VEGF Receptor 2 (55B11) Rabbit mAb 2479L from Cell Signaling Technology

Product Specs
ItemVEGF Receptor 2 (55B11) Rabbit mAb
CompanyCell Signaling Technology
Price $816.00 Inquire
Catalog Number2479L
Quantity300 µl
ApplicationsW, IP, IHC-P, IF-F, IF-IC
ReactivityH, M
TargetVEGFR2
HostRabbit
NCBI Gene AliasesCD309, FLK1, VEGFR, VEGFR2
ClonalityMonoclonal
NCBI Full Gene Namekinase insert domain receptor
Research AreaRTK
IsotypeIgG
Clone55B11
Molecular Weight210, 230

Questions and Answers

Can this antibody be used to study the role of VEGFR2 in angiogenesis?

Yes, the VEGF Receptor 2 (55B11) Rabbit Monoclonal Antibody has been utilized in studies investigating the mechanisms of angiogenesis. For instance, it was employed in research demonstrating that VEGFR2 activation is crucial for endothelial cell elongation and migration during sprouting angiogenesis, as detailed in the study by Cao et al. ().

Is this antibody effective for immunoprecipitation experiments?

Yes, the VEGF Receptor 2 antibody has been shown to be effective in immunoprecipitation experiments. It was used in a study where it successfully precipitated VEGFR2 from tumor cell lysates, allowing for further analysis of its signaling pathways ().

What applications has this antibody been validated for in published research?

The VEGF Receptor 2 antibody has been validated for several applications, including Western blotting, immunocytochemistry, immunofluorescence, immunohistochemistry, and immunoprecipitation. It has been cited in numerous studies across these applications, indicating its versatility in various experimental contexts (, ).

Does this antibody show specificity for VEGFR2 in human and mouse samples?

Yes, the VEGF Receptor 2 (55B11) Rabbit Monoclonal Antibody is specific for VEGFR2 and does not cross-react with other family members. It has been shown to effectively detect endogenous levels of VEGFR2 protein in both human and mouse samples, as indicated by its use in various studies involving these species ().

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Tumor-like proliferation of CCM3 knockout endothelial cells: insights from semaxinib treatment and transcriptome profiling of co-culturesActa Neuropathol CommunApril 20, 2026Valeriia V. Saenko, Janne L. Feldmann, Dariush Skowronek, Debora Singer, Ole J. Schamuhn, Doreen Biedenweg, Sander Bekeschus, Ute Felbor, Matthias Rath, Robin A. PilzX-100 in PBS for 15 min prior to blocking, and then incubated with primary antibodies against VEGFR2 (#2479S, 1:200, 55B11, Cell Signaling Technology) or p-VEGFR2 (Tyr1175) (#2478S, 1:100, Cell Signaling Technology).

(2) Single-cell transcriptomic analyses reveal angiogenic vascular responses to chronic cerebral hypoperfusioniScienceApril 17, 2026Jiajing Shan, Ruyu Shi, Liyuan Jiang, Connor Dufort, Wanying Miao, Ting-Wei Mai, Junxuan Lyu, Julieta Barreiro, Kong Chen, Rehana K. Leak, et al.Antibody,Proteintech,Cat# 81323-2-RR; RRID: AB_3086457 Anti-VEGFR2 Antibody,Cell Signaling Technology,Cat# 2479T; RRID: AB_2212507 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant

(3) Development of striated muscle microvasculature across the perinatal period in lambsThe Journal of PhysiologyMarch 21, 2026Eileen I. Chang, Saif I. Al‐Juboori, Tristan B. Dear, Sreya Varanasi, Collin S. Painter, Holland E. Stacey, Emma F. Saunders, Jane Stremming, Samantha Louey, Dana Strode, et al.sc-271789, Santa Cruz Biotechnology Inc, Dallas, TX, RRID: AB_10710391), or anti-VEGF Receptor 2 (1:1000; #2479, Cell Signaling Technologies, RRID: AB_2212507) in 5% BSA in TBST. Membranes were then washed and incubated

