CH25H Polyclonal Antibody bs-6480R from Bioss Inc.

Product Specs
ItemCH25H Polyclonal Antibody
CompanyBioss Inc.
Price $375.00Supplier Page View Company Product Page
Catalog Numberbs-6480R
Quantity100ul, 20uL
ApplicationsIHC, WB, IHC-P, IHC-F, IF, IHC-P
Concentration1ug/ul
Conjugate/TagUnconjugated
ReactivityMouse, Human
TargetCH25H
IsotypeIgG
HostRabbit
Antibody TypePrimary Antibody
ClonalityPolyclonal
Format0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol
NCBI Full Gene Namecholesterol 25-hydroxylase
NCBI Gene AliasesC25H
ImmunogenKLH conjugated peptide, human CH25H

Questions and Answers

Can this antibody be used to study the role of cholesterol 25-hydroxylase in immune responses?

Yes, the CH25H Polyclonal Antibody has been utilized in studies examining its role in modulating immunoglobulin A secretion by plasma cells in the gut. For instance, research has shown that 7α,25-dihydroxycholesterol, produced by epithelial cells, is critical for regulating IgA secretion against commensal and pathogen-derived antigens, highlighting the antibody's relevance in immune response studies ().

What experimental techniques have been successfully employed with this antibody?

The CH25H Polyclonal Antibody has been effectively used in immunoblotting techniques. For example, in a study, protein extracts from intestinal epithelial cells were analyzed using this antibody to quantify cholesterol 25-hydroxylase levels, demonstrating its utility in protein detection and quantification ().

Is there any evidence of this antibody being used in animal models?

Yes, the CH25H Polyclonal Antibody has been used in mouse models to investigate the effects of dietary changes on cholesterol metabolism and immune responses. In one study, it was employed to assess the expression of cholesterol 25-hydroxylase in the jejunum of mice fed a Western diet, indicating its application in in vivo research ().

What species does this antibody react with, and is it suitable for human studies?

The CH25H Polyclonal Antibody is specifically reactive with human proteins, making it suitable for studies involving human samples. Its application in research focused on human cholesterol metabolism and immune function underscores its relevance in human biological studies.

Bioss Inc.

300 Tradecenter Drive

Suite 4610

Woburn, MA 01801

United States

Phone: 800.501.7654 (Toll free)
Phone: +1.781.569.5821 (International)

Email: info@biossusa.com
orders@biossusa.com
support@biossusa.com
sales@biossusa.com

Company Profile

Website: www.biossusa.com

(1) Disruption of cholesterol homeostasis triggers periodontal inflammation and alveolar bone lossExperimental & molecular medicineDecember 1, 2023Tran TT, Lee G, Huh YH, Chung KH, Lee SY, Park KH, Kwon SH, Kook MS, Chun JS, Koh JT, et al.(Cayman, 160106; 1:150), rabbit anti-RORα (Sigma‒Aldrich, AV45608; 1:150), rabbit anti-CH25H (Bioss, bs-6480R; 1:150), and rabbit anti-CXCL16 (Genetex, GTX116706; 1:150), followed by staining with EnVision+System-HRP

(2) GPR183 antagonism reduces macrophage infiltration in influenza and SARS-CoV-2 infectionThe European respiratory journalMarch 1, 2023Foo CX, Bartlett S, Chew KY, Ngo MD, Bielefeldt-Ohmann H, Arachchige BJ, Matthews B, Reed S, Wang R, Smith C, et al.(40143-R040; Sino Biological), ionised calcium-binding adapter molecule 1 (IBA1) (019-19741; NovaChem), CH25H (BS-6480R; Bioss Antibodies), CYP7B1 (BS-5052R; Bioss Antibodies) and isotype control (rabbit IgG; 31235; Thermo Fisher Scientific

(3) An epithelial cell-derived metabolite tunes immunoglobulin A secretion by gut-resident plasma cellsNature ImmunologyMarch 1, 2023Simona Ceglia, Alyssa Berthelette, Kelsey Howley, Yun Li, Benedikt Mortzfeld, Shakti K. Bhattarai, Nicole K. H. Yiew, Ying Xu, Robert Brink, Jason G. Cyster, et al.resolved by SDS-PAGE and CH25H was quantified by immunoblotting with anti-CH25H (Bioss antibodies, #Bs6480R, used 1:500), followed by incubation with HRP-conjugated anti Rabbit antibody (Jackson Labs, #711–035-152)

(4) Liver x receptor alpha drives chemoresistance in response to side-chain hydroxycholesterols in triple negative breast cancerOncogeneApril 1, 2021Hutchinson SA, Websdale A, Cioccoloni G, Røberg-Larsen H, Lianto P, Kim B, Rose A, Soteriou C, Pramanik A, Wastall LM, et al.(Abcam, Cambridge, UK - #ab126785), CYP46A1 (Abcam, Cambridge, UK - #ab198889), CH25H (Bioss, MA, US - #bs6480R), LXRα (R&D System, Minneapolis, US - #PP-PPZ0412-00), LXRβ (Active Motif, Carslbad, US - #61177). Antibody

(5) Treating the Intestine with Oral ApoA-I Mimetic Tg6F Reduces Tumor Burden in Mouse Models of Metastatic Lung CancerScientific reportsJune 13, 2018Chattopadhyay A, Yang X, Mukherjee P, Sulaiman D, Fogelman HR, Grijalva V, Dubinett S, Wasler TC, Paul MK, Salehi-Rad R, et al.(Avanti Polar Lipids, catalog #330001 S) at a 1:50 dilution or anti-CH25H antibody (Bioss Antibodies, bs-6480R) at a dilution of 1:100. The slides were rinsed in PBST and the signals were amplified using VECTSTAIN

(6) Transgenic tomatoes expressing the 6F peptide and ezetimibe prevent diet-induced increases of IFN-β and cholesterol 25-hydroxylase in jejunumJournal of lipid researchAugust 1, 2017Mukherjee P, Hough G, Chattopadhyay A, Navab M, Fogelman HR, Meriwether D, Williams K, Bensinger S, Moller T, Faull KF, et al.(Waltham, MA, catalog no. PA5-20390). Antibody to CH25H was from Bioss Antibodies (Woburn, MA, catalog no. bs-6480R). Unless otherwise stated, all other materials were from sources previously described (14). Mice and

Be the First to Write a Review!

WB

CH25H Polyclonal Antibody

CH25H Polyclonal Antibody from Bioss Inc.

Description

Questions and Answers

Bioss Inc.

Citations (6)

Reviews

CH25H Polyclonal Antibody from Bioss Inc.

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map