Fig 2: Representative immunohistochemical staining images of CDK5 and p21 in 2 patients’ ccRCC and adjacent normal tissues. a-d The strong staining of CDK5 in normal tissue and lower staining in cancerous tissues. e, g. f, h. The strong staining of p21 in normal tissue and lower staining in cancerous tissues. Bar,100 um

Fig 3: The overall survival curves based on the CDK5 and p21 expression in ccRCC patients. a-c showed the survival results based on the TMAs, and b-d showed the survival results based on the Oncomine database

Fig 4: Western blotting of extracts from FTL siRNA transfected cell lines indicates suppression of the expression levels of FTL compared with cells transfected with NC siRNA, increased expression levels of p21 and p27 and decreased levels of CDK2 and pRb. The graph shows the relative expression levels of each protein in the FTL siRNA-transfected cell line compared with NC. FTL, ferritin light chain; pRB, phosphorylated retinoblastoma protein; NC, negative control; siRNA, short interfering RNA.

Fig 5: In vivo impact of senescent fibroblasts on cancer cells. (A) Group A (LoVo and LV-GALC fibroblast cells) and Group B (LoVo and LV-NC fibroblast cells) mice, tumors sizes, and green fluorescence staining were shown. Tumor volume (B) and weight data for the two groups of mice. p < 0.05 was considered statistically significant. (C) Group A (LoVo and LV-GALC fibroblast cells) and group B (LoVo and LV-NC fibroblast cells) mice tumors were stained with P16, P21, and P53 using IHC.