Fig 2: Alpha-2-macroglobulin (A2M) was compared among the three groups for validation by western blot (WB) analysis. Upper figure: Three clinical hypocalcemia (CH) group samples and three subclinical hypocalcemia (SH) group samples were subjected to western blotting analysis of A2M including two normal plasma controls. Lower figure: A2M protein expression in all cows.

Fig 3: Pathway analysis. This is an integrated pathway to show the relationship between differentially expressed proteins and Ca regulation. The location and physiological process are indicated in italic and underlined font, respectively. APOA4, apolipoprotein A-IV; GC, vitamin D-binding protein; A2M, A2M protein; CBP, calcium binding protein; F2, blood coagulation factor 2; F10, blood coagulation factor 10; FGA, fibrinogen A chain; FGB, fibrinogen B chain. DCT cell, distal convoluted tubular cell; CNT cell, connecting tubular cell.