E2F-1 Antibody AF4825 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemE2F-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $449.00Supplier Page View Company Product Page
Catalog NumberAF4825
NCBI Gene AliasesRBBP3, RBAP1, RBP3, E2F-1
ApplicationsImmunohistochemistry,Western Blot,ELISA Capture
IsotypeIgG
HostSheep
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse
Conjugate/TagUnconjugated
TargetE2F-1
Quantity100 ug (also 25 ug)
NCBI Full Gene NameE2F transcription factor 1
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human E2F-1, Val264-Phe437, Accession # Q01094
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human E2F-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human E2F-2, -3, -4, -5, -6, -7, and -8 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) A selective chemical probe for exploring the role of CDK8 and CDK19 in human diseaseNature chemical biologyDecember 1, 2015Dale T, Clarke PA, Esdar C, Waalboer D, Adeniji-Popoola O, Ortiz-Ruiz MJ, Mallinger A, Samant RS, Czodrowski P, Musil D, et al.together with commercially available antibodies against total E2F1 (sheep-anti-E2F-1, R&D Systems #AF4825 and rabbit-anti-E2F-1, Cell Signalling #3742) to quantify phospho-E2F1SER375 and total E2F1. Supplementary

(2) A Cre-conditional MYCN-driven neuroblastoma mouse model as an improved tool for preclinical studiesOncogeneJune 1, 2015Althoff K, Beckers A, Bell E, Nortmeyer M, Thor T, Sprüssel A, Lindner S, De Preter K, Florin A, Heukamp LC, et al.primary antibodies against Brd4 (1:200; #sc48772; Santa Cruz, Heidelberg, Germany), E2f1 (1:1000; #AF4825; R&D Systems, Minneapolis, MN, USA) or MYCN (1:1000; #9405; Cell Signaling, Frankfurt am Main, Germany)

(3) BET bromodomain protein inhibition is a therapeutic option for medulloblastomaOncotargetNovember 1, 2013Henssen A, Thor T, Odersky A, Heukamp L, El-Hindy N, Beckers A, Speleman F, Althoff K, Schäfers S, Schramm A, et al.sc-48772, Santa Cruz, Dallas, TX, USA), Cyclin D1 (1:200, H-295, sc-753, Santa Cruz), E2F1 (1:200, AF4825, R&D Systems, Minneapolis, MI, USA) and B-actin (1:2000, Sigma-Aldrich, St. Louis, MO, USA) as the loading

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E2F-1 Antibody

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E2F-1 Antibody

E2F-1 Antibody

E2F-1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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E2F-1 Antibody from R&D Systems, a Bio-Techne Brand

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