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Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control
Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control from Abcam
Be the first to write a review!
Citations:
(44)
Supplier Page
Supplier Page from
Abcam for
Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab176560
Quantity
10 UL, 40 UL, 100 UL
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Conjugate/Tag
Unconjugated
Isotype
IgG
Clone
EPR13478(B)
Host
Rabbit
Antibody Type
Primary
NCBI Gene Aliases
ALS22, H2-ALPHA, TUBA1
Clonality
Monoclonal
Gene Name
TUBA4A
Reactivity
Human
Target
alpha Tubulin
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (44)
(1)
In silico and in vitro analyses of a novel FoxO1 agonist reducing Aβ levels via downregulation of BACE1
CNS neuroscience & therapeutics
March 1, 2024
Lv MT, Wang HC, Meng XW, Shi YT, Zhang YM, Shan LL, Shi RL, Ni TJ, Duan YC, Yang ZJ,
et al
.
A8717
;
Sigma–Aldrich
), anti‐ADAM10 (
1:1000
, #
14194
S;
Cell Signaling Technologies
), anti‐PS1 (
1:1000
, #
ab176560
;
Abcam
), anti‐p‐
FoxO1
(
1:1000
, #
9461
S;
Cell Signaling Technology
), or anti‐GAPDH (
1:5000
, # AB0037;
(2)
Integrated machine learning identifies epithelial cell marker genes for improving outcomes and immunotherapy in prostate cancer
Journal of Translational Medicine
November 4, 2023
Weian Zhu, Hengda Zeng, Jiongduan Huang, Jianjie Wu, Yu Wang, Ziqiao Wang, Hua Wang, Yun Luo, Wenjie Lai
USA). The primary antibodies used were anti-TMED3 (
Abcam
,
ab223175
, USA) and anti-α-Tubulin (
Abcam
,
ab176560
, USA). Cell counting kit-8 (CCK-8)
assay
The CCK-8
assay
(Dojindo, Japan) was conducted following
(3)
Hydrogel platform facilitating astrocytic differentiation through cell mechanosensing and YAP-mediated transcription
Materials Today Bio
October 1, 2023
Zhongqian Liu, Shijie Mao, Yubin Hu, Feng Liu, Xiaowei Shao
western blot
were anti-
GFAP
(130300, Invitrogen), anti-S100β (
ab52642
, Abcam), and anti-α-Tubulin (
ab176560
,
Abcam
). Secondary antibodies were HRP-conjugated anti-rat IgG (
31470
,
Invitrogen
) and anti-rabbit IgG
(4)
Inhibition of abnormal C/EBPβ/α‐Syn signaling pathway through activation of Nrf2 ameliorates Parkinson's disease‐like pathology
Aging Cell
October 1, 2023
Zefang Lin, Lixuan Huang, Qianqian Cao, Hanyue Luo, Wei Yao, Ji‐chun Zhang
GTX10372
) was purchased from GTX (
GeneTex
); anti‐β‐actin antibody (
ab198991
) and anti‐β‐tubulin antibody (
ab176560
) were purchased from
Abcam
; anti‐
GAPDH
antibody (
AF7021
) was purchased from
Affinity
. The HRP‐conjugated
(5)
Stem Cell Properties of Gastric Cancer Stem-Like Cells under Stress Conditions Are Regulated via the c-Fos/UCH-L3/β-Catenin Axis
Molecules and Cells
August 31, 2023
Jae Hyeong Lee, Sang-Ah Park, Il-Geun Park, Bo Kyung Yoon, Jung-Shin Lee, Ji Min Lee
,
Santa Cruz Biotechnology
,#
sc-58595
Anti-Lamin A/C antibody,
Cell Signaling Technology
,#
2032S
Anti-α-tubulin antibody,
Abcam
,#
ab176560
Anti-c-Fos antibody,
Cell Signaling Technology
,#
2250S
Anti-FLAG M2 antibody,Sigma,#3165 Anti-HA antibody,BioLegend,#MMS-101R Anti-GFP(B20)
(6)
Regulation of cellular contractile force, shape and migration of fibroblasts by oncogenes and Histone deacetylase 6
Frontiers in Molecular Biosciences
July 20, 2023
Ana López-Guajardo, Azeer Zafar, Khairat Al Hennawi, Valentina Rossi, Abdulaziz Alrwaili, Jessica D. Medcalf, Mark Dunning, Niklas Nordgren, Torbjörn Pettersson, Ian D. Estabrook,
et al.
