PIP4K2A (D83C1) Rabbit mAb 5527S from Cell Signaling Technology

Product Specs
ItemPIP4K2A (D83C1) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number5527S
Quantity100 µl
ApplicationsW
ReactivityH, M, R, Mk, B, Pg
ClonalityMonoclonal
TargetPIP4K2A
HostRabbit
NCBI Full Gene Namephosphatidylinositol-5-phosphate 4-kinase type 2 alpha
NCBI Gene AliasesPI5P4KA, PIP5K2A, PIP5KII-alpha, PIP5KIIA, PIPK
Research AreaCa, cAMP and Lipid Signaling
IsotypeIgG
CloneD83C1
Molecular Weight50

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Cell Signaling Technology

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Danvers, Massachusetts 01923

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Phone: 877-616-CELL
Phone: 978-867-2388
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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Regulation of the MDM2-p53 nexus by a nuclear phosphoinositide and small heat shock protein complexJournal of Biological ChemistrySeptember 1, 2025Jeong Hyo Lee, Mo Chen, Tianmu Wen, Richard A. Anderson, Vincent L. Cryns#SC-13132, Santa Cruz Biotechnology), αBC (D6S9E, #45844, Cell Signaling), PIPKIIα (PIP4K2A, clone D83C1, #5527, Cell Signaling), GAPDH (clone 0411, #SC-47724, Santa Cruz Biotechnology), and polyclonal antibodies

(2) Regulation of NRF2 by Stably associated Phosphoinositides and Small Heat Shock Proteins in Response to StressJournal of Biological ChemistryJune 12, 2025Changliang Chen, Noah D. Carrillo, Mo Chen, Tianmu Wen, Poorwa Awasthi, Richard A. Anderson, Vincent L. Cryns(#12165), HSP90 (#4877), Keap1 (#8047), HSP27 (D6W5V) (#95357), PIPKIα (#9693), PIPKIγ (#3296), PIPKIIα (#5527), PIPKIIβ (#9694), and DYKDDDDK Tag (#14793) were purchased from Cell Signaling Technology; HSP27 (ADI-SPA-801)

(3) PARP inhibitors accumulate B7-H3 on fibroblasts via blocking autophagic flux to potentiate immune evasion in ovarian cancerOncoimmunologyJune 11, 2025Tian Fang, Yu Xia, Ying Li, Sen Xu, Huayi Li, Siyuan Wang, Pu Huang, Yiyu Qian, Ping Jin, Ning Jin, et al.18420–1-AP, 1:1000), anti-LC3B (Proteintech 14600–1-AP, 1:1000), anti-PI5P4Kα (Cell signaling technology, 5527S, 1:1000) and anti-GAPDH overnight at 4℃. Secondary antibodies were obtained from Servicebio, and visualization

(4) Noncanonical PI(4,5)P2 coordinates lysosome positioning through cholesterol traffickingbioRxivJanuary 3, 2025Ryan M. Loughran, Gurpreet K. Arora, Jiachen Sun, Alicia Llorente, Sophia Crabtree, Kyanh Ly, Ren-Li Huynh, Wonhwa Cho, Brooke M. Emerlingprobed overnight at 4°C with the appropriate primary antibody. Antibodies used were as follows: PIP4K2α (5527; Cell Signaling), PIP4K2β (9694; Cell Signaling), P-p70S6K (9234; Cell Signaling), p70S6K (9202; Cell Signaling

(5) Exploring the Potential Role of Oligodendrocyte-Associated PIP4K2A in Alzheimer’s Disease Complicated with Type 2 Diabetes Mellitus via Multi-Omic AnalysisInternational Journal of Molecular SciencesJune 17, 2024Doan Phuong Quy Nguyen, Amadou Wurry Jallow, Yi-Fang Lin, Yung-Feng Linincubated with a monoclonal primary antibody specifically against PIP4K2A (Cell Signaling Technology, #5527, Danvers, MA, USA) at 4 °C overnight. After thorough washing, the membrane was incubated with HRP-labeled

