IL-6 (D3K2N) Rabbit mAb 12153S from Cell Signaling Technology

Product Specs
ItemIL-6 (D3K2N) Rabbit mAb
CompanyCell Signaling Technology
Price $359.00 Inquire
Catalog Number12153S
Quantity100 µl
ApplicationsW, W-S, IP
ReactivityH
ClonalityMonoclonal
TargetIL6
HostRabbit
NCBI Full Gene Nameinterleukin 6
NCBI Gene AliasesBSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6
Research AreaImmunology and Inflammation
CloneD3K2N
Molecular Weight21-28

Questions and Answers

Can this IL-6 antibody be used to study inflammatory responses in human cell lines?

Yes, the IL-6 (D3K2N) Rabbit mAb has been shown to effectively detect IL-6 levels in various studies involving human cell lines. For instance, it was utilized in Western blotting to assess IL-6 expression in response to inflammatory stimuli, demonstrating its applicability in studying inflammatory responses in human cells.

Is this antibody suitable for use in immunoprecipitation experiments?

The IL-6 (D3K2N) Rabbit mAb is suitable for immunoprecipitation (IP) applications, as indicated by its documented use in scientific literature. This allows researchers to isolate IL-6 and study its interactions with other proteins, which is crucial for understanding its role in various biological processes.

How has this antibody been utilized in research related to cancer or inflammation?

The IL-6 antibody has been cited in multiple studies focusing on inflammation and cancer. For example, it was used to measure IL-6 levels in the context of tumor microenvironments, highlighting its role in promoting tumor growth and inflammation, as seen in research on diffuse intrinsic pontine glioma (DIPG) and other cancer models.

What is the molecular weight of the IL-6 protein detected by this antibody?

The IL-6 protein recognized by the IL-6 (D3K2N) Rabbit mAb has a molecular weight ranging from 21 to 28 kDa. This information is essential for researchers to confirm the specificity of their Western blot results when analyzing IL-6 expression.

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Bladder Cancer Cells Maintain Paracrine IL-1 Signaling and IL-1Ra Sensitivity Following Chronic IL-1 ExposureCellsJuly 30, 2026Jessica Gomez, Meron Lakew, Haley Wilkie, Bernice David, Oluwatamilore Taiwo, Roopal Dhar, Anusha Akula, Akshaykumar Thasma, Jeffrey Cho, Neil Sharma, et al.(Cell Signaling Technology, Danvers, MA, USA; 24376T), IL-6 (Cell Signaling Technology, Danvers, MA, USA; 12153S), IL-8 (Cell Signaling Technology, Danvers, MA, USA; 94407S), AR (Cell Signaling Technology, Danvers,

(2) Myeloid MMP14 couples extracellular proteolysis to inflammatory and metabolic remodeling during obesityCell ReportsJuly 28, 2026Long J. Shao, Fathima Elizondo, Feng Gao, Elizabeth L. Lieu, Bharati Reddi, Maryam Elizondo, Iqbal Mahmud, Kristin Eckel-Mahan, Philipp E. Scherer, Xin Ge, et al.1:2500), TNFα (Cell Signaling 372, 1:2500), IL-1β (Cell Signaling 12703, 1:2500), IL-6 (Cell Signaling 12153, 1:2500), and β-actin (BD Biosciences 61256, 1:1000). Signals were detected using IRDye secondary antibodies

(3) Iron overload triggers pathological remodeling of corneal stroma through ferroptosis and senescence-associated secretory phenotype in keratoconusRedox BiologyJuly 1, 2026Xiaoxue Liu, Shengqian Dou, Chaoqun Wei, Ting Liu, Xiaowen Zhang, Lei Zhang, Longfei Zhao, Wenlong Li, Qingdong Bao, Hua GaoMMP3,Proteintech,17873-1-AP,1:1000 Rabbit TNF-α,Cell Signaling Technology,6945,1:1000 Rabbit IL-6,Cell Signaling Technology,12153,1:1000 Rabbit IL-1β,Cell Signaling Technology,12703,1:1000 Rabbit FTL,Proteintech,10727-1-AP,1:6000 Rabbit

