Western blot analysis was performed on whole cell extracts (30 µg lysate) of NCCIT (Lane1), Ntera-2 (Lane2), F9 (Lane 3), HeLa (Lane 4) and Hep G2 (lane 5). The blots were probed with Anti-Oct-4 Mouse Monoclonal Antibody (Product # MA1-104, 1:500-1:2000 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 38 kDa corresponding to Oct-4 was observed across cell lines tested, expect in HeLa and Hep G2. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).