(4) Vascular Endothelial Growth Factor-D Improves Lung Vascular Integrity During Acute Lung InjuryCirc ResMarch 13, 2026Yongdae Yoon, Lokesh Sharma, Wenwen Tang, Shannon Kirk, Micha Sam Brickman Raredon, Farida Ahangari, Johad Khoury, Hong Qian, Yunbo Ke, Mohan Tulapurkar, et al.such as IκBα (#4814), NF-κB p65 (#4764), Thr18/Ser19 Phospho-Myosin Light Chain 2 (#3674), VEGFR2 (#2479), Tyr951 Phospho-VEGFR2 (#2471), and Tyr1175 Phospho-VEGFR2 (#2478) from Cell Signaling Technology (Danvers);

(5) SARS-CoV-2 spike S1-mediated HIF-2α activation in retinal endothelial cells suggests a mechanism contributing to post-COVID endothelial dysfunctionFront ImmunolMarch 9, 2026Andrea Ribeiro, Timon Wallraven, Maciej Lech, Kristina Adorjan, Hans Christian Stubbe, Martina Seifert, Anna Wöhnl, Veronika Kesseler, Johanna Negele, Christoph SchmadererVEGFR2, claudin-5, and VE-cadherin (all from Cell Signaling; Cat. No. 8242, 36169, 71565, 44060, 73015, 2479, 49564, 2500, respectively), or PDK-1 (Abcam, Cat. No. ab202468) in an antibody dilution buffer containing

(6) Vasculogenic Mimicry: A Potential Therapeutic Target for Chondrosarcoma TherapyCellsFebruary 24, 2026Vincenzo Ingangi, Roberta Gatti, Gioconda Di Carluccio, Vincenzo Di Vaia, Margherita Cerrone, Gerardo Ferrara, Sara Scala, Maurizio Maddalena, Michele Gallo, Flavio Fazioli, et al.anti-VEGF Receptor 2 (1:1000 dilution, Rabbit mAb 55B11, Cell Signaling Technology, Danvers, MA, USA, #2479) overnight at 4 °C or 0.2 μg/mL anti-GAPDH (Santa Cruz Biotechnology, Dallas, TX, USA, sc-47724) at

(7) SHP2 regulates VEGFR2 Y1175/PLCγ signaling to impair tumor endothelial barrier stabilityiScienceFebruary 20, 2026Polina Kremmyda, Sara Owad, Sagnik Pal, Elvira Wildheim, Catarina Chanoca, Cecilia Lindskog, Elin Sjöberg(Tyr1175),Cell Signaling Technology,2478S; RRID: AB_331377 Rabbit anti-VEGFR2,Cell Signaling Technology,2479S; RRID: AB_2212507 Mouse anti-Yes,BD Biosciences,610376; RRID: AB_397759 Mouse anti-3-nitrotyrosine,Abcam,ab61392;

(8) Cytoplasmic LIM domain only 2 enhances tumor endothelial cell migration through integrin β1-mediated focal adhesion signalingActa Neuropathologica CommunicationsFebruary 4, 2026Min Ji Park, Junseok Jang, Cheol Gyu Park, Minseo Gwak, Jong-Whi Park, Hyunggee KimKorea), anti-p-VEGFR2 (Y1175; 1:1 000) (2478S; Cell Signaling, Danvers, MA, USA), anti-VEGFR2 (1:500) (2479S; Cell Signaling Technology, USA), anti-HIF1α (1:1 000) (ab51608; Abcam, Cambridge, England), anti-ITGA5

(9) Extracellular Vesicles (EVs) Derived from Senescent Endothelial Cells Promote Platelet ActivationInternational Journal of Molecular SciencesJanuary 15, 2026Whitney Venturini, Angel Cayo, Gabriel Diaz-Serrano, Sofia Sanhueza, Ricardo Huilcaman, Diego Méndez, Danitza Rebolledo-Mira, Catalina Silva-Pereira, Francisca Torres-Orellana, Felipe Troncoso, et al.TBS as indicated: anti-KDR (55B11) Rabbit mAb (Cell Signaling Technology, Danvers, MA, USA, Cat. No. 2479; 1:1000), anti-peNOS (BD Biosciences, San Jose, CA, USA, Cat. No. 612392), 1:1000), anti-eNOS (BD Biosciences,

