TNF-R1 Antibody

Immunohistochemistry analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: dermis.

Western blot analysis of Mouse Liver cell lysates showing detection of ~55 kDa TNF-R1 protein using Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55 kDa.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-TNF-R1 Polyclonal Antibody (SPC-170) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Punctate nuclear staining, dotty staining in cytoplasm.