Fig 1: PCA inhibited the TLR4/MyD88/NF‐κB pathway in the muscle tissues of CKD rats. (A) The heatmap analysis of important genes in the TLR4/MyD88/NF‐κB signalling pathway. (B–F) Expression levels of Tlr4 (B), Myd88 (C), Ikbkb (D), Nfkbia (E) and Rela (F) in muscles of rats (mean ± SD, n = 6). (G) Western blot analysis of the representative bands of TLR4, MyD88, p‐p65, p65 and GAPDH proteins. (H–K) Statistical results of Western blot expression of TLR4, MyD88, p‐p65 and p65 proteins, with GAPDH as a control (mean ± SD, n = 3–4). (L, M) The levels of Murf1 and Mafbx mRNA expression in rat muscle (mean ± SD, n = 6). (N, O) Representative Western blot bands of MurF1, MAFbx and GAPDH protein. (P, Q) Statistical results of Western blot expression of MurF1 and MAFbx proteins, with GAPDH as a control (mean ± SD, n = 4). *p < 0.05, **p < 0.01, vs. the sham group; # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001, vs. the model group; ns, no significant differences.
Fig 2: PCA inhibited the TLR4/MyD88/NF‐κB signalling pathway in C2C12 myoblasts. The MTT results showed that 40 μM PCA emerged as the most effective concentration. (A) Viability of C2C12 myoblasts after 24‐h exposure to PCA (mean ± SD, n = 5). (B) Viability of C2C12 myoblasts after 24‐h exposure to LPS (mean ± SD, n = 5). (C) LPS‐treated C2C12 myoblasts viability after treatment with PCA for 24 h (mean ± SD, n = 4). (D) Viability of C2C12 myoblasts after 24‐h exposure to PA (mean ± SD, n = 5). (E) Western blot analysis of the representative bands of TLR4, MyD88, p‐p65, p65, MAFbx, MurF1 and GAPDH proteins. (F–H) Statistical results of Western blot expression of TLR4, MyD88, p‐p65 and p65 proteins, with GAPDH as a control (mean ± SD, n = 5). (I, J) Statistical results of Western blot expression of MAFbx and MurF1 proteins, with GAPDH as a control (mean ± SD, n = 4). *p < 0.05, **p < 0.01, ****p < 0.0001, vs. the control group; # p < 0.05, ## p < 0.01, vs. the LPS group; &p < 0.05, &&p < 0.01, vs. the LPS + PCA group.
Supplier Page from Abcam for Anti-Fbx32 antibody