Insulin (C27C9) Rabbit mAb 3014S from Cell Signaling Technology

Product Specs
ItemInsulin (C27C9) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number3014S
Quantity100 µl
ApplicationsW-S, IHC-P, IF-F, IF-IC, FC-FP
ReactivityH, M, R
Targetinsulin
HostRabbit
NCBI Gene AliasesIDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, PNDM4
ClonalityMonoclonal
NCBI Full Gene Nameinsulin
Research AreaMetabolism
IsotypeIgG
CloneC27C9

Questions and Answers

What species can I use this insulin antibody on for my experiments?

The Insulin (C27C9) Rabbit mAb is reactive with mouse, human (Homo sapiens), and rat species, making it suitable for a variety of studies involving these organisms.

Has this antibody been used in any specific experimental applications?

Yes, the Insulin (C27C9) Rabbit mAb has been shown to work in applications such as Western Blotting, Immunofluorescence, Immunocytochemistry, and Immunohistochemistry, as evidenced by its use in multiple scientific studies.

Can this antibody help in studying pancreatic beta-cell function?

Indeed, the Insulin (C27C9) Rabbit mAb has been utilized in research focusing on pancreatic beta-cell function, including studies that investigate the effects of various treatments on insulin production and secretion in models of diabetes, such as the NRG-Akita mice model.

What is the dilution recommended for using this antibody in immunohistochemistry?

For immunohistochemistry applications, the recommended dilution for the Insulin (C27C9) Rabbit mAb is typically 1:250, as noted in various studies that employed this antibody for staining pancreatic tissues.

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Website: Technical Support

(1) TBL1X/TBL1XR1 govern β-cell identity through a PAX6-containing gene regulatory networkNat CommunApril 23, 2026Alina A. Walth-Hummel, Celine Jouffe, Peter Weber, Karsten Motzler, Julia Geppert, Michael Sterr, Wei Gan, Iwona Szczerbinska, Ann-Christine König, Stefanie M. Hauck, et al.µm consecutive slices using the following antibodies: anti-Insulin, Cell Signaling Technology, Cat# 3014; RRID:AB_2126503; anti-Glucagon, Takara Bio, Cat# M182; RRID:AB_2619627, goat anti-Rabbit IgG (H + L)

(2) Intravenous rAAV9 Produces Time-Resolved Parenchymal Labeling Downstream of the Vasculature in Adult MiceBiology (Basel)March 5, 2026Alejandro Soto-Avellaneda, Anton D. Pugel, Jocelyn R. Holmes, Alyssa M. Hicks, Sara Z. Alsaifi, Gyandarshika Koirala, Alexandra E. Oxford, Brad E. Morrison1:100), anti-NeuN (Cell Signaling Technology; 24307S; 1:100), anti-insulin (Cell Signaling Technology; 3014S; 1:800), anti-glucagon (Santa Cruz Biotechnology, Dallas, TX, USA; sc-514592, 1:100), anti-calbindin

(3) Loss of Beta-Cell Identity and Function as a Mechanism of Secondary Failure of Sulfonylurea Therapy in DiabetesMedComm (2020)February 9, 2026Sumit Patel, Zihan Yan, Maria S. Remediand incubated overnight in primary antibodies against insulin (1:100; Cell Signaling Technology; cat# 3014), glucagon (1:100; Abcam; cat# Ab10988), Sst (1:200; Proteintech; cat# 17512‐1‐AP), Nkx6.1 (1:400; Novus Biological

(4) Islet regeneration protein Reg3g promotes macrophage clearance of β cell-derived dysfunctional mitochondria-rich vesicles to mitigate T2DMRedox BiologyFebruary 1, 2026Senlin Li, Mengheng Wang, Hong Zhou, Jia Liu, Mengxuan Wang, Huimin Yuan, Mengzhu Zheng, Xin Li, Xiangling Zhu, Ming Xiangfor 1 h and blocked with 10 % goat serum, followed by incubation with primary antibodies Insulin (#3014, CST), Glucagon (sc-514592, Santa Cruz), CD11c (#97585, CST), CD206 (#24595, CST) at 4 °C overnight.

