5'-Nucleotidase/CD73 Antibody AF4488 from R&D Systems, a Bio-Techne Brand

Product Specs
Item5'-Nucleotidase/CD73 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $539.00Supplier Page View Company Product Page
Catalog NumberAF4488
NCBI Gene AliaseseN, CALJA, CD73, NTE, NT5, E5NT, 5'-NT, eNT, Nt5, 2210401F01Rik, NT
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunocytochemistry,CyTOF-ready
HostSheep
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityMouse,Porcine,Rat
IsotypeIgG
Target5'-Nucleotidase/CD73
Quantity100 ug (also 25 ug)
NCBI Full Gene Name5' nucleotidase, ecto, 5'-nucleotidase ecto
ClonalityPolyclonal
ImmunogenChinese hamster ovary cell line CHO-derived recombinant mouse 5'-Nucleotidase/CD73, Trp29-Lys549, Accession # Q61503
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse 5'-Nucleotidase/CD73 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) The impact of hypoxia preconditioning on mesenchymal stem cells performance in hypertensive kidney diseaseStem Cell Research & TherapyJune 9, 2024Gurparneet Kaur Sohi, Naba Farooqui, Arjunmohan Mohan, Kamalnath Sankaran Rajagopalan, Li Xing, Xiang Y. Zhu, Kyra Jordan, James D. Krier, Ishran M. Saadiq, Hui Tang, et al.confirm the expression of MSC-specific surface markers CD73 (R&D Systems®, Minneapolis, MN, USA, Cat. # AF4488), CD90 (Abcam, San Francisco, CA, USA Cat. # ab124527), and CD105 (Abcam, Cat. # ab53321). Same way,

(2) Alginate-Encapsulated Mesenchymal Stromal Cells Improve Hind Limb Ischemia in a Translational Swine ModelJournal of the American Heart AssociationMay 7, 2024Deppen JN, Ginn SC, Tang EO, Wang L, Brockman ML, Levit RD.(Aurora; Cytek Biosciences) with fluorescently labeled antibodies (Alexa Fluor 647 CD73 [R&D Systems AF4488 with Abcam ab150179], APC/Fire 750 CD90 [BioLegend 328137], PE CD105 [Abcam ab53321], fluorescein isothiocyanate

(3) Role of Ecto-5’-nucleotidase in Bladder FunctionFASEB JJanuary 31, 2024Sagar Barge, Ali Wu, Lanlan Zhang, Simon C. Robson, Aria Olumi, Seth L. Alper, Mark L. Zeidel, Weiqun YuRRID: AB_10572702), goat anti-Alpl (1:1000, #AF2910; RRID: AB_664062), and sheep anti-Nt5e (1:1000, #AF4488; RRID: AB_951934) antibodies were from R&D system (Minneapolis, MN, USA). Bladder samples of male wild

(4) Role of ecto‐5′‐nucleotidase in bladder functionThe FASEB JournalJanuary 31, 2024Sagar Barge, Ali Wu, Lanlan Zhang, Simon C. Robson, Aria Olumi, Seth L. Alper, Mark L. Zeidel, Weiqun YuRRID: AB_10572702), goat anti-Alpl (1:1000, #AF2910; RRID: AB_664062), and sheep anti-Nt5e (1:1000, #AF4488; RRID: AB_951934) antibodies were from R&D system (Minneapolis, MN, USA). Bladder samples of male wild

(5) Characterization of the structure, vascularity, and stem/progenitor cell populations in porcine Achilles tendon (PAT)Cell and tissue researchMay 1, 2021Zhang J, Li F, Williamson KM, Tan S, Scott D, Onishi K, Hogan MV, Wang JH.anti-elastin (1:500, Abcam, Cat. ab21610, Cambridge, MA), sheep anti-CD73 (1:350, R & D Systems, Cat. AF4488, Minneapolis, MN), mouse anti-osteocalcin (1:400, ThermoFisher Scientific, Cat. MA1–20786, Pittsburgh,

(6) Hypoxic preconditioning induces epigenetic changes and modifies swine mesenchymal stem cell angiogenesis and senescence in experimental atherosclerotic renal artery stenosisStem cell research & therapyApril 14, 2021Isik B, Thaler R, Goksu BB, Conley SM, Al-Khafaji H, Mohan A, Afarideh M, Abumoawad AM, Zhu XY, Krier JD, et al.confirm expression of MSC-specific surface markers CD73 (R&D Systems®, Minneapolis, MN, USA, Cat.# AF4488), CD90 (Abcam, San Francisco, CA, USA Cat.# ab124527), and CD105 (Abcam, Cat.# ab53321). Conversely,

(7) Kidney inflammaging is promoted by CCR2(+) macrophages and tissue-derived micro-environmental factorsCellular and molecular life sciences : CMLSApril 1, 2021Lefèvre L, Iacovoni JS, Martini H, Bellière J, Maggiorani D, Dutaur M, Marsal DJ, Decaunes P, Pizzinat N, Mialet-Perez J, et al.anti CCR2 (Santa-Cruz, SC6228) or rat anti Sca-1 (Biolegend, clone E13-161.7) and sheep anti CD73 (AF4488, Novus Biological) diluted in blocking buffer overnight at 4 °C. After washing twice for 10 min in washing

