WB using Anti-SmarcAL1 antibody shows detection of a band ~106 kDa band (arrowhead) corresponding to SmarcAL1 in human derived cultured cell lysates (HeLa nuclear extract lane 1, HeLa lane 2, A431 lane 3, Jurkat lane 4, and 293 lane 5). Approximately 5ug of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SmarcAL1 antibody. SmarcAL1 is the band seen between the 97 and 116kD markers.