Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb 9323S from Cell Signaling Technology

Product Specs
ItemPhospho-GSK-3beta (Ser9) (5B3) Rabbit mAb
CompanyCell Signaling Technology
Price $401.00 Inquire
Catalog Number9323S
Quantity100 µl
ApplicationsW, IHC-P, IF-IC
ReactivityH, M, R, Mk
TargetGSK3-beta (Ser9) phosphate
ModificationsGSK3-beta (Ser9) phosphate
HostRabbit
ClonalityMonoclonal
NCBI Full Gene Nameglycogen synthase kinase 3 beta
Research AreaPI3K / Akt Signaling
Clone5B3
Molecular Weight46

Questions and Answers

Can this antibody be used to study GSK-3β phosphorylation in different species?

Yes, the Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb is reactive with multiple species, including human, mouse, rat, and non-human primates, making it suitable for comparative studies across these organisms.

Has this antibody been utilized in any specific research contexts, particularly in cancer studies?

Yes, this antibody has been cited in various studies, including research on breast cancer where it was used to assess the phosphorylation status of GSK-3β, which is implicated in the regulation of cell survival and proliferation pathways in cancer cells ().

What applications is this antibody suitable for in laboratory experiments?

The Phospho-GSK-3β (Ser9) (5B3) Rabbit mAb has been shown to work effectively in several applications, including Western blotting, immunocytochemistry, immunofluorescence, and immunohistochemistry, allowing for versatile usage in protein detection and localization studies.

Are there any notable findings related to GSK-3β that this antibody has helped uncover in neurobiology?

Yes, studies have demonstrated that inhibition of GSK-3β, which can be monitored using this antibody, plays a significant role in neurogenesis and the response to ischemic conditions, highlighting its importance in neurodegenerative research ().

Cell Signaling Technology

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) A longitudinal, multi-omic atlas reveals the emergence of a spatially organized immunosuppressive ecosystem in resistant melanomaCell Reports MedicineApril 21, 2026Shiyou Wei, Yulan Deng, Jinho Lee, Hongbin Lan, Zhenyu Yang, Marilyne Labrie, Courtney B. Betts, Hao Duan, Benjamin Tate, Joanna Pucilowska, et al.RRID:AB_10695860 PDGFR,R&D,Cat# AF385; RRID:AB_355339 KI67,CST,Cat# 12075S; RRID:AB_2728830 pGSK3b,CST,Cat# 9323S; RRID:AB_2115201 CD68,Biolegend,Cat# 916104; RRID:AB_2616797 pEGFR,R&D,Cat# AF1095; RRID:AB_416526 CD45,abcam,Cat#

(2) 4-Methylesculetin Ameliorates Hepatic Insulin Resistance in HepG2 Cells Through AMPK/FOXO1, PI3K/AKT/GSK3β Pathways and SIRT1/NOX4 AxisJ Diabetes ResApril 20, 2026Xiaohua Su, Yuhang Du, Yang Yang, Yige Zhao, Hongbin Zhao, Jiamei Xie, Ziyi Shan, Menglu Wang, Zhiyun Huang, Wanxin Fu, et al.anti‐AKT (1:1000, ab179463, Abcam), antiphosphorylated glycogen synthase kinase‐3β (p‐GSK3β) (1:1000, 9323, CST), antiglycogen synthase kinase‐3β (GSK3β) (1:1000, ab280376, Abcam), anti‐G‐6pase (1:1000, ab31905,

(3) A Standard Herbal Formula, CGAC, Attenuates Bone Loss by Normalizing Low-Bone Turnover Stagnation in an Orchiectomy-Induced Mouse ModelPharmaceuticals (Basel)March 31, 2026Dong-Cheol Baek, Min-Young Chae, Tae-Wook Woo, Chang-Gue Son, Eun-Jung LeeCell Signaling Technology), β-catenin (1:1000, ab32572, Abcam, Cambridge, UK), phospho-GSK3β (Ser9) (1:1000, #9323S, Cell Signaling Technology), GSK3β (1:1000, #9315S, Cell Signaling Technology), PPAR-γ (1:1000, #2443S,