(8) Perivascular CD73(+) cells attenuate inflammation and interstitial fibrosis in the kidney microenvironmentAmerican journal of physiology. Renal physiologySeptember 1, 2019Perry HM, Görldt N, Sung SJ, Huang L, Rudnicka KP, Encarnacion IM, Bajwa A, Tanaka S, Poudel N, Yao J, et al.in 0.1 M phosphate buffer for 15–30 min. Cells were labeled with sheep anti-porcine CD73 [2 ␮g/ml, AF4488, R&D Systems, direct tagged using the Alexa Fluor 555 or Alexa Fluor 647 Antibody/Protein Labeling Kit (ThermoFisher)

(9) Dysregulation of ectonucleotidase-mediated extracellular adenosine during postmenopausal bone lossScience advancesAugust 1, 2019Shih YV, Liu M, Kwon SK, Iida M, Gong Y, Sangaj N, Varghese S.were then incubated with primary antibody against CD39 (5 μg/ml; AF4398, R&D Systems), CD73 (5 μg/ml; AF4488, R&D Systems), and A2BR (1:200; MBS8207549, MyBioSource, San Diego, CA) in diluent solution (1%, w/v)

(10) Molecular- and microarray-based analysis of diversity among resting and osteogenically induced porcine mesenchymal stromal cells of several tissue originJournal of tissue engineering and regenerative medicineJanuary 1, 2018Bayraktar S, Jungbluth P, Deenen R, Grassmann J, Schneppendahl J, Eschbach D, Scholz A, Windolf J, Suschek CV, Grotheer V.

(11) Expression of Prostatic Acid Phosphatase in Rat Circumvallate PapillaePloS oneJanuary 1, 2016Nishida K, Kubota T, Matsumoto S, Kato J, Watanabe Y, Yamamoto A, Furui M, Ohishi A, Nagasawa K.Aves Labs)" NT5E(CD73),Sheep anti-NT5E Ab,Donkey anti-sheep IgG conjugated with Alexa Fluor 594 NT5E(CD73),"(1:100; #AF4488, R&D Systems)","(1:1000; #A11016, Life Technologies™)" NTPDase2,Sheep anti-NTPDase2 Ab,Donkey anti-sheep

(12) FACS array profiling identifies Ecto-5' nucleotidase as a striatopallidal neuron-specific gene involved in striatal-dependent learningThe Journal of neuroscience : the official journal of the Society for NeuroscienceMay 15, 2013Ena SL, De Backer JF, Schiffmann SN, de Kerchove d'Exaerde A.immunofluorescence, the anti-GFP antibody was coincubated overnight at 4°C with sheep anti-NT5e (1:200; AF4488; R&D Systems) (Sowa et al., 2010) or mouse monoclonal anti-NeuN (1:100; MAB377; Millipore). Mouse monoclonal

(13) Adenosine enhances sweet taste through A2B receptors in the taste budThe Journal of neuroscience : the official journal of the Society for NeuroscienceJanuary 4, 2012Dando R, Dvoryanchikov G, Pereira E, Chaudhari N, Roper SD.(Dvoryanchikov et al., 2009). Immunofluorescence for NT5E used 1:100 sheep anti-5⬘-nucleotidase antibody (AF4488, R&D Systems) followed by 1:1000 donkey anti-sheep IgG-Alexa Fluor 568 (A-21099, Invitrogen). Reagents

(14) Recombinant ecto-5'-nucleotidase (CD73) has long lasting antinociceptive effects that are dependent on adenosine A1 receptor activationMolecular painApril 14, 2010Sowa NA, Voss MK, Zylka MJ.(Pierce/ Thermo Scientific, Cat. # 24590) and western blotting with anti-NT5E antibody (R&D Systems, AF4488). Amersham full-range rainbow molecular weight markers (GE Healthcare) were used for SDS-PAGE and western blots.

(15) Ecto-5'-nucleotidase (CD73) inhibits nociception by hydrolyzing AMP to adenosine in nociceptive circuitsThe Journal of neuroscience : the official journal of the Society for NeuroscienceFebruary 10, 2010Sowa NA, Taylor-Blake B, Zylka MJ.Mrgprd⌬EGFPf knock-in mice with antibodies to GFP (Zylka et al., 2005). Antibodies used included anti-NT5E (AF4488, R&D Systems; 1:50 for DRG and skin and 1:100 for spinal cord), rabbit anti-CGRP (T-4032; Peninsula;

(16) BMP-2-Driven Osteogenesis: A Comparative Analysis of Porcine BMSCs and ASCs and the Role of TGF-β and FGF SignalingBiologyJanuary 1, 0001"Taday R, Jungbluth P, Zensen S, Krakau T, Windolf J, Hoffmann MJ, Grotheer V."MN, USA), CD26 (, Cat. No.: NB600-552APC, Novus Biologicals, Minneapolis, MN, USA), CD73 (, Cat. No.: AF4488, R&D Systems, Minneapolis, MN, USA), CD90 (Cat. No.: 559,869, BD Pharmingen, Franklin Lakes, NJ, USA),

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5'-Nucleotidase/CD73 Antibody

5'-Nucleotidase/CD73 Antibody

5'-Nucleotidase/CD73 Antibody

5'-Nucleotidase/CD73 Antibody

5'-Nucleotidase/CD73 Antibody

5'-Nucleotidase/CD73 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (16)

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5'-Nucleotidase/CD73 Antibody from R&D Systems, a Bio-Techne Brand

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