Stat3 (D3Z2G) Rabbit mAb 12640S from Cell Signaling Technology

Product Specs
ItemStat3 (D3Z2G) Rabbit mAb
CompanyCell Signaling Technology
Price $347.00 Inquire
Catalog Number12640S
Quantity100 µl
ApplicationsW, IP, IF-IC, FC-FP, ChIP, ChIP-seq
ReactivityH, M, R, Mk
TargetSTAT3
HostRabbit
NCBI Full Gene Namesignal transducer and activator of transcription 3
NCBI Gene AliasesADMIO, ADMIO1, APRF, HIES
ClonalityMonoclonal
Research AreaImmunology and Inflammation
CloneD3Z2G
Molecular Weight79, 86

Questions and Answers

Can this antibody be used to study the role of STAT3 in cancer models?

Yes, the Stat3 (D3Z2G) Rabbit mAb has been utilized in various studies to investigate the role of STAT3 in cancer. For instance, it was employed in research demonstrating that the Farnesoid X receptor promotes non-small cell lung cancer metastasis through the Jak2/STAT3 signaling pathway, highlighting its relevance in cancer biology ().

Is this antibody effective for detecting phosphorylated STAT3 in Western blotting?

The Stat3 (D3Z2G) Rabbit mAb has been shown to be effective in Western blotting applications, as evidenced by its use in studies where it was employed alongside other antibodies to detect both total and phosphorylated forms of STAT3, confirming its utility in analyzing STAT3 activation ().

What species can this antibody be used with for immunofluorescence applications?

The Stat3 (D3Z2G) Rabbit mAb is reactive with human, mouse, rat, and non-human primate samples, making it suitable for immunofluorescence applications across these species, as indicated by its documented usage in various studies ().

Are there any specific experimental conditions under which this antibody has been successfully applied?

The antibody has been successfully applied in various experimental contexts, including immunoprecipitation and chromatin immunoprecipitation assays, as seen in studies investigating the transcriptional regulation of genes involved in cancer and inflammation, demonstrating its versatility in different experimental setups ().

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Website: Technical Support

(1) Biobank of genetically defined murine prostate cancer tumoroids uncovers oncogenic pathways and drug vulnerabilities driven by PTEN-lossCell Reports MethodsApril 20, 2026Jessica Kalla, Thomas Dillinger, Zlata Pavlovicova, Reema Jacob, Emine Atas, Katarina Mišura, Anil Baskan, Kristina Draganić, Andreas Tiefenbacher, Tanja Limberger, et al.,Cell Signaling Technology,Cat#9559; RRID:AB_390810 Rabbit monoclonal anti-STAT3 (D3Z2G),Cell Signaling Technology,Cat#12640; RRID:AB_2629499 Mouse monoclonal anti-TP53 (1C12),Cell Signaling Technology,Cat#2524; RRID:AB_331743 Mouse

(2) Structure matters: commensal Phocaeicola vulgatus lipopolysaccharide induces attenuated microglial activation and preserves neuronal integrityFront Cell NeurosciApril 14, 2026Valentina Mazziotti, Luca De Simone Carone, Francesca Olmeo, Fabrizio Chiodo, Alba Silipo, Antonio Molinaro, Flaviana Di LorenzoUnited States): rabbit anti-MAP2 (#8707; 1:1,000), rabbit anti-iNOS (#nb300-605), rabbit anti-STAT3 (#12640, 1:1,000), rabbit anti-p-STAT3 (#32143, 1:1,000), rabbit anti-Nf-kB p65 (#8242, 1:500), rabbit anti-p-Nf-kB

(3) BMX inhibition overcomes small cell lung cancer chemoresistance by stabilizing E2F1 via ERK1/2-Cyclin D1/CDK4/6 axisSignal Transduct Target TherApril 8, 2026Ting Wu, Shuang Qi, Chenliang Shi, Chao Wu, Qingwang Liu, Chen Hu, Jie Hu, Aoli Wang, Jing Liu, Ziping Qi, et al.(#14220), PARP (#9542), ubiquitin (#3936), p44/42 MAPK (#4695), phospho-p44/42 MAPK (#4370), STAT3 (# 12640), phospho-STAT3 (Tyr705) (#9145), Cyclin D1 (#55506), Rb (#9313), phospho-Rb (Ser807/811) (#8516), Akt

