Fig 1: The gene expression levels of HIF2α (A), BNIP3L (B), LC3-II (C), Beclin1 (D), and caspase-3 (E) in the cerebral cortexes, hippocampi, thalami, medulla oblongatae, and cerebella of high- and low-altitude yaks. The gene expression levels represent the mRNA levels in relation to the mRNA expression of the control gene (β-actin). The data are expressed as the means ± SE of 2−ΔΔCt. Bars with different superscripts are significantly different (p < 0.05).
Fig 2: IHC and IF staining of BNIP3L in the cerebral cortexes, hippocampi, thalami, medulla oblongatae, and cerebella of high- and low-altitude yaks. NeuN-positive particles showed green fluorescence under the microscope, while BNIP3L-positive particles showed red fluorescence. DAPI showed blue fluorescence.
Fig 3: (A) The Western blot results. (B–F) The relative expression levels of HIF2α (B), BNIP3L (C), LC3-II (D), Beclin1 (E), and cleaved caspase-3 (F) in the cerebral cortexes, hippocampi, thalami, medulla oblongatae, and cerebella of high- and low-altitude yaks. The values indicate the mean ± SE. Bars with different superscripts are significantly different (p < 0.05).
Fig 4: The IHC optical density analysis values of HIF2α (A), BNIP3L (B), LC3-II (C), Beclin1 (D), and cleaved caspase-3 (E) in the cerebral cortexes, hippocampi, thalami, medulla oblongatae, and cerebella of high- and low-altitude yaks. The data are expressed as the mean ± SE. Bars with different superscripts are significantly different (p < 0.05).
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