Fig 1: MRW promoted the secretion of 5-HT and activated its receptor pathway in the STC rats. (A) Immunohistochemical results of 5-HT in colon tissue. IOD: integral optical density. (B) ELISA results of 5-HT and TPH in colon tissue. (C) HPLC results of 5-HIAA in colon tissue. (D) Immunofluorescence results of 5-HT4R and CFTR in colon tissue. IOD: integral optical density. (E) WB results of 5-HT4R, CFTR, cAMP, and PKA in colon tissue. All data were normally distributed and analyzed by one-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. model group; & p < 0.05, && p < 0.01, &&& p < 0.001 vs. L-MRW group.
Fig 2: Results of FC sorting of 10,000 or more spermatozoa of five fertile donors after incubation in either capacitating (CM) or non-capacitating (NC) culture medium and in the presence or absence of the CFTR-ion channel inhibitor CFTRinh-172 (24 µM).
Fig 3: Immunofluorescent staining of the CFTR membrane channel in three heterozygous carriers of the Phe508del-pathogenic variant of the CFTR-gene and three healthy controls demonstrated by similar percentages of spermatozoa with the CFTR protein in the membrane, both in seminal sperm (A) and in spermatozoa after swim-up or density gradient centrifugation (B). Representative images of spermatozoa stained with the fluorescent anti-CFTR antibody and with the Hoechst 33342-dye (C).
Fig 4: Suggested model in partially differentiated enterocytes of interactions of NHE3, DRA, and CFTR under baseline conditions and after cAMP. Horizontal lines connecting NHE3, DRA, and CFTR above microvilli indicate the extent of physical interaction (the more the horizontal lines, the greater the physical interaction). Vertical lines in microvilli associated with each protein indicate the percent of the total protein in the apical membrane (thick line high percentage).
Fig 5: (A). Differentiation-dependent protein expression of NHE3, DRA, and CFTR in human enteroids. n = 3 (paired t-tests). Similar time-dependent mRNA (B) and protein (C) expression of NHE3, DRA, and CFTR in Caco-2-BBe monolayers with days post confluency.
Supplier Page from Abcam for Anti-CFTR antibody