Fig 1: Validation of miRNA and cross-gene expression. (a) Representative immunohistochemical results of CALML4, SMAD6, PITX2, and TGFB2. (b) IHC staining positive rate of CALML4, SMAD6, PITX2, and TGFB2. (c) The expression of hsa-miR-196b-3p and hsa-miR-196b-5p from TCGA database. (d) The expression of hsa-miR-196b-3p and hsa-miR-196b-5p in 69 patients with or without H. pylori infection, and the expression level of (e) hsa-miR-196-3p, not (f) hsa-miR-196-3p, in patients with H. pylori infection was significantly higher. All tests were independently manipulated at least 3 times, with the conversion of gene expression value to log2 (fold change).
Fig 2: Inter-donor heterogeneity for fold increase in TGFB2 gene induction versus corresponding non-induced hMSC control cells derived from donors #1–#9. (A) Gene upregulation in response to osteogenic medium treatment for one week (1 W) (black bars) or two weeks (2 W) (grey bars). Error bars indicate the pooled coefficient of variation. (B) The fold-induction ratio for values at one or two weeks post induction. Interpolation used the GUSB/YWAHZ reference gene pair. Independent t-test between hBM-MSC and hAT-MSC groups * p < 0.05.
Fig 3: Prognostic value of hub genes for GC patients from TCGA. Overall survival time of GC patients with over- or underexpression of (a) CALML4, (b) PITX2, (c) hsa-miR-196b-3p, (d) SMAD6, (e) TGFB2, and (f) hsa-miR-196b-5p. CALML4: calmodulin-like protein 4; PITX2: paired-like homeodomain 2; SMAD6: SMAD family member 6; TGFB2: transforming growth factor beta 2.
Fig 4: Prospective identification of osteogenic potency derived from (A) the inter-donor heterogeneity for fold-induction of gene expression for DCN and TGFB2 after one week of osteoinduction, tracing an inverse correlation expression pattern across the hMSC derived from donors #1 to #9, allowing (B) a correlative plot between these two genes discriminating non-bone-forming hMSC (red zone) versus bone-forming hMSC (green zone). (C) Dendrogram of Euclidian cluster analysis for all donors using gene induction data from DCN and TGFB2.
Supplier Page from Abcam for Anti-TGF beta 2 antibody