P-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free NB100-65392 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemP-Selectin/CD62P Antibody (Psel.KO.2.7) - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $419.00Supplier Page View Company Product Page
Catalog NumberNB100-65392
NCBI Gene AliasesPADGEM, CD62, GMP140, PSEL, CD62P, GRMP, LECAM3
ImmunogenP-selectin transfected 300.19 cells
Concentration1.0 mg/ml
Conjugate/TagUnconjugated
ClonePsel.KO.2.7
ClonalityMonoclonal
HostMouse
FormatPBS
TargetP-Selectin/CD62P
IsotypeIgG1
ApplicationsFlow Cytometry,Immunohistochemistry,Immunoprecipitation,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen
Quantity0.1 mg (also 0.025 mg)
ReactivityEquine,Human,Mouse
NCBI Full Gene Nameselectin P
PurityProtein A purified
Antibody TypePrimary Antibodies
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: NB100-65392 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.

Sizes Available: 0.1 mg (also 0.025 mg)

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Questions and Answers

Can this antibody be used to study P-selectin expression in activated platelets?

Yes, the P-Selectin/CD62P Antibody (Psel.KO.2.7) has been utilized in immunohistochemical staining to assess P-selectin expression in activated platelets. In a study, platelets were stimulated with TRAP-6 and subsequently incubated with the antibody at a dilution of 1:500, demonstrating its effectiveness in this application ().

Is this antibody suitable for use in inflammation studies?

The P-Selectin/CD62P Antibody has been shown to be effective in studies involving inflammation. For instance, in a study where bovine aortic endothelial cells were treated with lipopolysaccharide to induce inflammation, the antibody was used for immunohistochemical staining to evaluate P-selectin expression, indicating its suitability for such applications ().

What species can this antibody be used with in experiments?

This antibody is reactive with multiple species, including human, mouse, and horse, making it versatile for various experimental setups involving these organisms.

How is this antibody typically stored for long-term use?

For long-term storage, the P-Selectin/CD62P Antibody should be aliquoted and stored at -20°C, while avoiding freeze-thaw cycles to maintain its integrity and functionality.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Bio-inspired programmable assembly of shape-memory and blood-reinforced cryogel for hemostasis and functional liver regenerationMater Today BioApril 22, 2026Chen Han, Chen-Yu Zou, Ji-Ye Zhang, Yan-Lin Jiang, Ming Xiong, Rui Wang, Xiu-Zhen Zhang, Ming-Yang Li, Long-Mei Zhao, Ya-Xing Li, et al.on the cryogels were labeled with antibodies against CD42b (ab227669, 1: 150, Abcam, UK) and CD62p (NB100-65392, 1: 150, NOVUS, US). The detailed steps for immunofluorescence are provided in the histological analysis

(2) Interventional real-time molecular MRI for targeting early myocardial injury in a pig modelnpj ImagingFebruary 3, 2025Timo Heidt, Simon Reiss, Julien Thielmann, Christian Weber, Alexander Maier, Thomas Lottner, Heidi R. Cristina-Schmitz, Timon Bühler, Diana Chiang, Claus Jülicher, et al.Waltham, MA, USA) at RT for 1 h. Samples were incubated with a primary mouse antibody anti-P-selectin (NB100-65392, Novus Biologicals, C), USA) at 4 oC overnight. After washing, samples were incubated with Alexa Fluor

(3) Interventional real-time molecular MRI for targeting early myocardial injury in a pig modelnpj ImagingFebruary 3, 2025Timo Heidt, Simon Reiss, Julien Thielmann, Christian Weber, Alexander Maier, Thomas Lottner, Heidi R. Cristina-Schmitz, Timon Bühler, Diana Chiang, Claus Jülicher, et al.Waltham, MA, USA) at RT for 1 h. Samples were incubated with a primary mouse antibody anti-P-selectin (NB100-65392, Novus Biologicals, C), USA) at 4 oC overnight. After washing, samples were incubated with Alexa Fluor

(4) Hypothermia-induced activation of the splenic platelet pool as a risk factor for thrombotic disease in a mouse modelJournal of thrombosis and haemostasis : JTHJanuary 1, 0001"Horioka K, Tanaka H, Isozaki S, Okuda K, Asari M, Shiono H, Ogawa K, Shimizu K."

(5) Treatment of hepatocellular carcinoma with autologous platelets encapsulating sorafenib or lenvatinib: A novel therapy exploiting tumor-platelet interactionsInternational journal of cancerJanuary 1, 0001"Tanaka H, Horioka K, Hasebe T, Sawada K, Nakajima S, Konishi H, Isozaki S, Goto M, Fujii Y, Kamikokura Y, et al."

(6) Rapid accumulation of polymorphonuclear neutrophils in the Corpus luteum during prostaglandin F(2α)-induced luteolysis in the cowPloS oneJanuary 1, 0001"Shirasuna K, Jiemtaweeboon S, Raddatz S, Nitta A, Schuberth HJ, Bollwein H, Shimizu T, Miyamoto A."

