Anti-GFAP Antibody Picoband® PA1239 from BosterBio

Product Specs
ItemAnti-GFAP Antibody Picoband®
CompanyBosterBio
Price $370.00Supplier Page View Company Product Page
Catalog NumberPA1239
Quantity100 µg/vial
ApplicationsIF,IHC,WB
FormatLyophilized, Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
PurityImmunogen affinity purified
ReactivityHuman,Mouse,Rat
ImmunogenA synthetic peptide corresponding to a sequence at the C-terminus of human GFAP, identical to the related rat sequence, and different from the related mouse sequence by two amino acids
ClonalityPolyclonal
Gene NameGFAP
NCBI Gene AliasesALXDRD
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 µg/ml
HostRabbit
Molecular Weight49880 MW, Observed: 49 kDa
Calculated: 49880 MW
Research AreaCell Adhesion Proteins,Cell Type Marker,Cell Type Markers,Cytoskeleton,Cytoskeleton/ECM,Intermediate Filaments,Neural Stem Cells,Neuroscience,Signal Transduction,Stem Cells,Tags & Cell Markers
StorageStore at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles
IsotypeIgG
TargetGlial fibrillary acidic protein
Antibody TypePrimary Antibodies
Conjugate/TagBiotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647, carrier-free

Questions and Answers

Can this antibody be used to study astrocyte activation in mouse models?

Yes, the Anti-GFAP Antibody has been utilized in studies involving mouse models, such as the AdKO mouse model for Cushing's syndrome, where it was used to assess the expression of glial fibrillary acidic protein (GFAP) as a marker for reactive astrocytes. In this context, decreased GFAP expression was observed in specific brain regions, indicating its relevance for studying astrocyte activation.

What applications has this antibody been tested in?

The Anti-GFAP Antibody has been tested in immunofluorescence (IF), immunohistochemistry (IHC), and western blotting (WB) applications. This versatility allows researchers to use it in various experimental setups to detect GFAP in different sample types.

Is this antibody suitable for detecting GFAP in human tissues?

Yes, the Anti-GFAP Antibody is reactive with human tissues, as well as mouse and rat tissues. This makes it suitable for studies involving human samples, particularly in the context of neurobiology and astrocyte research.

What is the recommended dilution for using this antibody in immunohistochemistry?

In the referenced study, the Anti-GFAP Antibody was used at a dilution of 1:200 for immunohistochemistry. This dilution can serve as a guideline, but optimization may be necessary depending on specific experimental conditions.