IL-6 (D3K2N) Rabbit mAb 12153S from Cell Signaling Technology

Product Specs
ItemIL-6 (D3K2N) Rabbit mAb
CompanyCell Signaling Technology
Price $359.00 Inquire
Catalog Number12153S
Quantity100 µl
ApplicationsW, W-S, IP
ReactivityH
ClonalityMonoclonal
TargetIL6
HostRabbit
NCBI Full Gene Nameinterleukin 6
NCBI Gene AliasesBSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6
Research AreaImmunology and Inflammation
CloneD3K2N
Molecular Weight21-28

Questions and Answers

Can this IL-6 antibody be used to study inflammatory responses in human cell lines?

Yes, the IL-6 (D3K2N) Rabbit mAb has been shown to effectively detect IL-6 levels in various studies involving human cell lines. For instance, it was utilized in Western blotting to assess IL-6 expression in response to inflammatory stimuli, demonstrating its applicability in studying inflammatory responses in human cells.

Is this antibody suitable for use in immunoprecipitation experiments?

The IL-6 (D3K2N) Rabbit mAb is suitable for immunoprecipitation (IP) applications, as indicated by its documented use in scientific literature. This allows researchers to isolate IL-6 and study its interactions with other proteins, which is crucial for understanding its role in various biological processes.

How has this antibody been utilized in research related to cancer or inflammation?

The IL-6 antibody has been cited in multiple studies focusing on inflammation and cancer. For example, it was used to measure IL-6 levels in the context of tumor microenvironments, highlighting its role in promoting tumor growth and inflammation, as seen in research on diffuse intrinsic pontine glioma (DIPG) and other cancer models.

What is the molecular weight of the IL-6 protein detected by this antibody?

The IL-6 protein recognized by the IL-6 (D3K2N) Rabbit mAb has a molecular weight ranging from 21 to 28 kDa. This information is essential for researchers to confirm the specificity of their Western blot results when analyzing IL-6 expression.

Cell Signaling Technology

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Schisandrin B suppresses cholangiocarcinoma by targeting the ROS/p38 MAPK/NF‑κB axisOncology LettersMarch 26, 2026Junhao Yang, Wenying Long, Xiaoxiao Wu, Xiaohui Yang, Qiang Hong, Shuai Wang(Ser32),2859,"1:1,000","Cell Signaling Technology, Inc." IκBα,4814,"1:1,000","Cell Signaling Technology, Inc." IL-6,12153,"1:1,000","Cell Signaling Technology, Inc." IL-8,94407,"1:1,000","Cell Signaling Technology, Inc." TNF-α,6945,"1:1,000","Cell Signaling Technology

(2) Integrative biology shows DPP4 affects inflammatory response to eclampsia and cell model growth via p65/NLRP3/ASC/Caspase-1 pathwayFront GenetMarch 4, 2026Zhimin Bian, Li Hao, Rongjuan Yang, Jianghui Sunprimary antibodies: DPP4 (Abcam, Catalog No. ab133605, UK), IL6 (Cell Signaling Technology, Catalog No. 12153, United States), TNF-α (Cell Signaling Technology, Catalog No. 3707, USA), GAPDH (Cell Signaling Technology,

(3) Osimertinib activates a TGF-β2-dependent secretory program that drives lung adenocarcinoma progressionJournal of Clinical InvestigationFebruary 2, 2026Madhurima Ghosh, Chao Wu, Abhishek Kumar, Monique B. Nilsson, John V. Heymach, Weina Zhao, Jiang Yu, Xin Liu, Na Ding, Shike Wang, et al.(catalog 4879S), GM130 (catalog 12480S), β-actin (catalog 4970S), p65 (catalog 8242T), IL-6 (catalog 12153), p-STAT3 (catalog 9131S), STAT-3 (catalog 9132) from Cell Signaling Technology; primary antibodies