as follows: anti-
mouse
-acetylated-α-tubulin (
T6793
,
Sigma Aldrich
) (
1:2,000
), anti-rabbit-α-tubulin (
ab176560
,
Abcam
) (
1:2,000
), anti-mouse-GAPDH (60004, Proteintech) (
1:2,000
), and anti-rabbit-HDAC6 (NB100-56343,
(7)
Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma
Cell death & disease
July 17, 2023
Guo W, Wang X, Lu B, Yu J, Xu M, Huang R, Cheng M, Yang M, Zhao W, Zou C.
cleaved Capase-3 (
9661S
,
Cell Signaling Technology
),
SLC7A11
(
12691S
,
Cell Signaling Technology
), Tubulin (
ab176560
,
Abcam
) and
4-Hydroxynonenal
(
ab46545
,
Abcam
),
Caspase-3
(
ab184787
,
Abcam
), and corresponding
horseradish
(8)
The histone H3/H4 chaperone CHAF1B prevents the mislocalization of CENP-A for chromosomal stability
Journal of Cell Science
May 15, 2023
Roshan L. Shrestha, Vinutha Balachandra, Jee Hun Kim, Austin Rossi, Pranathi Vadlamani, Subhash Chandra Sethi, Laurent Ozbun, Shinjen Lin, Ken Chin-Chien Cheng, Raj Chari,
et al.
anti-
CHAF1A
(
ab126625
,
Abcam
) and
anti-RbAp48
(
ab1765
,
Abcam
) at
1:500
dilution;
mouse
anti-α-tubulin (
ab176560
,
Abcam
), rabbit anti-DAXX (25C12, Cell Signaling Technology), rabbit anti-cyclin E1 (
20808
,
Cell Signaling Technology
(9)
Three-dimensional growth reveals fine-tuning of 5-lipoxygenase by proliferative pathways in cancer
Life science alliance
May 1, 2023
Göbel T, Goebel B, Hyprath M, Lamminger I, Weisser H, Angioni C, Mathes M, Thomas D, Kahnt AS.
,
Santa Cruz Biotechnology
Rictor
,
sc-271081
,
Santa Cruz Biotechnology
α-Tubulin,
sc-5286
,
Santa Cruz Biotechnology
α-Tubulin,
ab176560
,
Abcam
Antibodies used for
immunoblot
analysis.
(10)
Mechanism for exercise-mediated prevention against muscle wasting on extensor digitorum longus muscle in Spontaneously Diabetic Torii fatty rats
The Journal of Physiological Sciences
April 4, 2023
Hitoshi Kotake, Yuji Ogura, Shohei Yamada, Kazuho Inoue, Shiika Watanabe, Daisuke Ichikawa, Takeshi Sugaya, Keiichi Ohata, Yasunori Natsuki, Seiko Hoshino,
et al.
Thermo Fisher Scientific
, Rockford, IL, USA). We also used a rabbit monoclonal antibody to α-tubulin (
ab176560
;
1:4000
-8000,
Abcam
) to detect α-tubulin on the same
membranes
. After
incubation
with
horseradish
peroxidase-conjugated
(11)
Autophagy controls mucus secretion from intestinal goblet cells by alleviating ER stress
Cell Host & Microbe
March 8, 2023
Maria Naama, Shahar Telpaz, Aya Awad, Shira Ben-Simon, Sarina Harshuk-Shabso, Sonia Modilevsky, Elad Rubin, Jasmin Sawaed, Lilach Zelik, Mor Zigdon,
et al.
overnight with the following primary antibodies: α-GADD153/CHOP (SCBT sc-166682) and α-Tubulin (
Abcam
ab176560
). After washing,
membranes
were incubated with the species-appropriate HRP-conjugated secondary antibodies.
(12)
Poison cassette exon splicing of SRSF6 regulates nuclear speckle dispersal and the response to hypoxia
Nucleic acids research
January 25, 2023
de Oliveira Freitas Machado C, Schafranek M, Brüggemann M, Hernández Cañás MC, Keller M, Di Liddo A, Brezski A, Blümel N, Arnold B, Bremm A,
et al
.
(
NBP2-04144
,
Novus Biologicals
), α-
mouse
BNIP3
(
ab10433
,
Abcam
) and α-rabbit alpha-tubulin antibody (
ab176560
;
Abcam
). Image acquisition and quantification were performed using the ImageLab software (Bio-Rad). SRSF6–GFP
(13)
Angiotensin II type 1a receptor deficiency alleviates muscle atrophy after denervation
Scientific Reports
January 10, 2023
Suguru Takayama, Kazuho Inoue, Yuji Ogura, Seiko Hoshino, Takeshi Sugaya, Keiichi Ohata, Hitoshi Kotake, Daisuke Ichikawa, Minoru Watanabe, Kenjiro Kimura,
et al.
