Retinae were collected from adult White Leghorn chicken were incubated at RT with 1:250 (1%BSA/.1% saponin PBS) ClC4 antibody (Cat.# TA324834) for 1 hour. Sections were then washed in PBS (3X10minutes) and then treated with secondary antibody (1:500 Cy3) for one hour. After another PBS wash series, sections were coverslipped and antibody labeling was visualized at 20X with a Leica upright microscope using a TRITC filter set and Xenon lamp illumination. (Crousillac et al, 2003)

Immunofluorescence image of cultured chick retinal amacrine (neuronal) cells labeled with CLC4 Antibody (C-term) (TA324834). Data courtesy of Emily McMains, Louisiana State University.