Fig 2: XIAP controls levels of C-RAF to influence cell migration. (A, B) Whole cell lysates were prepared from CRISPR/Cas9 monoclonal U2OS cells and immunoblotted using the indicated antibodies. (C) XIAP KO U2OS cells were transfected with XIAP or empty vector. Whole cell lysates were prepared and immunoblotted using the indicated antibodies. (D) The area of the wound of XIAP null cells (KO 2) treated with the C-RAF inhibitor, BAY 43–9006, was imaged and measured at 0, 8, and 24 h using ImageJ software. The data was normalised with the percentage wound area equalling 100% at 0 h. Each measurement represents data from an individual scratch (6 technical repeats of 3 independent experiments). The % wound area was plotted against time and the area under the curve (% wound area × time(hours)) was calculated and analysed by unpaired students t-test.