Fig 1: Identification of bovine macrophage. Bovine monocytes were derived from peripheral blood and differentiated into macrophages. (A) Forward scatter (FSC) vs. side scatter (SSC) plot of bovine monocyte-derived macrophages (BMDM), (B) flow cytometry analysis of unstained cells, and (C) flow cytometry analysis of cells stained with (SIRPA anti-bovine CD172a) antibody conjugated with RPE-Cy5. The percentage purity of the macrophage population is shown (R2). The cells were stained using TRITC-conjugated anti-CD16 antibody in combination with FITC-conjugated phalloidin for actin staining and DAPI for cell nuclei (D). Experiments were performed in triplicate and repeated three times (n = 9).