(6) ATG9 resides on a unique population of small vesicles in presynaptic nerve terminalsAutophagyApril 1, 2024Binotti B, Ninov M, Cepeda AP, Ganzella M, Matti U, Riedel D, Urlaub H, Sambandan S, Jahn R.Synaptic Systems (SYSY). LC3B (ALX-803-081-C100) from ENZO, ATG2A (PD041) from MBL, PIP4K2A (D83C1; 5527) from Cell Signaling Technology. Whole IgG Sheep (Sh-003-Z) from Dianova, and the monoclonal rabbit

(7) PIP4K2B Protein Regulation by NSD1 in HPV-Negative Head and Neck Squamous Cell CarcinomaCancers (Basel)March 17, 2024Iuliia Topchu, Igor Bychkov, Ekaterina Roshchina, Petr Makhov, Yanis Boumber(Cell Signaling Technology, #9692;, Danvers, MA, USA; 1:1000), PIP4K2A (Cell Signaling Technology, #5527; 1:1000), PIP4K2C (Thermo Fisher Scientific, #H00079837-M01A; 1:1000), Di-Methyl-Histone H3 (Lys36)

(8) Pharmacological Inhibition of PIP4K2 Potentiates Venetoclax-Induced Apoptosis in Acute Myeloid LeukemiaInternational journal of molecular sciencesNovember 29, 2023Lima K, Carvalho MFL, Pereira-Martins DA, Nogueira FL, de Miranda LBL, Nascimento MCD, Cavaglieri RC, Schuringa JJ, Machado-Neto JA, Rego EM.G: BOX Chemi XX6 gel document system (Syngene Cambridge, Cambridge, UK). Antibodies directed against PIP4K2A (#5527), PIP4K2B (#9694), PARP1 (#9542), γH2AX (#9718), and α-tubulin (#2144) were obtained from Cell Signaling Technology

(9) Development of potent and selective degraders of PI5P4KγEuropean Journal of Medicinal ChemistryFebruary 5, 2023Wenzhi Ji, Eric S. Wang, Theresa D. Manz, Jie Jiang, Katherine A. Donovan, Xianmixinuer Abulaiti, Eric S. Fischer, Lewis C. Cantley, Tinghu Zhang, Nathanael S. Grayanti-following proteins: β-Actin (Cell Signaling Technology, cat#3700). PI5P4Kα (Cell Signaling Cat# 5527) PI5P4Kβ (Cell Signaling Technologies Cat# 9694), PI5P4Kγ (Sigma-Aldrich Cat# HPA058551); Vinculin(Cell Signaling Technologies

(10) PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplayDevelopmental cellJune 7, 2021Ravi A, Palamiuc L, Loughran RM, Triscott J, Arora GK, Kumar A, Tieu V, Pauli C, Reist M, Lew RJ, et al.4°C with the appropriate primary antibody. Antibodies used were as follows: PIP4K2α (Cell Signaling; 5527), PIP4K2β (Cell Signaling; 9694), β-actin (Santa Cruz Biotechnology; sc-517582) and a-tubulin (Sigma-Aldrich;

(11) Conserved RNA Binding Activity of Phosphatidyl Inositol 5-Phosphate 4-Kinase (PIP4K2A)Frontiers in molecular biosciencesJanuary 1, 2021Behari J, Borkar P, Vindu A, Dandewad V, Upadrasta S, Shanmugam D, Seshadri V.and PIP4K2A pull-down were loaded on 15% SDS PAGE along with input and probed with anti-PIP4K2A (CST#5527) and anti-eIF4EBP1 (CST #9644) antibodies. Luciferase Assay PIP4K2A, along with firefly luciferase

