Anti-FT/TSF antibody - Flowering locus T and twin sister of FT AS06 198 from Agrisera

Product Specs
ItemAnti-FT/TSF antibody - Flowering locus T and twin sister of FT
CompanyAgrisera
Price EUR329.00Supplier Page View Company Product Page
Catalog NumberAS06 198
Quantity50 µg
ApplicationsWestern blot
FormatLyophilized
TargetAnti-FT/TSF
ReactivityArabidopsis thaliana, Oryza sativa
HostRabbit
ImmunogenKLH-conjugated synthetic peptide derived fromA.thaliana FT protein sequence(Q9SXZ2, At1g65480); please note that this antibody will cross-react with the highly homologous TSF (twin sister of FT) protein
ClonalityPolyclonal
NCBI Gene AliasesFLOWERING LOCUS T, F5I14.3, RSB8, F5I14_3, REDUCED STEM BRANCHING 8
Research AreaPlant Biology
PurityImmunogen affinity purified serum in PBS pH 7.4
StorageStore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
Molecular Weight20 : 20 kDa (Arabidopsis thaliana)

Specifications/Features

1 : 1000, detected with standard ECL (WB)

Questions and Answers

Can this antibody be used to study the role of FT in flowering time control in Arabidopsis?

Yes, the FT antibody has been utilized in research to assess FT protein levels in transgenic Arabidopsis lines, as demonstrated in the study by Nakamura et al. (2019). The antibody was employed in immunoblot assays to evaluate the expression of FT mutants, which are crucial for understanding the molecular mechanisms of flowering time regulation.

What specific applications has this antibody been shown to work in?

The FT antibody is specifically indicated for use in Western Blot applications. It has been effectively used to detect FT protein levels in various transgenic Arabidopsis lines, providing insights into the functional implications of FT in flowering processes.

Does this antibody cross-react with other proteins, and how might that affect my experiments?

Yes, the FT antibody is known to cross-react with the highly homologous TSF (twin sister of FT) protein. This cross-reactivity could be significant if your experiments aim to distinguish between the functions of FT and TSF, as it may complicate the interpretation of results if both proteins are present in your samples.

What is the significance of the phospholipid-binding site identified in FT, and how does this relate to the use of the antibody?

The identification of a phospholipid-binding site in FT, as reported by Nakamura et al. (2019), is crucial for understanding how FT modulates flowering time. The antibody can be used to investigate the expression and functionality of FT mutants that affect this binding, thereby providing valuable information on the biochemical pathways involved in flowering regulation.