#
3033
;
1:1000
;
Cell Signaling Technology
). We also used a rabbit monoclonal antibody to α-tubulin (
ab176560
;
1:4000
,
Abcam
) to detect α-tubulin on the same
membranes
. After
incubation
with
horseradish
peroxidase-conjugated
(14)
AZD6738 Inhibits fibrotic response of conjunctival fibroblasts by regulating checkpoint kinase 1/P53 and PI3K/AKT pathways
Frontiers in Pharmacology
September 20, 2022
Longxiang Huang, Qin Ye, Chunlin Lan, Xiaohui Wang, Yihua Zhu
(
AF5718
,
goat
polyclonal antibody, 1 mg/ml;
R&D Systems
, Minneapolis, MN, United States), and α-Tubulin (
ab176560
, rabbit monoclonal antibody,
1:1,000
;
Abcam
, Cambridge, MA, United States) overnight at 4 °C. After
(15)
Activating transcription factor 4 aggravates angiotensin II-induced cell dysfunction in human vascular aortic smooth muscle cells via transcriptionally activating fibroblast growth factor 21
The Korean Journal of Physiology & Pharmacology
September 1, 2022
Ke Tao, Ming Li, Xuefeng Gu, Ming Wang, Tianwei Qian, Lijun Hu, Jiang Li
1:1,000
),
ATF4
(
ab184909
,
1:1,000
), α-SMA (
ab124964
,
1:10,000
), SM22α (
ab155272
,
1:1,000
), Tubulin (
ab176560
,
1:2,000
) (all from
Abcam
, Cambridge, MA, USA), followed by
incubation
with the secondary antibody (
ab96899
;
(16)
Butein-instigated miR-186-5p-dependent modulation of TWIST1 affects resistance to cisplatin and bioenergetics of Malignant Pleural Mesothelioma cells
Cancer Drug Resistance
July 3, 2022
Mario Cioce, Daniela Rutigliano, Annamaria Puglielli, Vito Michele Fazio
membranes
were blocked and then probed with antibodies against TWIST1 (
ab50887
) and anti-alpha tubulin (
ab176560
) as a loading control (
Abcam
, Cambridge, MA, USA). After washing, the blots were incubated with
horseradish
(17)
HuR Affects the Radiosensitivity of Esophageal Cancer by Regulating the EMT-Related Protein Snail
Frontiers in Oncology
May 19, 2022
Yan Hu, Qing Li, Ke Yi, Chi Yang, Qingjun Lei, Guanghui Wang, Qianyun Wang, Xiaohui Xu
Inc., Danvers, MA, USA),β-actin (
1:2000
, GB1201, Servicebio, Woburn, MA, USA) and Tubulin (
1:2000
,
ab176560
,
Abcam
, Cambridge, UK). Goat anti-
mouse
primary antibodies (
1:1000
, A0208) or
goat
anti-rabbit secondary
(18)
Inhibitors of class I HDACs and of FLT3 combine synergistically against leukemia cells with mutant FLT3
Archives of toxicology
January 1, 2022
Wachholz V, Mustafa AM, Zeyn Y, Henninger SJ, Beyer M, Dzulko M, Piée-Staffa A, Brachetti C, Haehnel PS, Sellmer A,
et al
.
Biosciences p-
STAT5
(Tyr694),
ma5-14973
,
Thermo Fisher
thymidylate synthase,#
9045
,
Cell Signaling
α-tubulin,
Ab176560
,
Abcam
UBCH8
,
AP2118a
,
Abgent
Vinculin,
sc-7336
,
Santa Cruz
WEE1
,
sc-5285
,
Santa Cruz
List of antibodies
(19)
Rational Development and Characterization of a Ubiquitin Variant with Selectivity for Ubiquitin C-Terminal Hydrolase L3
Biomolecules
January 1, 2022
Hewitt CS, Das C, Flaherty DP.
Cambridge, UK);
UCHL3
-D25E6 (Cell Signaling Technologies),
Ab126621
(
Abcam
); Alpha Tubulin-
Ab7291
(
Abcam
) or
Ab176560
(
Abcam
). Fluorescent secondary antibodies (Licor IRDye 680RD Goat anti-Rabbit and Licor IRDye 800CW
(20)
Angiotensin II type 1a receptor loss ameliorates chronic tubulointerstitial damage after renal ischemia reperfusion
Scientific Reports
December 1, 2021
Yoko Fujita et al.
detect the expression of α-tubulin on the same
membranes
, a rabbit monoclonal antibody to α-tubulin (
ab176560
;
1:4000
,
Abcam
) was used. A
horseradish
peroxidase-conjugated anti-rabbit antibody (
ab97051
,
Abcam
)
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Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control from Abcam
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