Western blot analysis of TRAF7 in Huvec, MCF7, U2-OS, A431, SHSY-5Y and HeLa cells. Samples were incubated in TRAF7 polyclonal antibody (Product # PA1-41285) using a dilution of 2 µg/mL followed by an anti-rabbit HRP secondary antibody. Separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. Detection: chemiluminescence.