Fig 1: PES-induced loss of viability in PEL cell lines is dose and time dependent. (a) BC3 and BCBL1 were treated for 24 h with different PES concentrations (10, 20 and 30 µM) or (b) for different times (12, 24 and 48 h) with 20 µM of PES. Cells were counted by trypan-blue exclusion and mean±S.D. of three different experiments is reported. (c) Comparison of PES sensitivity between PEL cells (BC3) vs normal human B cells isolated from two different healthy donors. Cells were counted by trypan-blue exclusion and mean±S.D. of three different experiments is reported. (d) BC3, BCBL1 and normal B cell (from two different healthy donors) lysates were used to perform western blot analysis to evaluate the HSP70 protein expression. A ß-actin antibody was used as protein-loading control. The numbers indicate the ratio HSP70/ß-actin