(4) Piezo1 knockdown activates PI3K/AKT and enhances SPP1 to drive M2 macrophage polarization and reduce cardiac inflammationScientific ReportsJanuary 8, 2026Yunhan Zhang, Ying Zhang, Jiaoyan Song, Ziwen Zhao, Wenhao Ju, Hao Zhang, Shuang Li, Hanlu Li, Qiuzhe Guo, Yinbo Ma, et al.(Proteintech, 15939-1-AP, 1:500), IL-1β (Proteintech, 26048-1-AP, 1:1000), IL-6 (Cell Signaling Technology, 12153, 1:1000), IL-10RA (Proteintech, 13356-1-AP, 1:1000), Phospho-Akt (Cell Signaling Technology, 5012S,

(5) Targeting Tris(2,3-dibromopropyl) Isocyanurate-Induced Inflammation in Hippocampal Neurons In Vitro: Mechanistic Insights and Implications for Neurodegenerative Disease PreventionMolecular NeurobiologyJanuary 1, 2026Dominika Szlachcikowska, Oliwia Koszła, Przemysław Sołek, Anna Tabęcka-Łonczyńskas,Rabbit,1:1000,CellSignaling,40435 Primary,Anti-p-eIF2α,Rabbit,1:1000,CellSignaling,3398 Primary,Anti-ATF4,Rabbit,1:1000,CellSignaling,11815 Primary,Anti-Nrf2,Rabbit,1:1000,CellSignaling,12721 Primary,Anti-Keap1,Rabbit,1:1000,CellSignaling,8047 Primary,Anti-HO-1,Rabbit,1:1000,CellSignaling,43966 Primary,Anti-NQO1,Rabbit,1:1000,CellSignaling,62262 Primary,Anti-TNF-α,Rabbit,1:1000,CellSignaling,8184 Primary,Anti-IL-1β,Rabbit,1:1000,CellSignaling,12703 Primary,Anti-IL6,Rabbit,1:1000,CellSignaling,12153 Primary,Anti-GAPDH,Mouse,1:100 000,ABClonal,AC033 ,Anti-β-actin,Rabbit,1:1000,CellSignaling,4967 HRP-conjugated

(6) Photobiomodulation mitigates chondrocyte catabolism in osteoarthritis by modulating macrophage M1 polarization through the IL-6/JAK/STAT pathwayJ Orthop Surg ResDecember 24, 2025Haiming Wang, Wenhua Ning, Yiying Liu, Jing Nie, Shenao Du, Haowei Zhangincubated overnight at 4 °C with the following primary antibodies diluted at 1:1000: anti-IL-6 (Cat. No. 12153, Cell Signaling Technology, USA), anti-JAK1 (Cat. No. 3344, Cell Signaling Technology, USA), anti-JAK2

(7) Cirsiliol alleviates diabetic cardiomyopathy by inhibiting oxidative stress and improving energy metabolism through the PPAR-α/AMPK pathwayScientific ReportsDecember 11, 2025Jing Tao, Siqi Liu, Yunzhi Ling, Hanlin Bai, Lei Liu, Ru Yu, Guangling Li(Catalog # 5023), Bcl-2 (Catalog # 3498), SOD1 (Catalog # 37385), SOD2 (Catalog # 13141), IL-6 (Catalog # 12153), MCP-1 (Catalog # 2029), PPAR-α (Catalog # 2435), AMPK (Catalog # 5831), LKB1 (Catalog # 3047), and

(8) Biochemical, histopathological, and immunohistochemical study on the ameliorative effect of crocin against lipopolysaccharide‑induced hippocampal toxicity in male albino ratsBMC Pharmacology and ToxicologyNovember 25, 2025Eatemad A. Awadalla, Ola Mohamed, Ahmed Abdelsadik, Hoda S. Sherkawy, Abd El-Kader M. Abd El-Kadersupplemented with 0.3% Triton™ X-100, pH 7.2) for caspase-3 (ab4051) and for IL-6 (Cell Signaling, 12153 S) was applied to each section and incubated overnight in a humidified chamber at 4 °C. Then, after