(4) Application of 18β-glycyrrhetinic acid Fluorescent probes in cell imagingJ Enzyme Inhib Med ChemMarch 9, 2026Yifan Zhao, Hongxia Sun, Zhiwei Yan, Youde Wang, Shuai Li, Yachun Guo, Guangxin Miao, Tienan Wang, Liying Zhang, Chengjun Songprotein blotting. GAPDH (8884, CST, 1:5000), HDAC8 (#66042, CST, 1:1500), P-STAT3 (#9154, CST, 1:1500), STAT3 (#12640, CST, 1:1000), SOCS3 (#52113, CST, 1:2000), SDS-PAGE reagents (M00657, Genescript, Nanjing, China). Immunofluorescence Two

(5) Peritoneal MSCs-derived exosomes suppress CCL24 synthesis through miR-320d delivery contributing to the improvement of peritoneal dialysis-associated fibrosisScientific ReportsMarch 4, 2026Xinhui Zhao, Minhui Xi, Hualin Qi, Jia Xu, Rui Yuan, Danye Shi, Jingyuan Lumanufacturer’s instruction. Immunoprecipitation reactions were performed by using antibodies against STAT3 (CST, 12640), and rabbit IgG (CST, 2729). Immunoprecipitated DNA samples were then analyzed by qPCR. The primers

(6) The enhanced osteogenic differentiation of human periodontal ligament stem cells and M2 polarization of macrophages may be mediated by EphB4/ephrinB2 signaling pathway: a study of their direct co-cultureStem Cell Research & TherapyMarch 2, 2026Xiaoqian Yang, Yijun Luan, Jiling Qiu, Huaze Ren, Qiuyue Yin, Hongrui Liu, Hui Song, Aimei SongAB_2139822), anti-p-STAT3 (Tyr705) (1:1000, CST, USA, Cat#9145), anti-STAT3 (1:1000, CST, USA, Cat#12640) and anti-GAPDH (1:1,000,000, HUABIO, USA). After washing, membranes were incubated with HRP-conjugated

(7) p16 Ink4a ‐Positive Hepatocytes Drive Liver Fibrosis Through Activation of LIFR Family PathwayAdvanced ScienceMarch 1, 2026Koji Nishikawa, Teh‐Wei Wang, Satoshi Kawakami, Shota Tanimoto, Kiyoshi Yamaguchi, Taketomo Kido, Masamichi Kimura, Tsunekazu Hishima, Yuki T. Okamura, Satotaka Omori, et al.subjected to immunoblotting with the anti‐p16 (abcam, ab211542, 1:1000 dilution), anti‐STAT3 (CST, 12640, 1:1000 dilution), and anti‐p‐STAT3 (CST, 9145, 1:1000 dilution) using the ECL detection system. Anti‐GAPDH

(8) Mitochondrial-derived peptides MOTS-c and humanin attenuate dexamethasone-induced atrophy in human skeletal muscle cellsPhysiol RepFebruary 24, 2026Rabie Elhusseiny, Mohammed Ihsan, Théo Bellefroid, Abdulaziz Farooq, Sébastien Racinais, Louise DeldicqueUSA): Akt (#9272), p‐Akt (Ser473) (#4060), NF‐κB p65 (#8242), p‐NF‐κB p65 (Ser536) (#3033), STAT3 (#12640), p‐STAT3 (Tyr705) (#9131), caspase‐3 (#14220), and eEF2 (#2332) and from Abcam (Cambridge, UK): OXPHOS

(9) Optimization of Indole- and Pyrazole-fused Glycyrrhetinic Acid Derivatives as Potent PTP1B Inhibitors: In Silico, In Vitro, In Vivo, and Metabolomic StudiesACS Bio & Med Chem AuFebruary 18, 2026Mitzi López-Sánchez, Hannya Mendoza-Mota, Ledy De-la-Cruz-Martínez, Félix Matadamas-Martínez, Diana Laura Torres-Chacón, Rosendo Martínez-Arellano, Juan Francisco Palacios-Espinosa, Jaime Pérez-Villanueva, Martin González-Andrade, José Carlos Páez-Franco, et al.from Biorad. Rabbit anti-PTP1B (5311S), IRS (2382S), p-IRS (2381T), Akt (9272), p-Akt (4060S), STAT3 (12640S), p-STAT3 (9145S), and mouse anti-β actin (3700S) primary antibodies were obtained from Cell Signaling,

