Fig 1: Differences in zonal abundance patterns of BSEP are associated with all histologic features of NAFLD, while zonal abundance patterns of NTCP and OATP1B3 are influenced by lobular inflammation. Mean marginal effects on relative zonal abundances of (A) BSEP, (B) NTCP, (C) OATP1B1, and (D) OATP1B3 were calculated by comparing biopsies of various histologic scores to a score of 0 with the corresponding histologic category. Median and 95% credible intervals of each posterior distribution are shown. The sample sizes for each histologic score are as follows: S0 (n = 10), S1 (n = 7), S2 (n = 8), S3 (n = 5); I0 (n = 10), I1 (n = 17), I2 (n = 3); B0 (n = 20), B1 (n = 7), B2 (n = 3); F0 (n = 25), F1-F2 (n = 5). *95% credible interval does not cross 0. Abbreviations: BSEP, bile salt export pump; NTCP, sodium-taurocholate cotransporting polypeptide; OATP, organic anion transporting polypeptide.
Fig 2: Relative zonal abundance patterns of BSEP and OATP1B3 are altered in NASH and NAFLD, respectively. (A) “Cellular Level” and “Hepatic Lobule” schematics created with BioRender.com depict hepatocyte transporters of interest on the basolateral (NTCP, OATP1B1, and OATP1B3) and canalicular (BSEP) plasma membrane domains. Na+/K+-ATPase (basolateral) and aminopeptidase N (CD13; canalicular) serve as domain-specific markers. “Tissue level” shows representative masked confocal fluorescence images of transporter zonal abundance. Scale bars, 100 μm. (B) Median (quartile 1; quartile 3) relative transporter abundance patterns along the PT-to-CV axis. Plotted are triplicate measures (where available) taken from different areas of each liver biopsy. (C) Mean marginal effect on relative zonal transporter abundances for NAFL and NASH biopsies compared to NDL. Median and 95% credible intervals of each posterior distribution are shown. *95% credible interval does not cross 0. Abbreviations: BA, bile acid; BSEP, bile salt export pump; CV, central vein; NDL, non-diseased liver; NTCP, sodium-taurocholate cotransporting polypeptide; OATP, organic anion transporting polypeptide; PT, portal tract.
Fig 3: Relative zonal abundance patterns of BSEP and OATP1B3 are altered in NASH and NAFLD, respectively. (A) “Cellular Level” and “Hepatic Lobule” schematics created with BioRender.com depict hepatocyte transporters of interest on the basolateral (NTCP, OATP1B1, and OATP1B3) and canalicular (BSEP) plasma membrane domains. Na+/K+-ATPase (basolateral) and aminopeptidase N (CD13; canalicular) serve as domain-specific markers. “Tissue level” shows representative masked confocal fluorescence images of transporter zonal abundance. Scale bars, 100 μm. (B) Median (quartile 1; quartile 3) relative transporter abundance patterns along the PT-to-CV axis. Plotted are triplicate measures (where available) taken from different areas of each liver biopsy. (C) Mean marginal effect on relative zonal transporter abundances for NAFL and NASH biopsies compared to NDL. Median and 95% credible intervals of each posterior distribution are shown. *95% credible interval does not cross 0. Abbreviations: BA, bile acid; BSEP, bile salt export pump; CV, central vein; NDL, non-diseased liver; NTCP, sodium-taurocholate cotransporting polypeptide; OATP, organic anion transporting polypeptide; PT, portal tract.
Fig 4: Differences in zonal abundance patterns of BSEP are associated with all histologic features of NAFLD, while zonal abundance patterns of NTCP and OATP1B3 are influenced by lobular inflammation. Mean marginal effects on relative zonal abundances of (A) BSEP, (B) NTCP, (C) OATP1B1, and (D) OATP1B3 were calculated by comparing biopsies of various histologic scores to a score of 0 with the corresponding histologic category. Median and 95% credible intervals of each posterior distribution are shown. The sample sizes for each histologic score are as follows: S0 (n = 10), S1 (n = 7), S2 (n = 8), S3 (n = 5); I0 (n = 10), I1 (n = 17), I2 (n = 3); B0 (n = 20), B1 (n = 7), B2 (n = 3); F0 (n = 25), F1-F2 (n = 5). *95% credible interval does not cross 0. Abbreviations: BSEP, bile salt export pump; NTCP, sodium-taurocholate cotransporting polypeptide; OATP, organic anion transporting polypeptide.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for OATP1B1/OATP2 Antibody (ESL)
Available conjugates: Available conjugates: UnconjugatedSpecificity: OATP2 (ESL)Sizes Available: 100uL (also 0.025 ml)