(9) Bovine Mastitis-Derived Bacillus cereus in Inner Mongolia: Strain Characterization, Virulence Factor Identification, and Pathogenicity ValidationVeterinary SciencesNovember 3, 2025Chen Yu, Kollie Helena Vivian, Shuangyuan Fan, Xiaojiao He, Jingwen Zhao, Zhangping Yang, Kai Zhang, Tianle XuIL-8 were purchased from Cell Signaling Technology (Danvers, MA, USA; #3033, #8242, #6945, #14358, #12153, #94407), and were diluted 1:1000 for incubation. 2.9. Statistics Multiple comparisons among groups

(10) Ubiquitination-activated TAB–TAK1–IKK–NF-κB axis modulates gene expression for cell survival in the lysosomal damage responseeLifeSeptember 24, 2025Akinori Endo, Chikage Takahashi, Naoko Ishibashi, Yasumasa Nishito, Koji Yamano, Keiji Tanaka, Yukiko Yoshidamonoclonal (Cell Signaling Technology, 12242), anti-IL6 rabbit monoclonal (Cell Signaling Technology, 12153), anti-IRF1 rabbit monoclonal (Cell Signaling Technology, 8478), anti-NKX3.1 rabbit monoclonal (Cell Signaling Technology

(11) IL-6 trans-signaling in cystic fibrosis bronchial cells potentiates TNF-α-driven ICAM-1 expressionFrontiers in Cell and Developmental BiologySeptember 23, 2025John Lin, Lyvia Fourcade, Lucie Roussel, Matthew Marabella, Julie Bérubé, Dao Nguyen, Simon Rousseauanti-STAT-3 (D3Z2G) (Cell Signaling, 9132), 1:1,000; and monoclonal rabbit anti-IL-6 (D3K2N) (Cell Signaling, 12153), 1:1,000; these antibodies were diluted in 1% BSA/TBS-Tween and incubated at ambient temperature for

(12) Lipoprotein(a)/CD36 Interaction Drives IL-6/RhoA-GTP Signaling and miRNA Epigenetic Regulation in Coronary Artery SpasmPharmaceuticals (Basel)September 16, 2025Yen-Kuang Lin, Tsung-Han Hsieh, Chi-Tai Yeh, Vijesh Kumar Yadav, Iat-Hang Fong, Kuang-Tai Kuo, Nicholas G. Kounis, Patrick Hu, Ming-Yow HungSignaling Technology, Danvers, MA, USA; Cat #74002, Lot #CD362031), IL-6 (Cell Signaling Technology, Cat #12153, Lot #IL61213), TNF-α (Cell Signaling Technology, Cat #3707, Lot #TNF7081), NF-κB (Cell Signaling Technology,

(13) Verteporfin Mitigates Isoproterenol‐Induced Myocardial Hypertrophy by Attenuating IL‐6/STAT3 in Cardiac FibroblastsCardiovascular TherapeuticsAugust 28, 2025Sunpeng Sheng, Lu Ding, Jinbiao Lai, Zelei Ye, Ru Zhao, Shijia Chen, Jianshe Ma, Junming Fan, Peifeng Jinother reagents used in this study were of analytical grade. Antibodies against YAP (#8418), IL-6 (#12153), p-STAT3 (#9145), STAT3 (#12640), GAPDH (#5174), and secondary antibodies were obtained from Cell Signaling Technology

(14) WSTF nuclear autophagy regulates chronic but not acute inflammationNatureAugust 21, 2025Yu Wang, Vinay V. Eapen, Yaosi Liang, Athanasios Kournoutis, Marc Samuel Sherman, Yanxin Xu, Angelique Onorati, Xianting Li, Xiaoting Zhou, Kathleen E. Corey, et al.(BD Biosciences, G175–405, 1:1000), p-ATM (Abcam, ab81292, 1:1000), IL6 (Cell Signaling Technology, 12153 for human, 1:1000; 12912 for mouse, 1:1000), NF-κB p65 (Cell Signaling Technology, 8242S, 1:1000 and

(15) Avenanthramide-C Mitigates High-Fat Diet-Accelerated Alzheimer’s Pathologies via NOD1-Driven Neuroinflammation in 5×FAD MiceNutrientsAugust 19, 2025Ming Wang, Baoyuan Jin, Jia Xu, Chuang WangCell Signaling), TNF-α (#11948, D2D4, Cell Signaling), IL-1β (#12507, D3H1Z, Cell Signaling), IL-6 (#12153, D3K2N, Cell Signaling), IL-10 (#12163, D13A11, Cell Signaling), NOD1 (#3545, polyclonal antibody, Cell

