Fig 2: Analysis of fibronectin associated gene expression and protein kinase phosphorylation changes in HPV8-E7 positive keratinocytes. (A) PrimePCR results for N/TERT-8E7 grown on fibronectin for 96 h (n = 4). (B) Representative immunoblots of RIPA extracts from N/TERT-8E7 tested for total Fyn and pTyr530-Fyn (n = 3). HuT78 whole cell lysates served as a positive control. Equal loading was confirmed by immunoblotting for GAPDH. (C) Protein kinase phosphorylation patterns in N/TERT-8E7 cells cultured on fibronectin for 96 h were analyzed using the Proteome Profiler Human Phospho- Kinase Array Kit (RnD Systems). The cut-off value for up- and down-regulated genes was set to fivefold. The dashed line indicates the 15-fold change. (D) Representative immunoblots of RIPA extracts of N/TERT-8E7 tested for total Lyn and pTyr397-Lyn (n = 3). HuT78 whole cell lysates served as a positive control. Equal loading was confirmed by immunoblotting for GAPDH.

Fig 3: Effect of LUX and IB on LYN and CD79A in SU-DHL-6 cells.SU-DHL-6 cells were pre-treated for 2 h with either LUX or IB and then stimulated with anti-human IgM or left unstimulated. (A) Western blot analysis of whole cell lysates for p-CD79A (Y182), CD79A, p-LYN (Y397)/LCK (Y394)/HCK (Y411)/BLK (Y389), LYN and GAPDH; representative blots are shown. (B-D) Quantification of intensity of bands in Western blots shown in A. Data is mean ± SEM of data from 2 independent experiments.