(5) SerpinA3k Deficiency Ameliorates Experimental type 2 DiabetesCell Mol Life SciDecember 2, 2025Isaac González-Soria, Esmeralda Palacios-Brito, Dulce Gómez-Trujillo, Karla Barragán-Jiménez, Axel D. Soto-Valadez, Miguel Angel Martínez-Rojas, Daniel Oliva-García, Jorge Aranda, Rosalba Pérez-Villalva, Lorena López-Griego, et al.Slides were incubated with primary anti-insulin antibody for 45 min (1:500, Cell Signaling, Cat. No. #3014) and then incubated with the HRP Multimer for 15 min. The reaction was stopped after 1 min and counterstaining

(6) A Small-Molecule Mitofusin 1 Agonist Enhances Islet Survival Under Hypoxic Conditions In Vitro and Improves Transplantation OutcomesBiomoleculesNovember 11, 2025Yue Wang, Bofeng Yang, Pengkun Song, Zexiang Ji, Di Zhang, Wenxuan Chen, Lei Du, Lei Liu0.5% Triton X-100 for 20 min. Primary antibodies against insulin (1:1000, Cell Signaling Technology, 3014, Danvers, MA, USA), the mitochondrial marker Tomm20 (1:2000, Proteintech, 11802-1-AP), and chromogranin

(7) O-glycosylated peptides curtail the aggregation and toxicity of human islet amyloid polypeptideCommunications BiologyNovember 6, 2025Qian Zhang, Xiaohan Mei, Yan Li, Ziyang Wang, Ang Li, Dantong Liu, Sunil Kumar, Deling Kong, Andrew D. Hamilton, Chunqiu Zhangtemperature for 20 min. Subsequently, the samples were incubated with rabbit anti-insulin antibody (Cat # 3014, Cell Signaling Technology, Shanghai, China) at 4 °C overnight (1:200), followed by incubation with

(8) Constructing chimeric mouse islets to study alpha- and delta-cell influence on beta-cell featureMolecular MetabolismNovember 1, 2025Alexis Fouque, Masaya Oshima, Nina Mode, Romain Ducellier, Delphine Thibaut, Florence Gbahou, Latif Rachdi, Over Cabrera, Raphaël Scharfmann(1:1,000, Cell Signaling, #5364L), RPS6 (1:1,000, Cell Signaling, #2217S), INS (1:1,000, Cell Signaling, #3014T), puromycin (1:1,000, Sigma–Aldrich, #MABE343), tubulin (1:1,000, Sigma–Aldrich, #T9026), anti-mouse

(9) MODY PDX1P33T: a mouse model reveals phenotypic divergence from human diseaseFront Endocrinol (Lausanne)October 22, 2025Aliona Harten, Maximilian R. Schmidtke, Florian Giesert, David A. Skerrett-Byrne, Raffaele Teperino, Gerhard K. H. Przemeck, Martin Hrabě de Angelisparaformaldehyde, paraffin-embedded, and sectioned at 3µm. Sections were co-stained for insulin (Cell Signaling #3014; 1:400) and glucagon (Merck G2654; 1:1000) using secondary antibodies AF750 goat anti-rabbit (Invitrogen

(10) Mapping cellular cytosolic pH in vivo under physiological and pathophysiological conditionsJournal of Biological ChemistryOctober 1, 2025Shuwei Zhang, Xiaoxin Hu, Bowen Zhang, Jingxuan Liu, Hexi Feng, Caiying Liu, Yi Hui, Yujiang Fang, Ling Liu, Xiaoqing ZhangAbclonal, A11683), Mist1 (1:200, mouse IgG, Santa Cruz, sc-166181), Insulin (1:2000, rabbit IgG, CST, 3014) and Glucagon (1:2000, rabbit IgG, Abcam, ab92517), CD3 (1:300, mouse IgG, Servicebio, GB12014), CD4

(11) Pharmacologic stimulation of insulin granule acidification increases β-cell zinc content and augments β-cell-targeted drug deliveryJournal of Biological ChemistryOctober 1, 2025Sooyeon Lee, Hannah P. Fraser, Rebecca C. Schugar, Haixia Xu, Timothy M. Horton, Ella A. Thomson, Julie Park, Xucheng Zhang, Justin P. AnnesSignaling Technology; #56791:200), BrdU (Abcam; ab6326; 1:200), insulin (Cell signaling Technology; #3014; 1:500), and Ki-67 (BD Pharmingen; 550609; 1:200)-followed by 1 h incubation in secondary antibodies

(12) A multichaperone condensate enhances protein folding in the endoplasmic reticulumNature Cell BiologySeptember 1, 2025Anna Leder, Guillaume Mas, Viktória Szentgyörgyi, Roman P. Jakob, Timm Maier, Anne Spang, Sebastian Hilleranti-proinsulin (for secretion, 1:1,000; Invitrogen, MA1-83256; lot: 160454; for lysate, 1:2,000; Cell Signaling, 3014; lot: 10) in Can get signal solution (Toyobo) at 4 °C, followed by 2 h incubation with HRP-conjugated

(13) β-cell Gɑs signaling is critical for physiological and pharmacological enhancement of insulin secretionJournal of Clinical InvestigationAugust 15, 2025Megan E. Capozzi, David Bouslov, Ashot Sargsyan, Michelle Y. Chan, Alex Chen, Sarah M. Gray, Katrina Viloria, Akshay Bareja, Jonathan D. Douros, Sophie L. Lewandowski, et al.different depths through the pancreas, and serial sections were stained for insulin (Cell Signaling; 3014S) or Chromogranin A (Thermo Fisher Scientific; PA5-32349). Antibody-positive areas and total pancreas