(10) Analysis of high glucose injury using human induced pluripotent stem cell-derived kidney organoidsBMC NephrologyJanuary 8, 2026Zuping Qian, Die Yang, Yongli Li, Rong Dong, Jingjing Da, Yong Chen, Yan ZhaS" SALL1,Invitrogen,PA62057 SIX2,Cell Signaling,"18,596 S" KDR (VEGF Receptor 2),Cell Signaling,2479 S Nephrin,Invitrogen,PA5-109310 AQP1,Invitrogen,MA5-25401 PDGFR-β,Hangzhou Huaan Biotechnology,SY10-08 CDH1,Invitrogen,14-3249-82 CD31,abcam,Ab9498 IL-6,Affinity,DF6087 IL-1β,Affinity,AF5103 TGF-β1,BOSTER,MA00019 Vimentin,Invitrogen,PA27231 COL4,Invitrogen,14-9871-80 KIM-1,Invitrogen,PA5-79345 ADRP,Proteintech,15294-1AP IL-10,Immunoway,YM8444 TNF-α,Immunoway,YM8306 Fibronectin,Immunoway,YM8309 WT1,Invitrogen,MA5-3225 "Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed

(11) CD146 + pericyte-like lung cancer brain metastatic stem cells promote tumor angiogenesis through dual regulatory effects on the VEGF/VEGFR axisTheranosticsJanuary 1, 2026Wenwen Liu, Wenzhe Duan, Shengkai Xia, Ye Liu, Huiying Chu, Kun Liang, Shaobo Fang, Manqing Lin, Song Wei, Xin You, et al.VEGFB (ab133606, Abcam, RRID:AB_3698545), VEGFC (67116-1-IP, Proteintech, RRID:AB_2882420), VEGFR2 (2479, Cell Signaling Technology, RRID:AB_2212507; A16985H, BioLegend, RRID:AB_2728423), phospho-VEGFR2 (3370,

(12) Prickle4 Drives Microenvironmental Remodeling and Resistance to Parp Inhibition in IDH‐Mutant GliomaAdvanced ScienceJanuary 1, 2026Ju Yang, Hua Yang, Yifan Yuan, Chenyang Zhang, Ziwei Fu, Yanyan Chen, Yinghong Xiong, Shuyu Chen, Kexin Ling, Ying Liu, et al.phospho‐VEGFR2 Tyr 1175 (Cell Signaling Technology, 2478s, 1:1000); total VEGFR2 (Cell Signaling Technology, 2479s, 1:1000), HA‐tag (Cell Signaling Technology, 5017, 1:1000); Anti‐FLAG (Cell Signaling Technology, 14793,

(13) Protocol for generation of endothelial cells from human-derived iPSCs and integration into mouse brain explantsSTAR ProtocDecember 5, 2025Iza Erzar, Nora Noll, Roberta Lugano, Anna Dimberg, Maximiliano Arce, Peetra U. Magnussondilution),Abcam,ab92513; RRID: AB_2630401 Rabbit anti-VEGFR2 (1:100 dilution),Cell Signaling Technology,2479; RRID: AB_2212507 Mouse anti-P120 (1:100 dilution),BD Biosciences,610134; RRID: AB_397537 Rabbit anti-ETV2

(14) Ginsenoside Re regulates PFKFB3-mediated glycolysis to inhibit endothelial cell migration to ameliorate atherosclerosisJournal of Ginseng ResearchDecember 4, 2025Zhihui Wang, Junyu Mou, Wen Han, Siyuan Liu, Min Wang, Guibo SunCell Signaling Technology, Inc.); VEGFA Rabbit mAb (Cat. ab214424, abcam); VEGFR2 Rabbit mAb (Cat. 2479, Cell Signaling Technology, Inc.). 2.2 Animals and experimental design Six-week-old male ApoE−/− mice

(15) Farrerol inhibits proliferation and migration of colorectal cancer via the VEGF signaling pathway: evidence from network pharmacology, molecular docking, molecular dynamics simulation, and in vitro experimentsFront PharmacolDecember 3, 2025Longhui Zhang, Qijing Xu, Guanduo Sun, Xiaokang Zhang, Jialong Xue, Chun Yao, Dechun Liu, Jingming ZhaiAnti-GAPDH (catalog number. 60004-1-Ig) was purchased from Proteintech Group, Inc. VEGFA (#2478), VEGFR2 (#2479), and p-VEGFR2 (#50661) were purchased from Cell Signaling Technology. 2.11 Cell cuture and treatment HCT116 cells

