SirT1 (C14H4) Rabbit mAb 2496S from Cell Signaling Technology

Product Specs
ItemSirT1 (C14H4) Rabbit mAb
CompanyCell Signaling Technology
Price $336.00 Inquire
Catalog Number2496S
Quantity100 µl
ApplicationsW, IP
ReactivityH
TargetSirT1
HostRabbit
NCBI Gene AliasesSIR2, SIR2L1, SIR2alpha
ClonalityMonoclonal
NCBI Full Gene Namesirtuin 1
Research AreaChromatin Regulation / Nuclear Function
CloneC14H4
Molecular Weight120

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Effects of Swimming at Different Water Temperatures on Muscle and Adipose Tissue Adaptation in Diet-Induced Obese MiceInternational Journal of Medical SciencesFebruary 11, 2026Tzu-Jung Chou, Chia-Wen Lu, Yi-Ju Hsu, Chi-Chang Huang, Kuo-Chin HuangSDS-PAGE. Membranes were incubated with primary antibodies targeting SIRT1 (Cell signaling, catalog# 2496, 1:1000), PGC-1α (ABclonal, catalog# A12348, 1:1000), FNDC5 (Abcam catalog# ab174833, 1:1000), and β-actin

(2) ENOX2 (tNOX)–Associated Stemness in Oral Cancer Cells and Its Clinical Correlation in Head and Neck TumorsAntioxidants (Basel)January 13, 2026Che-Wei Wang, Atikul Islam, Yu-Tung Shih, Chin-Fang Chang, Mu Kuan Chen, Pin Ju Chuehantibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA): anti-SIRT1 (1:1000, Cat#2496), anti-Nanog (1:2000, Cat#4903), anti-SOX2 (1:1000, Cat#2748), anti-Oct4 (1:1000, Cat#2750), anti-ABCG2

(3) Effects of Nicotinamide Mononucleotide Supplementation and Aerobic Exercise on Metabolic Health and Physical Performance in Aged MiceNutrientsOctober 2, 2025Yi-Ju Hsu, Mon-Chien Lee, Huai-Yu Fan, Yu-Ching Loprotein expression was normalized to GAPDH. The primary antibody used in this study was anti-SirT1 (C14H4) (1:500; #2496; Cell Signaling Technology, Danvers, MA, USA). Protein expression was quantified by densitometric analysis

(4) SIRT7 regulates NUCKS1 chromatin binding to elicit metabolic and inflammatory gene expression in senescence and liver agingMolecular CellJune 19, 2025Khoa A. Tran, Michael Gilbert, Berta N. Vazquez, Alessandro Ianni, Benjamin A. Garcia, Alejandro Vaquero, Shelley L. BergerRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies SIRT7,Cell Signaling,5360S SIRT1,Cell Signaling,2496S SIRT6,Cell Signaling,12486 FLAG,Sigma-Aldrich,F1804 NUCKS1,Sigma-Aldrich,HPA062351-100UL NUCKS1,Bethyl,A301-508A-T NUCKS1,ProteinTech,12023-2-AP NPM1

(5) miRNA-142-3p aggravates hydrogen peroxide-induced human umbilical vein endothelial cell premature senescence by targeting SIRT1Bioscience ReportsMay 29, 2024Pengfei Tong, Jingke Zhang, Shuang Liu, Jiyang An, Gehan Jing, Laifeng Ma, Ruihua Wang, Zhengfeng Wangovernight at 4°C. Primary antibodies included the rabbit monoclonal antibody against SIRT1 (1:1000, 2496, CST), rabbit polyclonal antibody against p21 (1:1000, 381102, ZENBIO), rabbit polyclonal antibody against

(6) MiR-217 Regulates SIRT1 Expression and Promotes Inflammatory and Apoptotic Responses in OsteoarthritisGenesNovember 29, 2023Papageorgiou AA, Roussos A, Papathanasiou I, Balis C, Karachalios T, Varitimidis SE, Malizos KN, Tsezou A.the respective primary antibody, in blocking buffer at 4 °C. Anti-SIRT1 (1:1000, C14H4 Rabbit mAb #2496T; Cell Signaling Technology, Danvers, MA, USA), anti-Acetylated-p53 (anti-Acetyl-p53 (Lys382); 1:500,