(4) Salidroside alleviates TNF-α-induced endothelial inflammatory injury by modulating NF-κB/NLRP3 inflammasome-related signaling: an integrated network pharmacology and experimental studyTranslational PediatricsJune 30, 2026Jiahui Meng, Weiwei Li, Lan He, Guoying Huang, Fang LiuCST, Danvers, MA, USA), phospho-NF-kB p65 (1:1,000 dilution, 3031SS, CST), IL-6 (1:1,000 dilution, 12153S, CST), NLRP3 (1:1,000 dilution, 15101S, CST), GSDMD (1:1,000 dilution, 39754T, CST), Caspase-1 (1:2,000

(5) MiR-766-3P promotes degenerative lumbar disc disease by regulating SIRT6 to aggravate the inflammatory response of human nucleus pulposus cellsHereditasJune 3, 2026Dongwei An, Pan YangUSA), IL-1β monoclonal antibody (#12242; Cell Signaling Technology, USA), IL-6 monoclonal antibody (#12153; Cell Signaling Technology, USA), aggrecan monoclonal antibody (#28971; Cell Signaling Technology, USA),

(6) Red ginseng-derived components attenuate UVB-induced keratinocyte senescence via senomorphic mechanismsJournal of Ginseng ResearchMay 1, 2026Sung Ha Lim, Hee-Seok Seo, Hyun Kang, Seung-Phil Hong1:1000, 16 kDa), anti-phospho-Histone H2A.X (Ser139; γH2A.X; CST #9718, 1:1000, 15 kDa), anti-IL-6 (CST #12153, 1:1000, 21 kDa), and anti-β-actin (Santa Cruz Biotechnology SC-47778, 1:4000, 43 kDa), which was used

(7) Subclonal β-catenin/YAP signaling heterogeneity accelerates ovarian cancer metastasis through a senescence-associated secretory phenotypeCell Death & DiseaseApril 23, 2026Sally K. Y. To, Yibin Yang, Zeyu Shi, Katie S. W. Fung, Sook Ling Lai, Joshua J. X. Li, Philip P. C. Ip, Alice S. T. Wong(#8480), total YAP (#14074), phospho-YAP (S127) (#13008), phospho-YAP (S397) (#13619), p21 (#2947), IL-6 (#12153), and PAI-1 (#11907) (all from Cell Signaling Technology), and β-actin (Sigma, #A5441). Blots were imaged

(8) Schisandrin B suppresses cholangiocarcinoma by targeting the ROS/p38 MAPK/NF‑κB axisOncology LettersMarch 26, 2026Junhao Yang, Wenying Long, Xiaoxiao Wu, Xiaohui Yang, Qiang Hong, Shuai Wang(Ser32),2859,"1:1,000","Cell Signaling Technology, Inc." IκBα,4814,"1:1,000","Cell Signaling Technology, Inc." IL-6,12153,"1:1,000","Cell Signaling Technology, Inc." IL-8,94407,"1:1,000","Cell Signaling Technology, Inc." TNF-α,6945,"1:1,000","Cell Signaling Technology

(9) Integrative biology shows DPP4 affects inflammatory response to eclampsia and cell model growth via p65/NLRP3/ASC/Caspase-1 pathwayFront GenetMarch 4, 2026Zhimin Bian, Li Hao, Rongjuan Yang, Jianghui Sunprimary antibodies: DPP4 (Abcam, Catalog No. ab133605, UK), IL6 (Cell Signaling Technology, Catalog No. 12153, United States), TNF-α (Cell Signaling Technology, Catalog No. 3707, USA), GAPDH (Cell Signaling Technology,

(10) Osimertinib activates a TGF-β2-dependent secretory program that drives lung adenocarcinoma progressionJournal of Clinical InvestigationFebruary 2, 2026Madhurima Ghosh, Chao Wu, Abhishek Kumar, Monique B. Nilsson, John V. Heymach, Weina Zhao, Jiang Yu, Xin Liu, Na Ding, Shike Wang, et al.(catalog 4879S), GM130 (catalog 12480S), β-actin (catalog 4970S), p65 (catalog 8242T), IL-6 (catalog 12153), p-STAT3 (catalog 9131S), STAT-3 (catalog 9132) from Cell Signaling Technology; primary antibodies