(4) Trefoil Factor-3 Is a Hypoxia-Triggered Pro-Tumorigenic Factor in HepatoblastomaLiver IntMarch 13, 2026Luz A. Martínez-Pérez, M. Ujue Latasa, Iker Uriarte, Amaya López-Pascual, Jasmin Elurbide, Pavel Strnad, Sona Frankova, Eva Sticova, Ondrej Fabian, Maria Arechederra, et al.all from Abcam (Cambridge, UK). Anti‐CD31 (77 699; 1:200), anti‐HIF1α (48 085; 1:50), anti p‐GSK‐3β (9323; 1:50), anti‐p‐S6RP (4858; 1:200) and anti‐GLUT1 (73 015; 1:250) were from Cell Signalling Technology

(5) Wnt-3a exacerbates production of TNF-α in LPS stimulated microglia independent of the β-catenin canonical pathwayScientific ReportsFebruary 11, 2026Gabrielle Federici, Sandy Stayte, Peggy Rentsch, Bryce Vissel(Ser33/37/Thr41) (Cell Signaling, cat # 4270 s at 1:10), phosphorylated-GSK-3β (S9) (Cell Signaling, cat # 9323 s at 1:25), phosphorylated-GSK-3β (Tyr216) (Abcam, cat # Ab75745 at 1:10), NFκB p65 (Cell Signaling,

(6) O-GlcNAcylation expression predicts a favorable prognosis and mitigates malignant phenotypes via MYCN suppression in neuroblastomaMol Cell PediatrFebruary 10, 2026Neng-Yu Lin, Hsiu-Hao Chang, Chia-Yeh Hsieh, Hsiu-Ling Chang, Wan-Ling Ho, Yen-Lin Liu, Pei-Yi Wu, Chi-Tai Yeh, Min-Chuan Huang, Wen-Ming HsuGeneTex, [1:5000]), GSK3β (Cat. No. GTX133372, GeneTex, [1:1000]), Phospho-GSK-3β (Ser9) (Cat. No. 9323T, Cell Signaling, [1:1000]), Phospho-c-Myc (Thr58) (Cat. No. 46650T, Cell Signaling, [1:1000]) and MYCN

(7) Transglutaminase 2 exacerbates ovarian cancer survival by directly inactivating GSK3βCell Death DisFebruary 2, 2026Ho Lee, Joon Hee Kang, Hyun Jung Kim, Kyun Heo, Mi Kyung Park, Jeong Hwan Park, Byung Il Lee, Jong In Yook, Soo-Youl Kim(#sc-7392) were from Santa Cruz Biotechnology (Dallas, TX, USA); anti-GSK3β (#12456), anti-p-GSK3β (Ser9) (#9323), anti-non-p (Active) β-catenin (Ser33/37/Thr41) (#8814) and anti-Snail (#3879) were from Cell Signaling Technology

(8) Single-cell analysis identifies BASP1 as a driver of drug resistance and cell plasticity in oral squamous cell carcinomaJournal of Biological ChemistryFebruary 1, 2026Abinash Behera, Sudeshna Datta, Sibasish Mohanty, Pallavi Mohapatra, Shamima Azma Ansari, Sreeparna Podder, Rachna Rath, Dillip Kumar Muduly, Rajeeb K. Swain, Sunil K. Raghav, et al.9272S), p-AKT (S473) (CST, Cat No# 4058S), GSK-3β (CST, Cat No# 9315s), p-GSK-3β (S9) (CST, Cat No# 9323S), β-catenin (CST, Cat No# 9562). Co-immunoprecipitation SCC9 CisR cell line was lysed in 1% RIPA (CST,

(9) Andrographolide inhibits the upregulation of SLC19A3 to block the adipogenic differentiation of adipose-derived stem cellsiScienceJanuary 16, 2026Yili Yang, Xuan Wang, Guangfeng Zhou, Xiaodan Hou, Yang He, Yuan Feng, Yuxue Jiang, Yuexi Gu, Jun Yemonoclonal anti-SLC19A3,Abcam,Cat# ab237630 Phospho-GSK3β (Ser9) (5B3),Cell Signaling Technology,Cat#9323S; RRID: AB_2115201 Rabbit polyclonal antibody to GSK3 beta,Affinity Biosciences,Cat#AF5016; RRID: AB_2834935 Phospho-β-Catenin (Ser33/37/Thr41) Antibody