(12) Targeting the PI5P4K Lipid Kinase Family in Cancer Using Covalent InhibitorsCell chemical biologyMay 21, 2020Sivakumaren SC, Shim H, Zhang T, Ferguson FM, Lundquist MR, Browne CM, Seo HS, Paddock MN, Manz TD, Jiang B, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies Rabbit monoclonal anti PI5P4Kα,Cell Signaling,"Cat# 5527, RRID:AB_2722636" Rabbit monoclonal anti PI5P4Kβ,Cell Signaling Technologies,"Cat# 9694, RRID:AB_2164572" Rabbit

(13) Discovery and Structure-Activity Relationship Study of (Z)-5-Methylenethiazolidin-4-one Derivatives as Potent and Selective Pan-phosphatidylinositol 5-Phosphate 4-Kinase InhibitorsJournal of medicinal chemistryMay 14, 2020Manz TD, Sivakumaren SC, Ferguson FM, Zhang T, Yasgar A, Seo HS, Ficarro SB, Card JD, Shim H, Miduturu CV, et al.and Reagents. Antibodies purchased from Cell Signaling Technology (Hanover, MA) were anti-PI5P4Kα (5527) and anti-PI5P4Kβ (9694). Anti-GAPDH (MAB374) was obtained from EMD Millipore Corporation (Merck KGaA,

(14) PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent MechanismCell reportsMay 14, 2019Wang DG, Paddock MN, Lundquist MR, Sun JY, Mashadova O, Amadiume S, Bumpus TW, Hodakoski C, Hopkins BD, Fine M, et al.REAGENT or RESOURCE,SOURCE,IDENTIFIER Antibodies,, ,, PIP4K2A,Cell Signaling Technologies,Cat# 5527; RRID:AB_2722636 PIP4K2B,Cell Signaling Technologies,Cat# 9694; RRID:AB_2164572 PIP4K2C,Proteintech,Cat#

(15) WNT Activates the AAK1 Kinase to Promote Clathrin-Mediated Endocytosis of LRP6 and Establish a Negative Feedback LoopCell reportsJanuary 2, 2019Agajanian MJ, Walker MP, Axtman AD, Ruela-de-Sousa RR, Serafin DS, Rabinowitz AD, Graham DM, Ryan MB, Tamir T, Nakamichi Y, et al.– Clathrin (4796), LRP6 (3395), phospho-LRP6 (S1490) (2568), phospho-AP2M1 (T156) (7399S), PIP4K2A (5527), phospho-CTNNB1 (S33/37/T41) (9561S); Sigma-Aldrich – FLAG M2 (F3165), HA (11867423001), and beta-tubulin

(16) Phosphatidylinositol-5-Phosphate 4-Kinases Regulate Cellular Lipid Metabolism By Facilitating AutophagyMolecular cellMay 3, 2018Lundquist MR, Goncalves MD, Loughran RM, Possik E, Vijayaraghavan T, Yang A, Pauli C, Ravi A, Verma A, Yang Z, et al.probed overnight at 4°C with the appropriate primary antibody. Antibodies used were as follows: PIP4K2α (5527; Cell Signaling), PIP4K2β (9694; Cell Signaling), P-p70S6K (9234; Cell Signaling), p70S6K (9202; Cell Signaling

(17) The downstream of tyrosine kinase 7 is reduced in lung cancer and is associated with poor survival of patients with lung cancerOncology ReportsMay 1, 2017Gang Chen, Hefen Yu, Lucy Satherley, Catherine Zabkiewicz, Jeyna Resaul, Huishan Zhao, Hu Mu, Xiuyi Zhi, Junqi He, Lin Ye, et al.Yuan ZM: PRR14 is a novel activator of the PI3K pathway promoting lung carcinogenesis. Oncogene 35: 5527-5538, 2016. 4. Lemmon MASJ and Schlessinger J: Cell signaling by receptor tyrosine kinases. Cell 141:

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PIP4K2A (D83C1) Rabbit mAb

PIP4K2A (D83C1) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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PIP4K2A (D83C1) Rabbit mAb from Cell Signaling Technology

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