AIF-1/Iba1 Antibody NB100-2833 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemAIF-1/Iba1 Antibody
CompanyNovus Biologicals, a Bio-Techne Brand
Price $419.00Supplier Page View Company Product Page
Catalog NumberNB100-2833
Concentration0.5 mg/ml
Conjugate/TagUnconjugated
ApplicationsIF/IHC,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Peptide ELISA,Immunocytochemistry/ Immunofluorescence
TargetAIF-1/Iba1
HostGoat
Quantity0.1 mg
ReactivityHuman,Mouse
FormatTris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
ImmunogenThis AIF-1/Iba1 Antibody was developed against a peptide with sequence C-NKQFLDDPKYSSDED corresponding to internal region according to NP_001614.3
NCBI Full Gene Nameallograft inflammatory factor 1
NCBI Gene AliasesIRT1, IBA1, AIF-1, IRT-1
ClonalityPolyclonal
PurityImmunogen affinity purified
Research AreaAutoimmune Diseases, Cancer, Cell Biology, Cell Cycle and Replication, Immunology, Inflammation, Lipid and Metabolism, Microglia Markers, Neuroscience
IsotypeIgG
Molecular Weight16.7 kDa
Antibody TypePrimary Antibodies
StorageStore at -20C. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: This AIF-1/Iba1 Antibody is expected to recognize isoform 3 (NP_001614.3) only.

Sizes Available: 0.1 mg

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Questions and Answers

Can this antibody be used to study microglial activation in mouse models?

Yes, the AIF-1/Iba1 Antibody has been utilized in studies to assess microglial activation in mouse models. For instance, it was employed in a study investigating the effects of prenatal exposure to lipopolysaccharides on neuroinflammation, where it was used at a dilution of 1:500 for immunohistochemistry to visualize microglial cells ().

Is this antibody effective for detecting microglial activation in the context of neurodegenerative diseases?

The AIF-1/Iba1 Antibody has been shown to be effective in detecting microglial activation associated with neurodegenerative diseases. In research focused on Alzheimer's disease, it was used to evaluate microglial activation in mouse brain tissue, demonstrating its utility in studies related to neuroinflammation and cognitive deficits ().

What applications has this antibody been validated for in scientific research?

The AIF-1/Iba1 Antibody has been validated for several applications, including Western Blotting, ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry. Its versatility makes it suitable for various experimental setups in neuroscience research.

What species can this antibody be used with, and is it suitable for human studies?

This antibody is reactive with both mouse and human tissues, making it suitable for studies involving both species. Its application in human studies is supported by its use in research that examines microglial function and neuroinflammation in human-related contexts.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Host–virus determinants of Ebola virus persistence in a human cerebral organoid modelNature MicrobiologyJune 12, 2026Lina Widerspick, Santiago Vidal Freire, Johanna F. Steffen, Shruti Shirsathe, Petra Allartz, Molly A. Vickers, Christoph Henkel, Pedro Neira Pelén, Julia Nave, Monika Rottstegge, et al.BSA (v/v) in PBS. Specific antibodies and dilutions included: AIF1 antibody, goat, polyclonal (Novus, NB100-2833; 1:100–1:200), rabbit anti-GFAP (Zytomed, RBK037; 1:200), rabbit CD68 (D4B9C) XP (Cell Signaling Technology,

(2) Human organoids for Risk Group 4 virus research: a new frontier in investigating Nipah virus infection of the central nervous systemJournal of VirologyNovember 25, 2025Gabriella Worwa, Shuǐqìng Yú, Amanda M. W. Hischak, Julie P. Tran, Jeremy J. Bearss, John Bernbaum, Daniel B. Woodburn, Bapi Pahar, Jillian Geiger, Louis M. Huzella, et al.#NBP2-72838AF750, Novus Biologicals), and anti-NiV antibody (in-house). The AIF-1/Iba1 Antibody, goat, polyclonal (#NB100-2833, Novus Biologicals, Centennial, CO, USA) was used in an in-house custom conjugation process utilizing

(3) Neonatal Subventricular Zone Neural Stem Cells Release Extracellular Vesicles that Act as a Microglial MorphogenSSRN Electronic JournalMarch 31, 2022Mary C. Morton, Victoria N. Neckles, Caitlin Seluzicki, David M. Feliciano2011). Antibodies used included Nestin (Novus Biologicals, NB100-1604), CD9 (eBioscience, 14-0091-81), Iba1 (Novus, NB100-2833), CD68 (Bio-Rad, MCA1957), CD11b (Bio-Rad, MCA711G), or GFAP (Cell Signaling Technology, 12389S). Secondary

(4) Loss of Metabotropic Glutamate Receptor 5 Function on Peripheral Benzodiazepine Receptor in Mice Prenatally Exposed to LPSPloS oneJanuary 1, 2015Arsenault D, Coulombe K, Zhu A, Gong C, Kil KE, Choi JK, Poutiainen P, Brownell AL.antibodies were used: goat polyclonal anti-Iba1 (ionized calcium binding adaptor molecule-1, cat. number: NB100-2833, 1:500, Novus Biological), mouse monoclonal anti-GFAP (glial fibrillary acidic protein, cat. number:

(5) Neonatal Subventricular Zone Neural Stem Cells Release Extracellular Vesicles that Act as a Microglial MorphogenCell reportsJanuary 1, 0001"Morton MC, Neckles VN, Seluzicki CM, Holmberg JC, Feliciano DM."

