Aminopeptidase N/CD13 Antibody (ER-BMDM1) - BSA Free NB100-64843 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemAminopeptidase N/CD13 Antibody (ER-BMDM1) - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $429.00Supplier Page View Company Product Page
Catalog NumberNB100-64843
NCBI Gene AliasesP150, PEPN, LAP1, Cd13, APN, AP-M, AP-N, GP150, Apn, hAPN, CD13
ImmunogenAminopeptidase N/CD13 Antibody (ER-BMDM1) made from Balb/c bone marrow derived macrophages
Concentration1.0 mg/ml
IsotypeIgG2a
ReactivityMouse
ClonalityMonoclonal
HostRat
Quantity0.125 mg (also 0.025 mg)
TargetAminopeptidase N/CD13
CloneER-BMDM1
Conjugate/TagUnconjugated
ApplicationsIF/IHC,Flow Cytometry,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen
FormatPBS
NCBI Full Gene Namealanyl (membrane) aminopeptidase, alanyl aminopeptidase, membrane
PurityProtein G purified
Research AreaAngiogenesis, Cellular Markers, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Myeloid derived Suppressor Cell, Stem Cell Markers
Antibody TypePrimary Antibodies
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: NB100-64843 recognizes the murine homologue of human CD13.

Sizes Available: 0.125 mg (also 0.025 mg)

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Questions and Answers

Can this antibody be used for immunofluorescence staining in mouse liver tissue?

Yes, the Aminopeptidase N/CD13 Antibody (ER-BMDM1) has been successfully used for immunofluorescence staining in mouse liver tissue, as demonstrated in studies where it was applied at a dilution of 1:500 for detecting CD13 in fixed liver samples ().

Is this antibody effective for flow cytometry applications?

The Aminopeptidase N/CD13 Antibody has been utilized in flow cytometry, as indicated in protocols for isolating primary murine brain endothelial cells, where it was included in a cocktail of antibodies for identifying specific cell populations ().

What is the specificity of this antibody in terms of species reactivity?

This antibody specifically recognizes the murine homologue of human CD13, making it suitable for use in mouse models ().

Has this antibody been cited in any relevant scientific literature?

Yes, the Aminopeptidase N/CD13 Antibody has been cited in at least seven scientific articles, indicating its relevance and application in various research contexts (, , , ).

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Liquid-crystal organization of liver tissueeLifeJune 17, 2019Hernán Morales-Navarrete, Hidenori Nonaka, André Scholich, Fabián Segovia-Miranda, Walter de Back, Kirstin Meyer, Roman L Bogorad, Victor Koteliansky, Lutz Brusch, Yannis Kalaidzidis, et al.polyclonal) Millipore AB2033/RRID:AB_2105702 (1:1000) Antibody anti-CD13 (rat monoclonal) Novus NB100 64843/RRID:AB_959651 (1:500) Antibody anti-integrin ß1 (rat monoclonal) Millipore MAB1997/RRID:AB_2128202 (1:1000) Antibody Donkey

(2) Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1Nature medicineJune 1, 2019Yousef H, Czupalla CJ, Lee D, Chen MB, Burke AN, Zera KA, Zandstra J, Berber E, Lehallier B, Mathur V, et al.eBioscience, 45–5921-80, clone TER119) Anti- Mouse CD13 Antibody (CD13-APC) (1:50, NOVUS Biologicals, NB100–64843, clone ER-BMDM1) Anti-Mouse ACSA-2 PE (1:100, Miltenyi Biotec Inc., 130–102-365, clone IH3–18A3) Anti-Mouse

(3) Collagenase-based Single Cell Isolation of Primary Murine Brain Endothelial Cells Using Flow CytometryBio-protocolJanuary 1, 0001"Czupalla CJ, Yousef H, Wyss-Coray T, Butcher EC."catalog number: 53024) 40. Aminopeptidase N/CD13 Antibody (ER-BMDM1) (NOVUS Biologicals, catalog number: NB100-64843) 41. ACSA-2 (Miltenyi, catalog number: 130-097-674) Copyright © 2018 The Authors; exclusive licensee