(10) GLP-1 agonists reduce right ventricular mitochondrial dysfunction by inhibiting the GSK3β-Drp1 signalling in pulmonary hypertensionEuropean Journal of Medical ResearchJanuary 10, 2026Lin Zhao, Yanghong Liu, Ziwei Ou, Xiexiong Zhao, Wen Zhang, Tangzhiming Li, Zhe Sheng, Ye Chen, Hui Luoovernight at 4 °C with primary antibodies against: Phospho-GSK3β (Ser9) (Cell Signaling Technology, #9323); Phospho-Drp1 (Ser616) (Cell Signaling Technology, #4494); Phospho-Drp1 (Ser637) (Cell Signaling Technology,

(11) LEM4 interacts with β-catenin and promotes β-catenin-dependent transcription activity to confers ribociclib resistance in breast cancer cellsSci RepDecember 24, 2025Qi Tang, Xintong Zhao, Hui Li, Jiaqian Liao, Bingyu Ouyang, Chunxiao Ma, Dedian Chen(1:1000, Cell Signaling Technology, 9315 S), phospho-GSK3β (Ser9) (1:800, Cell Signaling Technology, 9323 S), Histone H3 (1:1000, Cell Signaling Technology, 9715 S), GAPDH (1:2000, Cell Signaling Technology,

(12) Inhibition of GSK3 and TSC2 Mediates the Oncogenic Activity of AKT in Hepatocellular CarcinomaCancer ResearchDecember 15, 2025Guofei Cui, Yi Zhou, Weiting Liao, Eleonora Cossu, Matthias Evert, Shu Zhang, Jingxiao Wang, Shanshan Deng, Andrew Yonemura, Lucie David, et al.1:100; # 9316; RRID:AB_659836; Cell Signaling Technology), and anti-p-GSK3β (Ser9; dilution 1:100; # 9323; RRID:AB_2115201; Cell Signaling Technology) primary antibodies overnight at 4 °C. Subsequently, slides

(13) Resistance exercise training and blueberry extract protect against cardiac and skeletal muscle remodeling and metabolism disruptions in experimental pulmonary arterial hypertensionClinical HypertensionDecember 1, 2025Leôncio Lopes Soares, Luciano Bernardes Leite, Bruno Rocha Avila Pelozin, Sebastião Felipe Ferreira Costa, Thainá Iasbik-Lima, Patrick Turck, Alex Sander da Rosa Araujo, Flavio Gilberto Herter, Tiago Fernandes, Mariana Machado-Neves, et al.(Thr37/46, #9459), eIF4E (#9742), phospho-eIF4E (Ser209, #9741), GSK3β (#9315), phospho-GSK3β (Ser9, #9323), phospho-Bad (Ser136, #9295), Bcl-xL (#2764) and α-Tubulin (#2144). All these antibodies were purchased

(14) Stimulation at the frontal cortex influences the exercise activity and skeletal muscle status in senescence-accelerating micenpj AgingNovember 25, 2025Nobuyuki Nagaoka, Akiko Eguchi, Ning Ma, Jun Kawanokuchi, Teruhisa Yamamoto, Mina Tempaku, Kiora Izuoka, Jun Okabe, Motoh Iwasa, Hayato Nakagawa, et al.(#12456, Cell Signaling Technology, 1:1000), anti-phosphorylated GSK3β at Serine 9 (p-GSK3β (Ser9), #9323 Cell Signaling Technology, 1:1000), anti-mTOR (#2983, Cell Signaling Technology, 1:1000) anti-phosphorylated