(6) A transgenic inducible GFP extracellular-vesicle reporter (TIGER) mouse illuminates neonatal cortical astrocytes as a source of immunomodulatory extracellular vesiclesScientific reportsJanuary 1, 0001"Neckles VN, Morton MC, Holmberg JC, Sokolov AM, Nottoli T, Liu D, Feliciano DM."being mounted. Antibodies used were GFAP (Cell Signaling: Catalog #12389), IBA1 (Novus Bio: Catalog #NB100-2833), CD11b (Bio-Rad: Catalog #MCA711G), and Glutamine Synthetase (Sigma Aldrich: Catalog #G2781), Rab27a

(7) HBO treatment enhances motor function and modulates pain development after sciatic nerve injury via protection the mitochondrial functionJournal of translational medicineJanuary 1, 0001"Awad-Igbaria Y, Ferreira N, Keadan A, Sakas R, Edelman D, Shamir A, Francous-Soustiel J, Palzur E."(1:250, AP31284PU-N, ORIGENE), anti-cleaved caspase 3 (1:500, TA336323, ORIGENE), anti-Iba1 (1:500, NB100-2833, NOVUS), anti-MMP-9 (1:500, 10,375–2-AP, Proteintech). After the first immunoreaction, the slides were

(8) IL-12p40 promotes secondary damage and functional impairment after spinal cord contusional injuryJournal of neuroscience researchJanuary 1, 0001"Rosas Almanza J, Stehlik KE, Page JJ, Xiong SH, Tabor EG, Aperi B, Patel K, Kodali R, Kurpad S, Budde MD, et al."

(9) Lifelong choline supplementation ameliorates Alzheimer's disease pathology and associated cognitive deficits by attenuating microglia activationAging cellJanuary 1, 0001"Velazquez R, Ferreira E, Knowles S, Fux C, Rodin A, Winslow W, Oddo S."dilution 1:200), and 6E10 (Catalog MAB1560, dilution 1:3000); Novus Biologicals: Goat‐Iba1 (Catalog #NB100‐2833, dilution 1:200); and Wako: Mouse‐Iba1 (Catalog NCNP24, dilution 1:200). 4.6 ELISA To asses soluble

(10) Astrocyte-microglia crosstalk in the hippocampus mediates cognitive impairments induced by chronic intermittent hypoxiaNeurobiology of diseaseJanuary 1, 0001"Wu ZH, Zhai MR, Wang YR, Ren L, Pan J, Xiao L, Liu YH."

(11) A Small Compound Targeting Prohibitin with Potential Interest for Cognitive Deficit Rescue in Aging mice and Tau Pathology TreatmentScientific reportsJanuary 1, 0001"Guyot AC, Leuxe C, Disdier C, Oumata N, Costa N, Roux GL, Fernandez-Varela P, Duchon A, Charbonnier JB, Herault Y, et al."Abcam #ab105389), SOX-2 (1:500; Abcam #ab171380), GFAP (1:500; Abcam #ab7260), Iba-1 (1:500; Novus #NB100–2833), prohibitin (1:500; Novus #NBP2–67334) and anti-rabbit Alexa Fluor 477 (1:250), anti-rabbit Alexa Fluor

(12) "Chronic oral application of a periodontal pathogen results in brain inflammation, neurodegeneration and amyloid beta production in wild type mice"PloS oneJanuary 1, 0001"Ilievski V, Zuchowska PK, Green SJ, Toth PT, Ragozzino ME, Le K, Aljewari HW, O'Brien-Simpson NM, Reynolds EC, Watanabe K."

(13) Long-term selective stimulation of transplanted neural stem/progenitor cells for spinal cord injury improves locomotor functionCell reportsJanuary 1, 0001"Kawai M, Imaizumi K, Ishikawa M, Shibata S, Shinozaki M, Shibata T, Hashimoto S, Kitagawa T, Ago K, Kajikawa K, et al."

(14) Allograft inflammatory factor-1 (AIF-1) is crucial for the survival and pro-inflammatory activity of macrophagesInternational immunologyJanuary 1, 0001"Yang ZF, Ho DW, Lau CK, Lam CT, Lum CT, Poon RT, Fan ST."

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