(7) BZD9L1 Differentially Regulates Sirtuins in Liver-Derived Cells by Inducing Reactive Oxygen SpeciesBiomedicinesNovember 15, 2023Lee YT, Tan YJ, Oon CE.with the membrane. The primary antibodies were SIRT1 (Cell Signaling Technology, Danvers, MA, USA, 2496S, rabbit polyclonal), SIRT3 (Cell Signaling Technology, 5490, rabbit polyclonal), SIRT4 (Santa Cruz,

(8) Menin orchestrates hepatic glucose and fatty acid uptake via deploying the cellular translocation of SIRT1 and PPARγCell & BioscienceSeptember 22, 2023Tingjun Liu, Ranran Li, Lili Sun, Zhongjin Xu, Shengxuan Wang, Jingxuan Zhou, Xuanning Wu, Kerong Shiused: anti-Menin (Bethyl Laboratories, A300-105A), anti-Pparγ (Bioss, bsm-33436 M), anti-Sirt1 (CST, #2496 T). The following secondary antibodies were used: goat anti-mouse IgG H&L Cy5 (Bioss, 0296G-Cy5), goat

(9) Characterizing 3T3-L1 MBX Adipocyte Cell Differentiation Maintained with Fatty Acids as an In Vitro Model to Study the Effects of ObesityLife (Basel)August 9, 2023Noshin Mubtasim, Lauren Gollahon7389), GCN5L2 Rabbit mAb (Product # 3305), PPAR-γ Rabbit mAb (Product# 2435) SirT1 Rabbit mAb (Product # 2496), RXRα Rabbit mAb (Product# 3085) primary antibodies from Cell Signaling Technology were used at 1:1000

(10) A miR-125/Sirtuin-7 pathway drives the pro-calcific potential of myeloid cells in diabetic vascular diseaseDiabetologiaSeptember 1, 2022Vigili de Kreutzenberg S, Giannella A, Ceolotto G, Faggin E, Cappellari R, Mazzucato M, Fraccaro C, Tarantini G, Avogaro A, Fadini GP.RUNX2 and OSX were determined by western blot (WB) analysis. Antibodies against SIRT1 (catalogue no. 2496), SIRT7 (catalogue no. 5360), RUNX2 (catalogue no. 8486) and GAPDH (catalogue no. 2118) were purchased

(11) Senescent macrophages in the human adipose tissue as a source of inflammagingGeroScienceAugust 1, 2022Matacchione G, Perugini J, Di Mercurio E, Sabbatinelli J, Prattichizzo F, Senzacqua M, Storci G, Dani C, Lezoche G, Guerrieri M, et al.,Cell Signaling Technology/4060 pJNK,M,1:100,Santa Cruz Biotechnology/sc-6254 SIRT1,R,1:1000,Cell Signaling Technology/2496 Primary antibodies *M mouse, R rabbit

(12) Rhein Improves Renal Fibrosis by Restoring Cpt1a-Mediated Fatty Acid Oxidation through SirT1/STAT3/twist1 PathwayMolecules (Basel, Switzerland)April 6, 2022Song X, Du Z, Yao Z, Tang X, Zhang M.anti-p-STAT3 antibody (#9145), rabbit anti-acetylated-Lysine (#9441), rabbit anti-SirT1 antibody (#2496), FITC-goat anti-rabbit IgG (#4414) and Alexa-goat anti-mouse IgG (#4412) were purchased from Cell Signaling Technology

(13) Role of sirtuin 1 in the brain development in congenital hypothyroidism rats via the regulation of p53 signaling pathwayBioengineeredApril 1, 2022Wei X, Tan J, Gao H.skimmed milk for 1 h. Subsequently, the membrane was incubated with antibodies against SIRT1 (cat. no. 2496; dilution 1: 1000; Cell Signaling Technology, Inc.), p53 (cat. no. 2527; dilution 1: 1000; Cell Signaling Technology

(14) Molecular Fingerprint of BMD Patients Lacking a Portion in the Rod Domain of DystrophinInternational journal of molecular sciencesFebruary 27, 2022Capitanio D, Moriggi M, Barbacini P, Torretta E, Moroni I, Blasevich F, Morandi L, Mora M, Gelfi C.TX, USA, sc-576, 1:500), rabbit monoclonal anti-SirT1 (Cell Signaling Technology, Danvers, MA, USA, #2496, 1:1000) and anti-SirT2 (Cell Signaling Technology, #12650, 1:1000). After washing, membranes were incubated