(14) Hepatic ASPG-mediated lysophosphatidylinositol catabolism impairs insulin signal transductionThe EMBO JournalAugust 4, 2025Feiyan Li, Hua-Sheng Huang, Qingwen Zhao, Wei Zhang, Ting Shi, Wenjing Lv, Qi Zhu, Haojie Liu, Yingjiang Xu, Haiyan Huang, et al.Technology CO., Ltd). The pancreas sections were incubated with anti-glucagon (Cell Signaling Technology #3014 s, 1:10,000) and anti-insulin (Cell Signaling Technology #2760 s, 1:800) antibodies at 4 °C overnight.

(15) The key regulation of LncRNA MALAT during reprogramming of primary mouse hepatocytes into insulin producing cellsSci RepJuly 9, 2025Yunfei Luo, Shuang Liu, Shan Xu, Haixia Zeng, Yuying Zhang, Jin-E Li, Shiqi Yang, Quanwen Liu, Hongbo Xin, Jianping Liu(CK18: Origene, TA500015S, 1:300; ALB: CST, 4746 T, 1:300; PDX1: Abcam, EPR22002, 1:500; Insulin: CST, 3014, 1:300; C-peptide: CST, 4593, 1:300) overnight at 4 °C. Fluorescent dyes iFluo 488 or iFluo 594 were

(16) ACSS2 mediates an epigenetic pathway to regulate β-cell adaptation during gestation in miceNat CommunMay 20, 2025Yu Zhang, Shuang He, Xi Wang, Xin Wang, Mao-Yang He, Xin-Xin Yu, Cheng-Ran Xuwas performed using the ABC Kit (Vector Labs, PK-4000). Antibodies used included anti-insulin (CST, 3014) and biotinylated anti-rabbit IgG (Vector Labs, BA1100). Insulin-positive β-cell area and pancreas area

(17) G Protein Coupled Estrogen Receptor Signaling Maintains β Cell Identity in Female MicebioRxivMay 20, 2025Madeline R. McLaughlin, Preethi Krishnan, Wenting Wu, Cameron Rostron, Kara Orr, Lata Udari, Jacqueline Del Del Carmen Aquino, Amanda Fisher, Tatsuyoshi Kono, Kok Lim Kua, et al.blocking serum (MP-7401A, Vector Labs) for 30 minutes, and then incubated with primary anti-insulin antibody (3014S, Cell Signaling Technology) or primary anti-glucagon antibody (ab92517, Abcam) diluted 1:400 in 2.5%

(18) Immune and Angiogenic Profiling of Mesenchymal Stem Cell Functions in a Subcutaneous Microenvironment for Allogeneic Islet TransplantationAdvanced science (Weinheim, Baden-Wurttemberg, Germany)May 1, 2025Campa-Carranza JN, Capuani S, Joubert AL, Hernandez N, Bo T, Sauceda-Villanueva OI, Conte M, Franco L, Farina M, Rome GE, et al.washed and incubated with a 1:200 dilution of primary anti‐rat insulin antibody (C27C9; Cell Signaling, 3014S) for 4 days. Next, the samples were washed for 3 consecutive incubations of 2 h in PBS. The tissues

(19) PPDPF is not a key regulator of human pancreas developmentPLoS GenetApril 7, 2025Markus Breunig, Meike Hohwieler, Jasmin Haderspeck, Felix von Zweydorf, Natalie Hauff, Lino-Pascal Pasquini, Christoph Wiegreffe, Eleni Zimmer, Medhanie A. Mulaw, Cécile Julier, et al.1:800), GCG (Sigma, G2654, 1:500), C-pep (Cell Signaling, 4593, 1:100), and INS-Alexa647 (Cell Signaling, 3014, 1:80). Next, the samples were washed twice and incubated with Alexa Fluor secondary antibodies (Thermo,

(20) Distinct Fgf21 Expression Patterns in Various Tissues in Response to Different Dietary Regimens Using a Reporter Mouse ModelNutrientsMarch 28, 2025Xinhui Zhang, Zixuan Li, Shuying Wang, Yan ChenInvitrogen, Waltham, MA, USA). Specific antibodies were used to determine co-localization with insulin (3014S, CST), epithelial cadherin (14472T, CST), or glutamine synthetase (11037-2-AP, Proteintech, Rosemont,

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Insulin (C27C9) Rabbit mAb

Insulin (C27C9) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Insulin (C27C9) Rabbit mAb from Cell Signaling Technology

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