(16) HJP 272, an endothelin receptor antagonist, and its role in cancer cell migration and invasionTransl OncolAugust 5, 2025Nabeela Baig, Rameswari Chilamakuri, Saurabh Agarwal, Aaron Muth, Sandra E. Reznik#9212S), CDK6 (Santa Cruz # sc-53638), α/β-Tubulin (Cell Signaling #2148S), IL-6 (Cell Signaling # 12153S), Anti-mouse IgG, HRP-linked antibody (Cell Signaling #7076S), Anti-rabbit IgG, HRP-linked antibody

(17) Human Brain Organoids: A New Model to Study Cryptococcus neoformans NeurotropismJ Fungi (Basel)July 19, 2025Alfred T. Harding, Lee Gehrke, Jatin M. Vyas, Hannah Brown Harding(TBST). To detect the CXCL10 (Cell Signaling 14969), CCL5 (Cell Signaling 2988), and IL-6 (Cell Signaling 12153) proteins, blots were incubated for overnight at 4 °C in PBST, 1% BSA and primary antibody (1:1000).

(18) Integrated network pharmacology, transcriptomics, and experimental validation to explore the mechanism of Qihuang Jianpi Zishen granules against Sjögren's syndromeSci RepJuly 1, 2025Haikun Wang, Zichuang Ma, Na Wu, Xin Wang, Xinmeng Yangsynthesized by GeneralBiol (Chuzhou, China). Antibodies targeting TNF-α (batch No: 6945 S), IL-6 (batch No: 12153 S), and IL-1β (batch No: 12703 S) were acquired from Cell Signaling Technology (Danvers, MA, USA). Polyvinylidene

(19) Functional regulation of macrophages by Ces1d-mediated lipid signaling in immunometabolismMolecular metabolismJuly 1, 2025Shao LJ, Elizondo F, Gao F, Habib R, Li X, Pham K, Ysaguirre J, Elizondo M, Shirazi S, Eckel-Mahan KL, et al.Cell Signaling Technology), TNFα (#372, Cell Signaling Technology), IL1β (#12703, Cell Signaling Technology), IL6 (#12153, Cell Signaling Technology), BIP (#PA1-014A, Thermo Fisher Scientific), CGI58 (#sc-365278, Santa Cruz Biotechnology

(20) Tanshinone IIA Regulates NRF2/NLRP3 Signal Pathway to Restrain Oxidative Stress and Inflammation in Uric Acid-Induced HK-2 Fibrotic ModelsEndocrine, Metabolic & Immune Disorders - Drug TargetsJuly 1, 2025Weiliang Zhang, Jiashu Feng, Ruiqi Liu, Ting Xiang, Xinlin WuASC (67824S), Caspase-1 (3866S), Keap1 (8047S), NRF2 (12721S), TNF-α (3707s), IL-1β (12703S), IL-6 (12153S), and IL-18 (67775S) were purchased from Cell Signaling Technology (Shanghai, China). TGF-beta (ab215715,

Write a Review

(1)

7/21/2022Great IL-6 (D3K2N) Rabbit mAb Ines RThis antibody was used to detect IL-6 levels from human cell lysates (25 ug/mL protein loading) through western blot analysis.
(1)

5/11/2022Good but Slightly Faint Antibody for WB Stephanie BaringerUsed this antibody on human astrocytes at 1:1000 dilution. Resulted in a number of fairly weak bands.
(1)

11/4/2016Excellent IL-6 Antibody From Cell Signaling Chandan KumarPresent study was designed to test the effect of different treatment on tissue specific IL-6 levels in diabetic rats. Proteins from rat aorta, liver, muscle, heart, Kidney and retina were extracted ... Read Review

IL-6 (D3K2N) Rabbit mAb

IL-6 (D3K2N) Rabbit mAb from Cell Signaling Technology

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IL-6 (D3K2N) Rabbit mAb from Cell Signaling Technology

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