Argonaute 2 (C34C6) Rabbit mAb 2897S from Cell Signaling Technology

Product Specs
ItemArgonaute 2 (C34C6) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number2897S
Quantity100 µl
ApplicationsW, IP
ReactivityH, M, R, Mk
TargetAgo2 mouse
HostRabbit
NCBI Gene Aliases1110029L17Rik, 2310051F07Rik, Eif2c2, Gerp95, Gm10365, mKIAA4215
ClonalityMonoclonal
NCBI Full Gene Nameargonaute RISC catalytic subunit 2
Research AreaTranslational Control
CloneC34C6
Molecular Weight97

Questions and Answers

Can this antibody be used for RNA immunoprecipitation studies?

Yes, the Argonaute 2 (C34C6) Rabbit mAb has been utilized in RNA immunoprecipitation assays, as demonstrated in studies where it was used to confirm interactions between long non-coding RNAs and microRNAs in various cancer cell lines, including U2OS and MG-63 cells .

What species can this antibody detect, and is it effective for human samples?

The Argonaute 2 (C34C6) Rabbit mAb is reactive with multiple species, including human, mouse, rat, and non-human primates, making it suitable for a wide range of biological studies .

Has this antibody been cited in research related to cancer?

Yes, the Argonaute 2 (C34C6) Rabbit mAb has been cited in numerous studies, including those investigating the role of long non-coding RNAs in cancer progression, such as in osteosarcoma and lung adenocarcinoma, where it was used to analyze the expression of key regulatory proteins .

What applications is this antibody suitable for in the context of protein detection?

The Argonaute 2 (C34C6) Rabbit mAb is suitable for applications such as Western blotting and immunoprecipitation, allowing for the detection of endogenous levels of Argonaute 2 protein in various experimental setups .

Cell Signaling Technology

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) LINC01929 mediates breast cancer immunosuppression and is an immunotherapy targetiScienceApril 17, 2026Jaganathan Venkatesh, Marie-Claire D. Wasson, Raj Pranap Arun, Hannah F. Cahill, Olivia L. Walker, Cheryl A. Dean, Wasundara Fernando, Maya R. MacLean, Meghan E. McLean, Bakhmala Khan, et al.were incubated with 1/1000 anti-PSMB8 (LMP7, cat# ab180606, Abcam, Cambridge, UK) or anti-AGO2 (cat #2897, Cell Signaling Technology, Danvers, MA, USA) diluted in 5% bovine serum albumin, overnight at 4 °C

(2) Single-cell transcriptomic analysis reveals that the circRNA circGCLM promotes tumorigenesis and confers cisplatin resistance in NSCLC through the miR-505-3p/ERBB4 axisTransl OncolApril 7, 2026Yirou Ma, Baiqing Huang, Haiyang Wang, Mengchao Xue, Yiyang Liu, Yibo Jin, Chunping Wang, Shaorui Gu, Yongxin Zhoubuffer and incubated overnight at 4 °C with magnetic beads pre-bound to 5 µg of anti‑Ago2 (CST, USA, 2897) or control IgG. Following extensive washing, immunoprecipitated complexes were digested with Proteinase

(3) Male obesity causes adipose mitochondrial dysfunction in F1 mouse progeny via a let-7-DICER axisNature CommunicationsFebruary 24, 2026Chien Huang, Joo-Hyun Park, Ali Altıntaş, Natasa Stanic, Kristine Kyle de Leon, Signe Isacson, Panagiotis Kalogeropoulos, Hande Topel, Tobias Madsen, Sebastian Zanner, et al.dilution), VDAC (Cell Signaling Technologies #4661, 1:11,000 dilution), HK2 (Cell Signaling Technologies #2897, 1:1,1000 dilution), SLC25A24 (Abcam Ab221120, 1:1000 dilution), CPT2 (Abcam Ab181114, 1:1,1000 dilution),

(4) Long noncoding RNA GAS5 acts as a competitive endogenous RNA to regulate GSK-3β and PTEN expression by sponging miR-23b-3p in Alzheimer’s diseaseNeural Regeneration ResearchJanuary 1, 2026Li Zeng, Kaiyue Zhao, Jianghong Liu, Mimin Liu, Zhongdi Cai, Ting Sun, Zhuorong Li, Rui Liumagnetic beads coupled with anti-Argonaute 2 antibody (Cell Signaling Technology, Danvers, MA, USA, Cat# 2897, RRID: AB_2096291) or anti-IgG overnight at 4°C. The protein–RNA complex was then treated with proteinase

(5) Stress-Associated Changes in MiR-20b-3p as a Potential Predictor of Underlying Psychopathology in the Depressed Brain: Mechanistic Insights from a Rat Model of Chronic Restraint StressMolecular NeurobiologyJanuary 1, 2026Sarah Ali, Yogesh Dwivedirecombinant Argonaute-2 (Ago2, cat # C34C6) rabbit monoclonal antibody (Cell Signaling Technology, cat # 2897S) and incubated overnight with the clarified tissue and cellular lysates. Afterward, the RNA was isolated

