Fig 1: Silencing SOS2 reverses the tumor-promoting effects of miR-148a inhibitors in A549R cells. (A) Cell survival curve was generated in A549 cells transfected with NC inhibitors, miR-148a inhibitors, si-SOS2 and miR-148a inhibitors + si-SOS2 at different doses of X-ray radiation. (B-D) CCK-8, Transwell and Matrigel assays were performed to analyze the (B) cell proliferation, (C) migration and (D) invasion capacity in A549R cells after transfection with NC inhibitors, miR-148a inhibitors, si-NC, si-SOS2 and miR-148a inhibitors + si-SOS2. **P<0.01 and ***P<0.001. miR, microRNA; si-, small interfering; NC, negative control.
Fig 2: miR‐148a‐3p directly suppresses the expression of SOS2. A, Venn diagram showed overlapped target genes of miR‐148a‐3p in three databases. B, Relative luciferase activity in Ishikawa and End1/E6E7 cells transfected with miR‐NC or miR‐mimics and SOS2‐wild‐type (wt) or SOS2‐mutation (mut), respectively. C, The protein expression of SOS2 in E2‐treated Ishikawa and End1/E6E7 cells was tested by Western blot. D, Cell migration of E2‐treated or untreated Ishikawa and End1/E6E7 cells after NC, si‐SOS2 and/or miR inhibitor transfection was analysed by transwell assay, respectively. E, Protein expression of E‐cadherin and N‐cadherin in E2‐treated or E2‐untreated Ishikawa and End1/E6E7 cells after NC, si‐SOS2 and/or miR inhibitor transfection was analysed by Western blot. Results show as mean ± SD. **Means compared with control group, P < 0.05. ##Means compared with E2‐treated group, P < 0.05
Fig 3: SOS2 is a direct target of miR-148a. (A) Potential associating sites of SOS2 in the 3′-UTR region with miR-148a. (B and C) Reverse transcription-quantitative PCR and western blot analysis was used to assess SOS2 expression after transfection with miR-148a mimics or miR-148a inhibitors. (D) Correlation analysis of miR-148a and SOS2 expression levels in patients with non-small cell lung cancer. (E and F) Dual-luciferase reporter gene analysis of miR-148a and SOS2 in (E) A549R and (F) H358R cells. **P<0.01 and ***P<0.001. miR, microRNA; UTR, untranslated region; NC, negative control; WT, wild-type; MUT, mutant; ns, not significant.
Supplier Page from Abcam for Anti-SOS2 antibody