Fig 1: Percentages of HSPC are progressively reduced in CHIP and MDS bone marrow (BM) snapshot data(A) Representative BM snapshot analysis of healthy, CHIP, and MDS. For CMP, GMP, and MEP fractions, cells were gated on lin-CD45dimCD34+CD38+ cells and further distinguished using CD123 and CD45RA. For HSC, MPP, and MLP fractions, cells were gated on lin-CD45dimCD34+CD38− cells and further distinguished using CD90 and CD45RA.(B) BM snapshots of healthy, CHIP, and MDS samples reveal a significantly reduced percentage of hematopoietic stem and progenitor cells (HSPC) in all lin- cells in MDS patients (p = 0.038, Kruskal-Wallis test followed by Dunn’s multiple comparison test). Data are represented as individual points of independent samples, lines indicate median.(C) The frequencies of CMP and GMP are significantly reduced in MDS BM (p = 0.0031 for CMP healthy vs. MDS, p = 0.0074 for GMP healthy vs. MDS, p = 0.017 for GMP CHIP vs. MDS, Kruskal-Wallis test followed by Dunn’s multiple comparison test). For all compartments, a trend toward reduced frequencies from healthy over CHIP to MDS can be observed. Data are represented as individual points of independent samples, lines indicate median.