(4) Quantification of nematic cell polarity in three-dimensional tissuesPLoS computational biologyJanuary 1, 0001"Scholich A, Syga S, Morales-Navarrete H, Segovia-Miranda F, Nonaka H, Meyer K, de Back W, Brusch L, Kalaidzidis Y, Zerial M, et al."and liver tissue, respectively, were identified by staining fixed cells with anti-CD13 (Novus, cat NB100-64843, rat, 1/500) as reported previously [14, 15]; example raw images are shown in Fig S1A in S1B Appendix.

(5) Hepatocyte differentiation requires anisotropic expansion of bile canaliculi"Development (Cambridge, England)"January 1, 0001"Bebelman MP, Belicova L, Gralinska E, Jumel T, Lahree A, Sommer S, Shevchenko A, Zatsepin T, Kalaidzidis Y, Vingron M, et al."primary antibodies diluted in blocking buffer at 4°C overnight. Primary antibodies used were: anti-CD13 (NB100-64843; rat; Novus Biologicals; 1:500), anti-NMIIA (GTX113236; rabbit; GeneTex; 1:100), anti-Desmin (AF3844;

(6) Anisotropic expansion of hepatocyte lumina enforced by apical bulkheadsThe Journal of cell biologyJanuary 1, 0001"Belicova L, Repnik U, Delpierre J, Gralinska E, Seifert S, Valenzuela JI, Morales-Navarrete HA, Franke C, Räägel H, Shcherbinina E, et al."Primary antibodies were diluted in the blocking solution, rat monoclonal anti-CD13 (Novus; cat. no. NB100-64843; RRID:AB_959651; 1:500), rabbit polyclonal anti-ZO-1 (Thermo Fisher Scientific; cat. no. 40–2200; RRID:AB_2533456;

(7) Apical bulkheads accumulate as adaptive response to impaired bile flow in liver diseaseEMBO reportsJanuary 1, 0001"Mayer C, Nehring S, Kücken M, Repnik U, Seifert S, Sljukic A, Delpierre J, Morales-Navarrete H, Hinz S, Brosch M, et al."antibodies Rabbit anti BSEP,Sigma,HPA019035 Mouse anti CD13,Santa Cruz,sc‐136484 Rat anti CD13,Novus,NB100‐64843 Goat anti DPPIV,R&D,AF1180 Rabbit anti Fibronectin,Lifespan Biosciences,LS‐B2318 Rabbit anti GS,Sigma,G2781 Rabbit

(8) Hepatocyte apical bulkheads provide a mechanical means to oppose bile pressureThe Journal of cell biologyJanuary 1, 0001"Bebelman MP, Bovyn MJ, Mayer CM, Delpierre J, Naumann R, Martins NP, Honigmann A, Kalaidzidis Y, Haas PA, Zerial M."permeabilized with 0.5% Triton X-100 in PBS for 1 h at RT. Primary antibodies against CD13 (cat. no. NB100-64843; Rat; Novus) and ZO-1 (cat. no. 40-2200; Rabbit; Invitrogen) were diluted in Tx buffer (0.2% gelatin,

(9) Liquid-crystal organization of liver tissueeLifeJanuary 1, 0001"Morales-Navarrete H, Nonaka H, Scholich A, Segovia-Miranda F, de Back W, Meyer K, Bogorad RL, Koteliansky V, Brusch L, Kalaidzidis Y, et al."polyclonal) Millipore AB2033/RRID:AB_2105702 (1:1000) Antibody anti-CD13 (rat monoclonal) Novus NB100 64843/RRID:AB_959651 (1:500) Antibody anti-integrin ß1 (rat monoclonal) Millipore MAB1997/RRID:AB_2128202 (1:1000) Antibody Donkey

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