BNIP3 Antibody AF4147 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemBNIP3 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberAF4147
NCBI Gene AliasesNIP3, HABON
ApplicationsImmunohistochemistry,Western Blot
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman
Conjugate/TagUnconjugated
Quantity100 ug (also 25 ug)
HostGoat
IsotypeIgG
TargetBNIP3
NCBI Full Gene NameBCL2 interacting protein 3
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human BNIP3, Ser2-Glu160, Accession # Q12983
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human BNIP3 in Western blots. In Western blots, this antibody shows no cross-reactivity with recombinant human BNIP3L.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Iron content of glioblastoma tumours and role of ferrous iron in the hypoxic response in vitroFront OncolMarch 7, 2025Citra Praditi, Eira Beverley-Stone, Malcolm Reid, Eleanor R. Burgess, Rebekah L. Crake, Margreet C.M. Vissers, Janice A. Royds, Tania L. Slatter, Gabi U. Dachs, Elisabeth Phillips(HIF-1α (1:250, 610959, BD Transduction Laboratories, USA), Bcl2-interacting protein 3 (BNIP3, 1:1000, AF4147, R&D Systems, Invitro Technologies, Auckland, NZ), carbonic anhydrase 9 (CA-IX, 1:1000, AF2188, R&D Systems

(2) Role of Sodium‐Dependent Vitamin C Transporter‐2 and Ascorbate in Regulating the Hypoxic Pathway in Cultured Glioblastoma CellsJournal of Cellular BiochemistryOctober 9, 2024Eleanor R. Burgess, Citra Praditi, Elisabeth Phillips, Margreet C. M. Vissers, Bridget A. Robinson, Gabi U. Dachs, George A. R. Wigginsblocking solution, against HIF‐1α (1:250, BD Bioscience, Cat No. 610959), BNIP3 (1:1000, R&D systems AF4147) and β‐actin (1:1000, Sigma‐Aldrich, A5441), then with relevant secondary antibodies followed by detection

(3) Increased Ascorbate Content of Glioblastoma Is Associated With a Suppressed Hypoxic Response and Improved Patient SurvivalFrontiers in oncologyJanuary 1, 2022Burgess ER, Crake RLI, Phillips E, Morrin HR, Royds JA, Slatter TL, Wiggins GAR, Vissers MCM, Robinson BA, Dachs GU.R&D Systems AF2188), BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3, 1:1000, R&D Systems AF4147), phosphoglycerate kinase 1 (PGK1, 1:5000, Abcam Ab38007) and glucose transporter 1 (GLUT1, 1:1000,

(4) Ascorbate modulates the hypoxic pathway by increasing intracellular activity of the HIF hydroxylases in renal cell carcinoma cellsHypoxia (Auckland, N.Z.)January 1, 2019Wohlrab C, Kuiper C, Vissers MC, Phillips E, Robinson BA, Dachs GU.USA, 3434), HIF-2α (1/400, R&D Systems, Minneapolis, MN, USA, AF2997), BNIP3 (1/1000, R&D Systems, AF4147), cyclin D1 (1/10,000, Abcam, Cambridge, UK, ab134175), GLUT1 (1/1000; Abcam, ab32551) or ß-actin (1/10,000,

(5) The Association Between Ascorbate and the Hypoxia-Inducible Factors in Human Renal Cell Carcinoma Requires a Functional Von Hippel-Lindau ProteinFrontiers in oncologyJanuary 1, 2018Wohlrab C, Vissers MCM, Phillips E, Morrin H, Robinson BA, Dachs GU.(1/800, BD Biosciences, BD610958), HIF-2α (1/400, R&D Systems, AF2997), BNIP3 (1/1000, R&D Systems, AF4147), CA9 (1/200, R&D Systems, AF2188), cyclin D1 (1/10,000, Abcam, ab134175), GLUT1 (1/1000; Abcam, ab32551)

(6) Expression and Functional Characterization of the BNIP3 Protein in Renal Cell CarcinomasTranslational oncologyDecember 1, 2017Macher-Goeppinger S, Keith M, Hatiboglu G, Hohenfellner M, Schirmacher P, Roth W, Tagscherer KE.anti-actin (Sigma Aldrich, Munich, Germany; A5441) and anti-BNIP3 (R&D Systems, Wiesbaden, Germany, AF4147). CoCl2 (60818), etoposide (E1383), and staurosporine (4400) were obtained from Sigma Aldrich. The RCC

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Human BNIP3 Antibody

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BNIP3 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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BNIP3 Antibody from R&D Systems, a Bio-Techne Brand

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