Fig 1: The KRAI sequence is mapped as the nuclear localization signal (NLS) of Crlz-1(A) The KRAI sequence of four amino acids (aa) from aa 398–401, as indicated by the red dashed circle over the site-directed mutant (sm) constructs at the bottom, turned out to be the NLS of mouse Crlz-1. The various C- and/or N-terminal truncation (CΔ, NΔ, and NCΔ) and sm constructs with their N-terminal ends labeled with GFP are called GFP-CΔCrlz-1, GFP-NΔCrlz-1, GFP-NCΔCrlz-1, and GFP-smCrlz-1 with the remaining or sm amino acids numbered and/or written within their corresponding parentheses. They are schematically drawn in the center, while their corresponding names and cellular locations of expressed proteins are aligned on their left and right sides, respectively. A reference linear view of the full-length (aa 1–469) wild-type (wt) mouse Crlz-1, where the amino acid residues are counted from N to C termini, is drawn at the top and includes two leucine zipper-like domains (LZ1 and LZ2, boxes) and one Sas10 homology domain (Sas10H, oval), as defined by its original cloner. The representative halogen and fluorescent images showing the nuclear localization of GFP-wtCrlz-1 expressed in NIH3T3 cells are given at the top right. (B) The conserved NLS motifs with their surrounding amino acid sequences (right) from 13 species of nearby as well as distant taxonomic lineages are aligned with their corresponding species names (left). As compared to KRAI as the NLS of mouse Crlz-1, the identically or similarly conserved amino acids are colored red or blue, respectively.