Fig 2: Images from immunohistochemical experiments aimed to detect 5-HT1B (top), 5-HT2B (middle) and 5-HT7 (bottom) receptor in the isolated superior mesenteric vein. Images are representative of a minimum of four (4) different animals; arrows point to regions of interest. The left column depicts images of sections incubated with the specific 5-HT primary antibody, and the right column sequential sections incubated without the primary antibody. P, pancreatic tissue; E, endothelium; M, media. Brain sections were used as a positive control for 5-HT7 receptor, and these images are inset into the bottom row with experimental images.

Fig 3: (A). Effect of 5-HT2B (LY272015) and 5-HT7 (SB269970 and LY215840) receptor antagonists on 5-CT relaxation stimulated in the ET-1 (1 nmol/L)-contracted isolated superior mesenteric vein. (B). Ability of 5-CT to lower blood pressure of the conscious rat. Data are graphed as a change from baseline mean arterial blood pressure (MABP) where vehicle MABP = 98.4 ± 2.2 mm Hg, 5-CT MABP = 99.4 ± 2.4 mmHg. Points represent means ± SEM for number of animals indicated in parentheses. *Signify significant differences from vehicle, †from baseline values.