(6) Transcription factor FOXC2 regulates miR-145/ADAMTS5 axis to inhibit angiogenesis in hepatocellular carcinoma via circular RNA 0002898BMC CancerNovember 25, 2025Jiangbei Yuan, Zixiang Pan, Fei Lv, Yue Zhao, Wei Zheng, Qiaoqiao Yin, LanJie Wu, Jianli Yu, Cheng’an Xu, Qiang He, et al.and RNase inhibitors. The lysate was incubated with magnetic beads conjugated with AGO2 (CST, USA, 2897) or IgG (CST, USA, 2729 S) antibodies at 4 °C. After washing, proteinase K treatment was applied to

(7) SARS-CoV-2-ORF3a variant Q57H reduces its pro-apoptotic activity in host cellsF1000ResearchNovember 22, 2025Maria Landherr, Iuliia Polina, Michael W. Cypress, Brian Rhee, Kye-Im Jeon, Sanjana Chandran, Isabel Chaput, Bridget Nieto, Young Min Bae, Bong Sook Jhun, et al.peptide NWSHPQFEK conjugated to KLH Argonaute 2 (Argo2),Rabbit monoclonal,Cell Signaling Technology,"2897S, AB_2096291",Synthetic peptide corresponding to mouse argonaute 2 Cytochrome C,Mouse monoclonal,BD Bioscience

(8) Argonaute 1 contributes to the transcriptional silencing of HIV-1Journal of Biological ChemistryOctober 1, 2025Sophie Goudey, Melissa Ait Ait Said, Juliette Desforges, Sébastien Marie, Marina Morel, Clarisse Berlioz-Torrent, Florence Margottin-Goguet, Stéphane Emiliani, Roy Matkovic, Sarah Gallois-Montbrunantibodies: anti-Ago1 4B8 (SAB4200084; Sigma), anti-Ago1 D84G10 (5053; Cell Signaling), anti-Ago2 C34C6 (2897; Cell Signaling), anti-Dicer A-2 (sc-136981; Santa Cruz), anti-Drosha D28B1 (3364; Cell Signaling),

(9) Radiation-sensitive circRNA promotes intestinal regenerationCell Mol Biol LettAugust 29, 2025Hui Cai, Xin Liang, Shazhen Ai, Hao Sun, Xinyu Zhang, Qianying Lu, Qingshan Yang, Ying Li, Di Zhao, Manman Zhang, et al.RNase inhibitors. The cell lysates were incubated with magnetic beads conjugated with AGO2 antibodies (2897S, CST) or IgG (12–371, Millipore) at 4 °C overnight. Then, the beads were washed and incubated with proteinase

(10) Alpha-synuclein abundance and localization are regulated by the RNA-binding protein PUMILIO1Cell ReportsAugust 26, 2025Maximilian Cabaj, Pietro G. Mazzara, Zachary A. Gaertner, Ruizhi Wang, Michaela M. Pauers, Lisa K. Randolph, Cláudio Gouveia Roque, Sean Feeney, Serena Raimo, Nicola de Prisco, et al.Purified",Bethyl,Cat# A300-201A RRID:AB_2253218 Rabbit Anti-Argonaute 2 Monoclonal,Cell Signaling Technology,Cat# 2897 RRID:AB_2096291 Recombinant Anti-Alpha-synuclein,Abcam,Cat# ab212184 RRID:AB_2941889 Mouse Anti-Rabbit

(11) C-terminal tagging impairs AGO2 functionRNA BiolJuly 23, 2025Kunal M. Shah, Alex F. F. Crozier, Anika Assaraf, Muzjda Arya, Paul Grevitt, Faraz Mardakheh, Michael J. Plevin, Tyson V. Sharpmouse anti-Vinculin (Invitrogen, 14–9777–82), rabbit anti-AGO1 (CST, 5053), rabbit anti-AGO2 (CST, 2897), rabbit anti-AGO2 (Sino Biological 101,620-T36), rabbit anti-AGO3 (Abcam ab154884), rabbit anti-AGO4

(12) Long Non‐Coding RNA IGFRIL Couples with PTBP1 to Destabilize IGFBP3 mRNA to Promote the IGF1R‐AKT‐mTOR Axis and Hepatocellular CarcinomaAdvanced ScienceJuly 21, 2025Jing Zhang, Chengming Gao, Haibei Li, Yuying Chen, Liting Yang, Qian Jin, Lan Feng, Xia Wang, Xinyi Liu, Hongxia Chen, et al.Magna RIP RNA‐Binding Protein Immunoprecipitation kit (Millipore, USA). Antibody against PTBP1 (32‐4800; Thermo Fisher, USA) or against AGO2 (Cat. 2897; CST, USA) was used for the RIP. Cells were cultured in a 10‐cm plate, and then washed twice using 10

