Fig 1: Identification and osteogenic differentiation of BMSCs. (A) Cell morphology of MBD-BMDC and Normal-BMSC. Magnification, ×100. (B) Detection of surface antigens CD34, CD45, CD44, CD90 and CD105 in MBD-BMDC and Normal-BMSC. (C) Alizarin red staining of MBD-BMDC and Normal-BMSC before and after osteogenic differentiation. Magnification, ×100. The mRNA expressions of alkaline phosphatase (ALP) (D), osteopontin (OPN) (E) and osteocalcin (OC) (F) were detected by RT-PCR, and ACTIN was applied as the normalization standard. * P < 0.05, ** P < 0.01 and *** P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)