(15) IFITM3-MET interaction drives osimertinib resistance through AKT pathway activation in EGFR-mutant non-small cell lung cancerMol CancerOctober 28, 2025Ritsu Ibusuki, Eiji Iwama, Atsushi Shimauchi, Hiromu Kawano, Shun Mizusaki, Satoshi Nakamura, Yui Miyazaki, Yu Inutsuka, Mikiko Hashisako, Taishi Harada, et al.(#9272), to the DYKDDDDK Flag tag (#14793), to phospho-MET (#3077), to MET (#8198), to phospho-GSK3β (#9323), to GSK3β (#9315), to phospho-mTOR (#5536), to mTOR (#2983), or to β-actin (#4970), all of which were

(16) Exosomal transfer of miR-375-3p from pancreatic β cells to hepatocytes impairs hepatic glycogenesis via Rbpj repressionWorld J DiabetesOctober 15, 2025Fang-Zhi Xu, Lin Dou, Xi Wu, Chen-Xi Xia, Dong-Ni Yu, Yong Man, Tao Shen, Xiu-Qing Huangantibodies against phospho-AKT (Ser473, 9271, 1:1000), AKT (11E7, 4685, 1:1000), phospho-GSK-3β (Ser9, 9323, 1:1000) and GSK-3β (D5C5Z, 12456, 1:1000) were purchased from Cell Signaling Technology. Primary antibodies

(17) MLN4924 inhibits macrophage foam cell formation by enhancing autophagy and regulating metabolic phenotypeLipids in health and diseaseOctober 2, 2025Xing L, Bu Y, Shi J, Liu J, Hu F, Zhang M, Fan J.antibodies were purchased from Cell Signaling Technology (9212, 4631, 39749S, 43566S, 2965S, 4060, 9272, 9323, 9332, Danver, USA). Dylight 488 Conjugated AffiniPure goat anti-rabbit IgG was purchased from Boster

(18) Therapeutic assessment of a novel mitochondrial complex I inhibitor in in vitro and in vivo models of Alzheimer's diseaseeBioMedicineOctober 1, 2025Sergey Trushin, Thi Kim Oanh Nguyen, Andrea Stojakovic, Mark Ostroot, J. Trey Deason, Su-Youne Chang, Liang Zhang, Slobodan I. Macura, Toshihiko Nambara, Wenyan Lu, et al.Cell Signaling Technology, cat. #11818, RRID: AB_2687505), p-GSK3β (Ser 9) (1:1000, Cell Signaling Technology, cat. # 9323, RRID: AB_2115201), GSK3β (1:1000, Cell Signaling Technology, cat. # 9832, RRID: AB_10839406), Sirt

(19) Cytoplasmic TRIM24 promotes colorectal cancer cell proliferation by activating Wnt/β-catenin signalingNature CommunicationsSeptember 29, 2025Ya Wang, Yuanbing Yao, Zehong Liu, Shichao Long, Feng Yi, Qing Fang, Dongbo Wu, Qing Zhu, Hongyan Zai, Shuai Xiao, et al.Santa Cruz Biotechnology, USA), p-AKT (Ser473) (R22961, ZEN BIO), AKT (R23412, ZEN BIO), p-GSK3β (Ser9) (9323, Cell Signaling Technology, USA), GSK3β (22104-1-AP, Proteintech), β-catenin (sc-7963, Santa Cruz Biotechnology),

(20) CDK4/6 and BET inhibitors synergistically suppress pancreatic tumor growth and epithelial-to-mesenchymal transition by regulating the GSK3β-mediated Wnt/β-catenin pathwayCancer Drug ResistanceSeptember 26, 2025Jiangning Gu, Zihao Dai, Tianci Shen, Xiang Chen, Zhuo Yang, Shibo Sun, Dan Chen, Haifeng Luo, Xiuli Wang, Jianqiang Xu,Cell Signaling Technology,#3195 anti-Active β-Catenin,Cell Signaling Technology,#8814 anti-Phospho-GSK-3β (Ser9),Cell Signaling Technology,#9323 anti-GSK-3β (D5C5Z),Cell Signaling Technology,#12456 anti-Phospho-Smad3 (Ser423/425),Cell Signaling Technology

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Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb

Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb from Cell Signaling Technology

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