(16) Slow-twitch oxidative myofiber proportions and capillary number per myofiber are reduced in growth-restricted fetal sheepAmerican Journal of Physiology-Regulatory, Integrative and Comparative PhysiologyDecember 1, 2025Tristan B. Dear, Eileen I. Chang, Saif I. Al-Juboori, Jane Stremming, Stephanie R. Wesolowski, Sonnet S. Jonker, Paul J. Rozance, Evgenia Dobrinshkikh, Laura D. Brown(1:1000; #PA1–16948, Invitrogen, RRID: AB_2288261) in 5% BSA in TBST or anti-VEGF Receptor 2 (1:1000; #2479, Cell Signaling Technologies, RRID: AB_2212507) in 5% BSA in TBST. After incubation overnight at 4 °C,

(17) Deficient chaperone-mediated autophagy in macrophages aggravates colitis and colitis-associated tumorigenesis in miceMolecules and CellsDecember 1, 2025Weichun Zhu, Zehao Chen, Yunqian Gao, Chungang Zhai, Xia Li, Ning Wang, Kang Fu, Wentao Chen, Jieqiong Peng, Dan Xu, et al.(ab16667, Abcam), HIF-1α (YT2133, Immunoway), IL-1β (ab283818, Abcam), VEGFA (ab52917, Abcam), and VEGFR2 (2479, CST). The following day, sections were brought to room temperature for 30 min, washed three times with

(18) Discovery of a Proteolysis Targeting Chimera for TRKA and RET-derived oncoproteinsScientific ReportsNovember 25, 2025Marialuisa Moccia, Lingzhi Zhang, Zhengyu Wang, Minmin Wang, Giorgia Federico, Massimo Santoro, Hong-yu Li, Francesca Carlomagno(Thermo Fisher Scientific) and films were developed using an X-ray film developer. Antibodies for VEGFR2 (2479), phospho-TRKA (Tyr490) (9141), phospho-TRKA (Tyr674/675) (4621), panTRK (A7H6R) (92991) and RET (14698)

(19) Engineering a heparin-mimetic biomaterial to promote tissue vascularizationCommunications BiologyNovember 14, 2025Linqing Li, Jinling Yang, Luba Perry, Jennifer L. Bays, Sangeeta N. Bhatia, Jeroen Eyckmans, Christopher S. Chenand subjected to Western blot analysis using antibodies from Cell Signaling (pVEGFR2, 2478; VEGFR2, 2479; pERK1/2, #4370; ERK1/2, #4695; pAkt, #9271; Akt, #9272; and GAPDH, #5174). The blots were developed

(20) APOA1 binding protein promotes lymphatic cell fate and lymphangiogenesis by relieving caveolae-mediated inhibition of VEGFR3 signalingNat CommunOctober 21, 2025Jun-Dae Kim, Surbhi Chaudhary, Weiqing Chen, Jonathan Astin, Philip S. Crosier, Pengchun Yu, John P. Cooke, Henry J. Pownall, Hugo J. Bellen, Nhat-Tu Le, et al.#3238S; 1:1000), Flotillin-1 (Abcam #ab41927; 1:500), GFP (Invitrogen #MA1-952; 1:1000), VEGFR2 (CST #2479L; 1:1000), pY1175-VEGFR2 (CST #2478S; 1:1000), 4G10 (CST #96215S; 1:1000), GRB2 (CST #3972S; 1:1000),

Write a Review

(1)

6/3/2016IHC Analysis Anil KumarThis antibody was used to analyze VEGFR2 protein expression in prostate cancer xenograft tissue. This antibody gave best results at 1:150 dilution in PBS for overnight incubation. This antibody was ... Read Review

VEGF Receptor 2 (55B11) Rabbit mAb

VEGF Receptor 2 (55B11) Rabbit mAb from Cell Signaling Technology

Description

Questions and Answers

Cell Signaling Technology

Citations (426)

Reviews

VEGF Receptor 2 (55B11) Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map