(7) Effects of clopidogrel on horses with experimentally induced endotoxemiaAmerican journal of veterinary researchJanuary 1, 0001"Watts AE, Ness SL, Divers TJ, Fubini SL, Frye AH, Stokol T, Cummings KJ, Brooks MB."

(8) Effects of clopidogrel on the platelet activation response in horsesAmerican journal of veterinary researchJanuary 1, 0001"Brooks MB, Divers TJ, Watts AE, Ness SL, Frye AH, Stokol T, Fubini SL."

(9) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist deviceArtificial organsJanuary 1, 0001"Johnson CA Jr, Wearden PD, Kocyildirim E, Maul TM, Woolley JR, Ye SH, Strickler EM, Borovetz HS, Wagner WR."

(10) Equid herpesvirus type 1 activates plateletsPloS oneJanuary 1, 0001"Stokol T, Yeo WM, Burnett D, DeAngelis N, Huang T, Osterrieder N, Catalfamo J."

(11) "Unfractionated and Low-Molecular-Weight Heparin and the Phosphodiesterase Inhibitors, IBMX and Cilostazol, Block Ex Vivo Equid Herpesvirus Type-1-Induced Platelet Activation"Frontiers in veterinary scienceJanuary 1, 0001"Stokol T, Serpa PBS, Zahid MN, Brooks MB."

(12) Subcutaneous Administration of Low-Molecular-Weight Heparin to Horses Inhibits Ex Vivo Equine Herpesvirus Type 1-Induced Platelet ActivationFrontiers in veterinary scienceJanuary 1, 0001"Stokol T, Serpa PBS, Brooks MB, Divers T, Ness S."

(13) Pharmacokinetics and Pharmacodynamics of an Oral Formulation of Apixaban in Horses After Oral and Intravenous AdministrationFrontiers in veterinary scienceJanuary 1, 0001"Serpa PBS, Brooks MB, Divers T, Ness S, Birschmann I, Papich MG, Stokol T."

(14) Rewarming from accidental hypothermia enhances whole blood clotting properties in a murine modelThrombosis researchJanuary 1, 0001"Horioka K, Tanaka H, Isozaki S, Konishi H, Addo L, Takauji S, Druid H."

(15) Characterization of platelet and soluble-porcine P-selectin (CD62P)Veterinary immunology and immunopathologyJanuary 1, 0001"Massaguer A, Engel P, Tovar V, March S, Rigol M, Solanes N, Bosch J, Pizcueta P."

(16) Cold atmospheric plasma-enabled platelet vesicle incorporated iron oxide nano-propellers for thrombolysisMaterials today. BioJanuary 1, 0001"Jheng PR, Chiang CC, Kang JH, Fan YJ, Wu KC, Chen YT, Liang JW, Bolouki N, Lee JW, Hsieh JH, et al."

(17) P-Selectin mediates targeting of a self-assembling phototherapeutic nanovehicle enclosing dipyridamole for managing thrombosesJournal of nanobiotechnologyJanuary 1, 0001"Liu CH, Jheng PR, Rethi L, Godugu C, Lee CY, Chen YT, Nguyen HT, Chuang EY."(DFC7000T, Leica). Immunohistochemical (IHC) staining was used to assess the expression of P-selectin (NB100-65392, 1:500, 1 h, NOVUS) by cells, and observed by fluorescence microscopy (DFC7000T, Leica). Fluorescence

(18) Investigating the Role of Endothelial Glycogen Synthase Kinase3α/β in Atherogenesis in Low Density Lipoprotein Receptor Knockout MiceInternational journal of molecular sciencesJanuary 1, 0001"Mastrogiacomo L, Werstuck GH."aortic sinus were immunostained with antibodies against P-selectin (Novus Biologicals, Littleton, CO, NB100-65392 1:50 dilution), E-selectin (Novus Biologicals, Littleton, CO, USA, NBP1-45545 1:100 dilution), VCAM-1

(19) Lung injury-induced activated endothelial cell states persist in aging-associated progressive fibrosisNature communicationsJanuary 1, 0001"Raslan AA, Pham TX, Lee J, Kontodimas K, Tilston-Lunel A, Schmottlach J, Hong J, Dinc T, Bujor AM, Caporarello N, et al."clone 33.1, Biolegend, 1:100 dilution), anti-ACKR1 (NB100-2421, Novus, 1:50 dilution), anti-P-Selectin (NB100-65392, Novus, 1:50 dilution), anti-CTHRC1 (PA5-49638, Invitrogen, Waltham, MA, USA, 1:50 dilution), anti-rabbit

(20) The Effects of Hyperglycemia on Early Endothelial Activation and the Initiation of AtherosclerosisThe American journal of pathologyJanuary 1, 0001"Mastrogiacomo L, Ballagh R, Venegas-Pino DE, Kaur H, Shi P, Werstuck GH."

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