Fig 1: ALTA® extended-release vaccine containing the germline-targeting HIV-1 immunogen N332-GT5 gp140 outperforms a single administration of the liquid vaccine formulationA. Total anti-N332-GT5 gp140 IgG titers after a single administration of 50-cycle ALTA® on D0 at indicated doses of N332-GT5 gp140. Plots shows geometric mean titer (n=8/group) +/− 95% confidence interval.B. Analysis of fluorescent signal at site of injection following administration of 50-cycle ALTA® containing fluorescently-labeled N332-GT5 gp140. Plot shows mean +/− SEM of percent of fluorescent radiant efficiency (p/s)/(μW/cm2) relative to radiant efficiency at 24 hr timepoint measured at site of injection for 50-cycle ALTA® N332-GT5 gp140 administered at specified doses (square traces). Total anti-N332-GT5 gp140 IgG titers plotted as geometric mean +/− 95% confidence interval (n=5 mice/group) (triangle traces).C. Kinetics of total anti-N332-GT5 gp140 IgG titers elicited by a single administration of 10 μg N332-GT5, delivered in a liquid formulation (blue) or in 50-cycle ALTA® products with/without the presence of adjuvants in the injection diluent. Plot shows geometric mean titer +/− 95% confidence interval (n=16 mice/group red trace, n=8 mice/group all others)D. Total anti-N332-GT5 gp140 IgG1, IgG2b, IgG2c and IgG3 titers elicited by a single administration of 10 μg N332-GT5 gp140 at week 8 post injection, delivered in a liquid formulation (blue) or in 50-cycle ALTA® products with/without the presence of adjuvants in the injection diluent. Plot shows geometric mean titer +/− 95% confidence interval (n=16 mice/group red trace, n=8 mice/group all others). ** = p < 0.01, * = p < 0.05, data analyzed using Kruskal-Wallis test.E-H. Total anti-N332-GT5 gp140 (E) IgG1, (F) IgG2b, (G) IgG2c and (H) IgG3 titers elicited by a single administration of 10 μg N332-GT5 gp140 at week 8 post injection, delivered in a liquid formulation (blue) or in 50-cycle ALTA® products with/without the presence of adjuvants in the injection diluent. Plot shows geometric mean titer +/− 95% confidence interval (n=16 mice/group red trace, n=8 mice/group all others. **** = p < 0.0001, ** = p < 0.01, * = p < 0.05, ns = p > 0.05, data analyzed using Kruskal-Wallis test.