Fig 1: APC/C subunit requirements for Mcl‐1 degradation during mitotic arrest (related to Fig 3) A, BConfirmation of siRNA knockdown of APC3, APC8 and APC11 in HeLa cells stably expressing (A) YFP‐Mcl‐1 or YFP‐Mcl‐1ΔIR or (B) cyclin A‐Venus or cyclin B1‐Venus.C, DAPC3, APC8 or APC11 were knocked down by siRNA prior to treatment with nocodazole to induce mitotic arrest. The effect on the degradation of cyclin A‐Venus protein in 830 nM nocodazole (C) and cyclin B1‐Venus protein in 33 nM nocodazole (D) was analysed by time‐lapse microscopy. The nocodazole concentration was reduced to analyse cyclin B1 degradation since cyclin B1 was stable for the duration of the experiment at a higher nocodazole concentration (830 nM, data not shown). Representative images (left) and quantification (right) are shown. Error bars represent ±SD, n = 3. Scale bar, 10 μm.EConfirmation of siRNA knockdown of APC10 in HeLa cells stably expressing YFP‐Mcl‐1 WT or ΔIR, or cyclin B1‐Venus.FAPC10 was knocked down by siRNA prior to treatment with nocodazole (33 nM) to induce mitotic arrest. The effect on the degradation of cyclin B1‐Venus was analysed by time‐lapse microscopy. Error bars represent ±SD, n = 3. Source data are available online for this figure.