E-Cadherin (24E10) Rabbit mAb,Cell Signaling Technology,3195;RRID:AB_2291471 PD-1

(6) Protective C allele of the single-nucleotide polymorphism rs1335532 is associated with strong binding of Ascl2 transcription factor and elevated CD58 expression in B-cellsBiochimica et biophysica acta. Molecular basis of diseaseOctober 1, 2018Mitkin NA, Muratova AM, Korneev KV, Pavshintsev VV, Rumyantsev KA, Vagida MS, Uvarova AN, Afanasyeva MA, Schwartz AM, Kuprash DV.

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(10) Paradoxical caloric response in altered states of consciousness. Clinical and EEG correlations in toxic metabolic encephalopathiesNeurologyAugust 1, 1972Maccario M, Backman JR, Korein J.

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