Fig 1: AChR-clustering regulator rapsyn interacts with drebrin. (A) Coimmunoprecipitation of overexpressed wild-type drebrin (DbnE-WT-GFP) with rapsyn (Rapsyn-mCherry) in HEK293 cells. (B) Coimmunoprecipitation of overexpressed phospho-dead drebrin mutant at Ser142 (DbnE-142A-GFP) with rapsyn (Rapsyn-mCherry) in HEK293 cells. (C) Coimmunoprecipitation of overexpressed EB3-GFP with rapsyn (Rapsyn-mCherry) in HEK293 cells. Elute—eluates from the beads incubated with equal volumes of lysates, Flow-through (FT)—lysates incubated with the beads.
Fig 2: Proposed model of drebrin role in the regulation of postsynaptic machinery clustering. Rapsyn anchors AChRs to actin and microtubules (- Drebrin). Drebrin bundles actin filaments and acts as a cross-linker between actin cytoskeleton and microtubule network under AChR clusters. Drebrin interacts with rapsyn, a cluster-stabilizing protein, and EB3, a microtubule plus-end protein, and recruits microtubules under AChR clusters (+ Drebrin). Illustration created with BioRender.com (www.biorender.com, last access 26 August 2021).
Fig 3: mRNA expression of NMJ‐related factors in the late recovery phase from an acute bout of muscle contraction. (a) Agrn, (b) Lrp4, (c) Musk, (d) Dok7, (e) Chrna1, (f) Chrnb1, (g) Chrnd, (h) Rapsn and (i) Chrne. Mean values are shown with individual values in bar and dot plots. *P < 0.05 versus CON. AU, arbitrary units; CON, control.
Fig 4: mRNA expression of NMJ‐related factors in the early recovery phase from an acute bout of muscle contraction. (a) Agrn, (b) Lrp4, (c) Musk, (d) Dok7, (e) Chrna1, (f) Chrnb1, (g) Chrnd, (h) Rapsn and (i) Chrne. Mean values are shown with individual values in bar and dot plots. *P < 0.05 versus CON. AU, arbitrary units; CON, control.
Supplier Page from Abcam for Anti-Rapsyn antibody [EPR9759]