(11) Metformin inhibits nuclear egress of chromatin fragments in senescence and agingNature AgingJanuary 16, 2026Takuya Kumazawa, Yanxin Xu, Yu Wang, Ji-Won Lee, Tara C. O’Brien, Chia-Kang Ho, Murat Cetinbas, Aaron Weiner, Konrad Hochedlinger, Ruslan I. Sadreyev, et al.was used for Extended Data Fig. 9a), p16 (BD Biosciences G175-405), IL6 (Cell Signaling Technology 12153), IL8 (Abcam ab18672), cGAS (Cell Signaling Technology 15102), STING (Cell Signaling Technology 13647),

(12) Piezo1 knockdown activates PI3K/AKT and enhances SPP1 to drive M2 macrophage polarization and reduce cardiac inflammationScientific ReportsJanuary 8, 2026Yunhan Zhang, Ying Zhang, Jiaoyan Song, Ziwen Zhao, Wenhao Ju, Hao Zhang, Shuang Li, Hanlu Li, Qiuzhe Guo, Yinbo Ma, et al.(Proteintech, 15939-1-AP, 1:500), IL-1β (Proteintech, 26048-1-AP, 1:1000), IL-6 (Cell Signaling Technology, 12153, 1:1000), IL-10RA (Proteintech, 13356-1-AP, 1:1000), Phospho-Akt (Cell Signaling Technology, 5012S,

(13) Targeting Tris(2,3-dibromopropyl) Isocyanurate-Induced Inflammation in Hippocampal Neurons In Vitro: Mechanistic Insights and Implications for Neurodegenerative Disease PreventionMolecular NeurobiologyJanuary 1, 2026Dominika Szlachcikowska, Oliwia Koszła, Przemysław Sołek, Anna Tabęcka-Łonczyńskas,Rabbit,1:1000,CellSignaling,40435 Primary,Anti-p-eIF2α,Rabbit,1:1000,CellSignaling,3398 Primary,Anti-ATF4,Rabbit,1:1000,CellSignaling,11815 Primary,Anti-Nrf2,Rabbit,1:1000,CellSignaling,12721 Primary,Anti-Keap1,Rabbit,1:1000,CellSignaling,8047 Primary,Anti-HO-1,Rabbit,1:1000,CellSignaling,43966 Primary,Anti-NQO1,Rabbit,1:1000,CellSignaling,62262 Primary,Anti-TNF-α,Rabbit,1:1000,CellSignaling,8184 Primary,Anti-IL-1β,Rabbit,1:1000,CellSignaling,12703 Primary,Anti-IL6,Rabbit,1:1000,CellSignaling,12153 Primary,Anti-GAPDH,Mouse,1:100 000,ABClonal,AC033 ,Anti-β-actin,Rabbit,1:1000,CellSignaling,4967 HRP-conjugated

(14) Photobiomodulation mitigates chondrocyte catabolism in osteoarthritis by modulating macrophage M1 polarization through the IL-6/JAK/STAT pathwayJournal of orthopaedic surgery and researchDecember 24, 2025Wang H, Ning W, Liu Y, Nie J, Du S, Zhang H.incubated overnight at 4 °C with the following primary antibodies diluted at 1:1000: anti-IL-6 (Cat. No. 12153, Cell Signaling Technology, USA), anti-JAK1 (Cat. No. 3344, Cell Signaling Technology, USA), anti-JAK2

(15) Cirsiliol alleviates diabetic cardiomyopathy by inhibiting oxidative stress and improving energy metabolism through the PPAR-α/AMPK pathwayScientific ReportsDecember 11, 2025Jing Tao, Siqi Liu, Yunzhi Ling, Hanlin Bai, Lei Liu, Ru Yu, Guangling Li(Catalog # 5023), Bcl-2 (Catalog # 3498), SOD1 (Catalog # 37385), SOD2 (Catalog # 13141), IL-6 (Catalog # 12153), MCP-1 (Catalog # 2029), PPAR-α (Catalog # 2435), AMPK (Catalog # 5831), LKB1 (Catalog # 3047), and