(15) Protective effect of liquiritin on coronary heart disease through regulating the proliferation of human vascular smooth muscle cells via upregulation of sirtuin1BioengineeredFebruary 1, 2022Liang Yuan, Dajie Wang, Chunyang WuCell Signaling Technology, Inc.), MMP9 (cat. no. 13,667; 1: 1000; Cell Signaling Technology, Inc.), SIRT1 (cat. no. 2496; 1: 1000; Cell Signaling Technology, Inc.), or GAPDH (cat. no. 5174; 1: 1000; Cell Signaling Technology,

(16) MiRNA-124-3p.1 sensitizes hepatocellular carcinoma cells to sorafenib by regulating FOXO3a by targeting AKT2 and SIRT1Cell Death & DiseaseJanuary 10, 2022Zhe-bin Dong, Heng-miao Wu, Yi-cheng He, Zhong-ting Huang, Yi-hui Weng, Hong Li, Chao Liang, Wei-ming Yu, Wei Chen12829S), p-FOXO3a (Ser253, 9466S), total-AKT2 (t-AKT2, 3063S), p-AKT2 (Ser473, 4060S), total-SIRT1 (2496S), acetylated-lysine (9441S), IgG (2985S), caspase-3 (14220S), and cleaved-caspase-3 (9661S) in Western blot

(17) Dual Role of SIRT1 in Autophagy and Lipid Metabolism Regulation in Osteoarthritic ChondrocytesMedicina (Kaunas, Lithuania)November 4, 2021Papageorgiou AA, Goutas A, Trachana V, Tsezou A.blocking buffer at 4 °C. The antibodies used were anti-SIRT1 (1:1000, Cell Signaling Technology, Rabbit #2496), anti-LC3 (1:1000 dilution, Cell Signaling Technology, LC3A/B Rabbit #4108), p62 (1:1000 dilution,

(18) Hsp22 Deficiency Induces Age-Dependent Cardiac Dilation and Dysfunction by Impairing Autophagy, Metabolism, and Oxidative ResponseAntioxidants (Basel, Switzerland)September 29, 2021Wu W, Sun X, Shi X, Lai L, Wang C, Xie M, Qin G, Qiu H.,Cell signaling,#2532,1:5000 CPT1a,WB,Abcam,ab234111,"1:10,000" CPT2,WB,Abcam,ab181114,"1:10,000" Lipin1,WB,Cell signaling,#14906,1:2000 SirT1,WB,Cell signaling,#2496,1:2000 PGC1α,WB,Invitrogen,PA5-38021,"1:10,000" PPAR γ,WB,Santa cruz,sc-7196,1:2000 BAG3,WB,Proteintech,10599-1-AP,1:5000 Hsp70,WB,Cell signaling

(19) Interactome Networks of FOSL1 and FOSL2 in Human Th17 CellsACS omegaSeptember 28, 2021Shetty A, Bhosale SD, Tripathi SK, Buchacher T, Biradar R, Rasool O, Moulder R, Galande S, Lahesmaa R.anti-JUNB C-11 (Santa Cruz Biotechnology, cat no. sc-8051); anti-SIRT1 (Cell Signaling Technology, cat no. 2496); and anti-JUN (BD Biosciences, cat no. 610326). Conformation-specific rabbit HRP (Cell Signaling Technology,

(20) Metformin acts to suppress β-hydroxybutyric acid-mediated inflammatory responses through activation of AMPK signaling in bovine hepatocytesJournal of Animal ScienceJuly 1, 2021Tianle Xu, Xubin Lu, Abdelaziz Adam Idriss Arbab, Xinyue Wu, Yongjiang Mao, Juan J Loor, Zhangping Yang(Danvers, MA) (#3033, #15101, #3866, #7627, #9649S, #4499, #2535, #5831, #11818, #3676, #12282S, and # 2496S), and p65 was purchased from Abcam (ab16502) and diluted 1:1,000 for incubation. The primary antibody

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10/6/2016SIRT1 Western Blot Using Primary CD4+ T-Cells Max HübnerHere, we detected expression levels of SIRT1 protein in primary human CD4+ T-Cells before and after Treg differentiation.

SirT1 (C14H4) Rabbit mAb

SirT1 (C14H4) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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SirT1 (C14H4) Rabbit mAb from Cell Signaling Technology

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