(10) The JAK1-STAT1 signaling pathway triggers inflammation responses in chronic obstructive sleep apnea rat modelPLOS OneFebruary 17, 2026Lin Yang, Jie Heanti-STAT1 (1:1,000, CST#14994), anti-p-STAT1 (Tyr701; 1:1,000, CST#9167), anti-STAT3 (1:1,000, CST#12640), anti-p-STAT3 (Tyr705; 1:1,000, CST#9145), and β-actin (1:5,000, CST#4970). HRP-conjugated secondary

(11) Flavones and Aminoflavones Increase the Cytotoxicity of NK Cells in Human Non-Small Cell Lung CancerJ Cell Mol MedFebruary 13, 2026Ping-Chih Hsu, Chih-Cheng Chien, Yang-Je Cheng, Chuan-Hsin Chang, Tzenge-Lien Shihflow cytometry analysis were purchased from BioLegend (San Diego, CA, USA). Anti‐STAT3 antibody (1:1000, #12640), anti‐phospho‐Stat3 (Tyr705) antibody (1:1000, #9131), and anti‐glyceraldehyde‐3‐phosphate dehydrogenase

(12) Targeting immune cells in the aged brain with engineered proteins reveals that engineered cytokine IL-10 enhances neurogenesis and improves cognitionImmunityFebruary 10, 2026Paloma Navarro Navarro Negredo, Justin You, Max Hauptschein, Adam B. Schroer, Daniel J. Richard, Gita C. Abhiraman, Andy P. Tsai, Eric D. Sun, Giulia Notarangelo, Julliana Ramirez-Matias, et al.1:500). We also tried antibodies against phospho-STAT1 (pY701, clone 4a, BD), total STAT3 (D3Z2G, CST 12640), phospho-STAT3 (Ser727, CST 9134) and SOCS3 (Abcam Ab16030, Novus biologicals NBP2-27116, CST 52113)

(13) Low-dose 5-hydroxymethylfurfural mitigates radiation -induced intestinal toxicity via HIF2α-driven IL22/STAT3 signaling enhancementJournal of Translational MedicineFebruary 2, 2026Tao Zhang, Jingqin He, Jiang He, Cheng Xu, Yu Huang, Siting Huang, Haibing Jiang, Yingjie Fan, Li Liu, Hongli Li, et al.The primary antibodies used were anti-phospho-Stat3 (Y705) (D3A7) (9145, CST), anti-Stat3 (D3Z2G) (12640, CST), and anti-HIF-1α (D2U3T) (14179, CST) and anti-HIF-2α (E8E5Z) (71565, CST), anti-IL22R1 (SAB1407826,

(14) Dual TYK2/JAK1 Inhibition by Brepocitinib Reprograms Synoviocyte Pathobiology: Mechanistic Insights Into Targeted Therapy for Rheumatoid ArthritisIranian Journal of Pharmaceutical ResearchFebruary 1, 2026Umar Saeed, Zahra Zahid Piracha, Andromeda M. Nauli, Surya M. Naulip-TYK2 #9321, TYK2 #14193, p-JAK1 #3331, JAK1 #3344, p-STAT1 #7649, STAT1 #14994, p-STAT3 #9145, STAT3 #12640, BAX #2772, BCL-2 #4223, cleaved caspase-3 #9664, caspase-3 #9662, GAPDH #5174). GAPDH was used as a

(15) Total flavonoids extracted from Penthorum chinense Pursh inhibits oxidative stress and inflammation in liver cancer cells through the JAK2/STAT3 pathwayTranslational Cancer ResearchJanuary 31, 2026Xiaoyan Li, Xiaoqiang Zhang, Junchao Xue1:1,000, CST), JAK2 (#3230, 125 kDa, 1:1,000, CST), p-STAT3 (#52075, 86 kDa, 1:1,000, CST), STAT3 (#12640, CST, 79 kDa, 1:1,000, CST), cyclin D1 (#55506, 36 kDa, CST), vascular endothelial growth factor A (VEGFA;