(16) Osimertinib activates a TGF-β2–dependent secretory program that drives lung adenocarcinoma progressionJ Clin InvestDecember 9, 2025Madhurima Ghosh, Chao Wu, Abhishek Kumar, Monique Nilsson, John V. Heymach, Weina Zhao, Jiang Yu, Xin Liu, Na Ding, Shike Wang, et al.(catalog 4879S), GM130 (catalog 12480S), β-actin (catalog 4970S), p65 (catalog 8242T), IL-6 (catalog 12153), p-STAT3 (catalog 9131S), STAT-3 (catalog 9132) from Cell Signaling Technology; primary antibodies

(17) Biochemical, histopathological, and immunohistochemical study on the ameliorative effect of crocin against lipopolysaccharide‑induced hippocampal toxicity in male albino ratsBMC Pharmacology and ToxicologyNovember 25, 2025Eatemad A. Awadalla, Ola Mohamed, Ahmed Abdelsadik, Hoda S. Sherkawy, Abd El-Kader M. Abd El-Kadersupplemented with 0.3% Triton™ X-100, pH 7.2) for caspase-3 (ab4051) and for IL-6 (Cell Signaling, 12153 S) was applied to each section and incubated overnight in a humidified chamber at 4 °C. Then, after

(18) Bovine Mastitis-Derived Bacillus cereus in Inner Mongolia: Strain Characterization, Virulence Factor Identification, and Pathogenicity ValidationVeterinary SciencesNovember 3, 2025Chen Yu, Kollie Helena Vivian, Shuangyuan Fan, Xiaojiao He, Jingwen Zhao, Zhangping Yang, Kai Zhang, Tianle XuIL-8 were purchased from Cell Signaling Technology (Danvers, MA, USA; #3033, #8242, #6945, #14358, #12153, #94407), and were diluted 1:1000 for incubation. 2.9. Statistics Multiple comparisons among groups

(19) Ubiquitination-activated TAB–TAK1–IKK–NF-κB axis modulates gene expression for cell survival in the lysosomal damage responseeLifeSeptember 24, 2025Akinori Endo, Chikage Takahashi, Naoko Ishibashi, Yasumasa Nishito, Koji Yamano, Keiji Tanaka, Yukiko Yoshidamonoclonal (Cell Signaling Technology, 12242), anti-IL6 rabbit monoclonal (Cell Signaling Technology, 12153), anti-IRF1 rabbit monoclonal (Cell Signaling Technology, 8478), anti-NKX3.1 rabbit monoclonal (Cell Signaling Technology

(20) Ubiquitination-activated TAB–TAK1–IKK–NF-κB axis modulates gene expression for cell survival in the lysosomal damage responseElifeSeptember 24, 2025Akinori Endo, Chikage Takahashi, Naoko Ishibashi, Yasumasa Nishito, Koji Yamano, Keiji Tanaka, Yukiko Yoshidamonoclonal (Cell Signaling Technology, 12242), anti-IL6 rabbit monoclonal (Cell Signaling Technology, 12153), anti-IRF1 rabbit monoclonal (Cell Signaling Technology, 8478), anti-NKX3.1 rabbit monoclonal (Cell Signaling Technology

Write a Review

(1)

7/21/2022Great IL-6 (D3K2N) Rabbit mAb Ines RThis antibody was used to detect IL-6 levels from human cell lysates (25 ug/mL protein loading) through western blot analysis.
(1)

5/11/2022Good but Slightly Faint Antibody for WB Stephanie BaringerUsed this antibody on human astrocytes at 1:1000 dilution. Resulted in a number of fairly weak bands.
(1)

11/4/2016Excellent IL-6 Antibody From Cell Signaling Chandan KumarPresent study was designed to test the effect of different treatment on tissue specific IL-6 levels in diabetic rats. Proteins from rat aorta, liver, muscle, heart, Kidney and retina were extracted ... Read Review

IL-6 (D3K2N) Rabbit mAb

IL-6 (D3K2N) Rabbit mAb from Cell Signaling Technology

Description

Questions and Answers

Cell Signaling Technology

Citations (143)

Reviews

IL-6 (D3K2N) Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map