(13) MicroRNA miR-193b-3p Regulates Esophageal Cancer Progression Through Targeting RSF1CellsJune 19, 2025Yao Lin, Xudong Zhao, Zhenhua Du, Zhili Jia, Siyu Zhou, Gengsheng Cao, Hengbin Wangsupernatants were incubated overnight with RIP buffer containing either anti-Ago2 antibody (CST, Danvers, MA 2897T, US) or beads conjugated to anti-IgG as a negative control. After purifying the immunoprecipitated RNA

(14) MicroRNA mechanisms instructing Purkinje cell specificationNeuronMay 21, 2025Norjin Zolboot, Yao Xiao, Jessica X. Du, Marwan M. Ghanem, Su Yeun Choi, Miranda J. Junn, Federico Zampa, Zeyi Huang, Ian J. MacRae, Giordano LippiTechnology,Cat#D84G10; RRID:AB_2616013 Rabbit anti-AGO2 for western blot,Cell Signaling Technology,Cat#2897S; RRID:AB_2096291 Rabbit anti-HA,Cell Signaling Technology,Cat#C29F4; RRID:AB_1549585 Rabbit anti-KCC2,Abcam,Cat#

(15) Exosomal circ_0001583 Drives Glioblastoma Cell Advancement Through the miR-647/CKAP2L PathwayMolecular NeurobiologyApril 15, 2025Yuhao Zhang, Shiming Liu, Cheng Wu, Xin Gao, Hongtao Zhao, Ou Li, Kaichuang Yang, Faliang Gaoovernight at 4 °C with 5 μg of anti-Ago2 antibody (Cell Signaling Technology, Danvers, MA, USA, Cat#2897, clone C34C6) or normal mouse IgG (Cell Signaling Technology, Cat#5415) pre-coupled to Protein A/G magnetic

(16) SFTSV NSs interacts with AGO2 to regulate the RNAi pathway for viral replicationJournal of VirologyApril 15, 2025Nasir Javaid, Tae-Won Jang, Yuting Fu, Younho Choiantibodies included DICER (clone D38E7, Cell Signaling [5362]), AGO2 (clone C34C6, Cell Signaling [2897]), GFP (clone B-2, Santa Cruz [sc-9996]; clone D5.1, Cell Signaling [2956]), β-actin (clone C4, Santa Cruz

(17) lncRNA HIF1A-AS2 acts as an oncogene to regulate malignant phenotypes in cervical cancerFrontiers in OncologyFebruary 27, 2025Yang Liu, Yunyan Zhang, Cha Chen, Bhaskar Roy, Qun Li, Wei Zhang, Xuan Zhang, Jieying Pu, Yuguang Li, Yanli Liu, et al.lysed, and the resulting lysis solutions were subsequently incubated with an antibody against Ago2 (CST, 2897S, USA) or an isotype control IgG. The RNA-protein complexes were immunoprecipitated with protein A agarose beads

(18) Identification of antiviral RNAi regulators, ILF3/DHX9, recruit at ZIKV stem loop B to protect against ZIKV induced microcephalyNat CommunFebruary 26, 2025Zhiwei Lei, Yu Gu, Ying Liu, Hailiang Liu, Xiaohua Lu, Weijie Chen, Lu Zhou, Pan Pan, Zhuohong Chen, Zhaoyang Yue, et al.overnight with magnet beads which were conjugated with IgG or AGO2 antibody (CST, AGO2 C34C6, Cat #2897). The antibody has been validated in the IP experiment37,72–74. The beads were washed with RIP buffer:

(19) circNR3C2 promotes chondrogenic differentiation and cartilage repair of human adipose‐derived stem cells via the hsa‐miR‐647/SOX9 pathwayAnimal Models and Experimental MedicineFebruary 13, 2025Dabiao Hou, Huajun Wang, Hao Guo, Dongbin Luo, Xiaofei Zheng, Simin Luobiotin‐coupled miRNA capture RIP assays were performed using Ago2 antibody (Argonaute 2 (C34C6) Rabbit mAb #2897, Cell Signaling Technology, Beverly, MA, USA) and Magna RIP RNA‐Binding Protein Immunoprecipitation Kit

(20) CircUCK2(2,3) promotes cancer progression and enhances synergistic cytotoxicity of lenvatinib with EGFR inhibitors via activating CNIH4-TGFα-EGFR signalingCell Mol Biol LettJanuary 30, 2025Xindong Wei, Anfeng Si, Shuai Zhao, Yi Fu, Jilei Li, Kedeerya Aishanjiang, Yujie Ma, Chang Yu, Bo Yu, Chunhong Cui, et al.transferred to a clean tube as input. Magnetic beads were pre-incubated with the anti-AGO2 antibody (CST #2897) or isotype control (CST #3900) for 1 h at room temperature, and then incubated with cell lysates at

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Argonaute 2 (C34C6) Rabbit mAb

Argonaute 2 (C34C6) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Argonaute 2 (C34C6) Rabbit mAb from Cell Signaling Technology

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