(16) PLB1 enhances adhesion and invasion of Trichosporon asahii to lung epithelial cells: insights into the mechanisms of Cav1/JAK/STAT3-induced arginine metabolismRespiratory ResearchJanuary 23, 2026He Zhu, Junhong Ao, Xiaoxian Cheng, Xin Yang, Haitao Li, Rongya Yanganti-CCL12: ab9737, Abcam; anti-JAK2: 1:1000, 3230, CST; anti-p-JAK2: 1:1000, 4406, CST; anti-STAT3: 1:1000, 12640, CST; anti-p-STAT3: 1:2000, 9145, CST; anti-GAPDH: 1:10000, ab181602, Abcam) at 4°C. The following day,

(17) Gut metabolite indole-3-acetic acid aggravates neuropsychiatric lupus via the AHR/STAT3 pathway in microgliaCommunications BiologyJanuary 23, 2026Yi Feng, Lijuan Zheng, Wenli Tang, Pan Wang, Yanxia Lai, Jiayu Qin, Chang Zhou, Xian Zhang, Min Yang, Ligang Jie, et al.hydrocarbon receptor (AHR, 1:10,000, 67785-1-Ig, Proteintech Group Inc, Rosemont, IL), STAT3 (1:1,000, 12640S, Cell Signaling Technology), p-STAT3 (1:1000, 9131S, Cell Signaling Technology), and glyceraldehyde-3-phosphate

(18) GH Alters Lymphatic Vessels in Female Mice and STAT5 Phosphorylation in Human Lymphatic Endothelial CellsEndocrinologyJanuary 19, 2026Christopher Walsh, Emily Scott, Elise Wagner, Jerome Walsh, Shashank Reddy, Arshad Ahmad, Reetobrata Basu, Eva Sevick-Muraca, Rich Brody, Uday Sandbhor, et al.9145S, RRID: AB_2491009), total signal transducer and activator transcription 3 (STAT3; 1:1000, CST Cat#12640S, RRID: AB_2629499), phosphorylated p44/42 mitogen-activated protein kinase (1:1000, CST Cat# 9101S,

(19) Thimerosal Inhibits Tumor Malignant Progression through Direct Action and Enhancing the Efficacy of PD-1-Based ImmunotherapyOncology ResearchJanuary 19, 2026Ping Wang, Yan-Han Chen, Ze-Tao Zhan, Jun-Xiang Zeng, Yu Chen, Yuan Lin, Tao Chen, Wei-Jie Zhou(1:200, 3331S, RRID: AB_2265057, Cell Signaling Technology, Danvers, MA, USA); anti-STAT3 (1:1000, 12640, RRID: AB_2629499, Cell Signaling Technology, Danvers, MA, USA); anti-p-STAT3 (1:1000, 9145S, RRID:

(20) Lactate attenuates hepatic ischemia-reperfusion injury by reducing intrahepatic Inflammation and suppressing hepatocyte-neutrophil interaction via the IL-33/ST2 pathwayCell Communication and SignalingJanuary 15, 2026Peiyang Fang, Zihan Zhang, Jingjing Zhu, Haozhe Xu, Xiaotong Han, Nan Xu, Xiyu Wang, Longyang Zhou, Xiaonan Du, Hua Jin, et al.Cell signaling technology), phospho STAT3 (diluted 1:2000, 9145T, Cell signaling technology), total-STAT3 (diluted 1:1000, 12640T, Cell signaling technology) and β-ACTIN (diluted 1:5000, ab6276, Abcam) were used. The membranes were

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10/17/2022Good STAT3 Antibody YONG MThis antibody was used to check whether IL-6 gene transfer affects STAT3 expression. The results showed antibody detected STAT3 expression and IL-6 did not enhance STAT3 protein levels in obese mice.
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4/21/2021Detection of Total STAT3 in BV2 Microglia Cells Lisha JoshiIn our experimental project we determined downstream signaling pathways that are activated by lysophosphatidic acid (LPA). We investigated the effect of LPA on inflammatory transcription factors in ... Read Review

Stat3 (D3Z2G) Rabbit mAb

Stat3 (D3Z2G) Rabbit mAb from Cell Signaling Technology

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Stat3 (D3Z2G) Rabbit mAb from Cell Signaling Technology

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