CDCA3 Polyclonal Antibody bs-7894R from Bioss Inc.

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Frontiers in Immunology
June 27 2024

Fig 1: Validation of six immune-related hub gene. (A) RNA expression of six hub genes measured by qRT-PCR. (B) Western blot analysis of NUF2 and CDCA3 protein levels in hearts and the quantification of protein bands. Data are mean ± SD for n=8 mice. (C) Double immunofluorescence analysis of CDCA3 (green) and cardiac myocytes (α-actin, red) in heart samples. (D) Double immunofluorescence analysis of NUF2 (green) and cardiac myocytes (α-actin, red) in heart samples. (E) Heatmap of the correlation between hub genes and representative pathway markers qualified by q-RT PCR. (F) Scatter plots of the correlation of hub genes and myocardial hypertrophy markers. (G) Correlation of the structure and function parameters of the left ventricle in mice and the expression of six hub genes. ANG II, angiotensin II. ESAWT, end-systolic anterior wall thickness. EDAWT, end diastolic anterior wall thickness. ESPWT, end systolic posterior wall thickness. EDPWT, end diastolic posterior wall thickness. EDD, end-diastolic dimension. ESD, end-systolic dimension. ESV, end-systolic volume. EDV, end-diastole volume. ***P < 0.001; **P < 0.01; *P < 0.05.
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Frontiers in Pharmacology
July 31 2025

Fig 2: circTNPO3 facilitated GEM chemoresistance through competitively binding miR-188-5p, upregulating CDCA3 and activing NF-κB nuclear translocation. (A) The binding sequence between miR-188-5p and CDCA3 mRNA was listed, and the direct binding potential was confirmed using dual-luciferase reporter assay. (B) The binding ability between miR-188-5p and CDCA3 mRNA was determined using miR-188-5p probe RNA pulldown assay. (C) The differential expression of CDCA3 between chemoresistant PC cells and parental non-chemoresistant PC cells was analyzed using qRT-PCR and immunoblotting assays. *P < 0.05, **P < 0.01 versus HPDE6-C7 group. #P < 0.05, ##P < 0.01 versus respective parental non-chemoresistant control (BxPC-3, CFPAC-1) group. (D) Pan-cancer atlas of CDCA3 in various human malignant tumors, and the differential expression of CDCA3 in PC was labeled by a red arrow. (E,F) The expression level of CDCA3 in PC was assessed by TCGA, GTEx and TNMplot databases. (G) Kaplan-Meier curve according to GEPIA database was used to analyze the overall survival of PC patients with lower and higher CDCA3 expression level. (H) The expression level of CDCA3 between PC tumor tissues and adjacent normal tissues was compared using IHC assay. (I) The correlation between miR-188-5p and CDCA3 in PC was analyzed through Pearson correlation coefficient. (J,K) The expression level of CDCA3 in BxPC-3/GR and CFPAC-1/GR cells after transfection with sh-circTNPO3-1 and miR-188-5p inhibitor was detected using qRT-PCR and immunoblotting assays. *P < 0.05, **P < 0.01 versus sh-NC group. #P < 0.05, ##P < 0.01 versus sh-circTNPO3-1 group. (L) The correlation between CDCA3 and TRAF2 in PC was analyzed through Pearson correlation coefficient from TNMplot database. (M,N) The expression level of CDCA3 in BxPC-3/GR and CFPAC-1/GR cells after transfection with sh-CDCA3-1/-2 was examined using immunoblotting assay. (O) The expression level of TRAF2 and the NF-κB-p65 nuclear translocation in BxPC-3 and BxPC-3/GR cells after CDCA3 knockdown were determined using immunoblotting assay. *P < 0.05 versus sh-NC group. ##P < 0.01 versus sh-CDCA3-2 group. (P) The NF-κB-p65 nuclear translocation in BxPC-3 and BxPC-3/GR cells after CDCA3 knockdown was evaluated using IF assay. IF, immunofluorescence.

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CDCA3 Polyclonal Antibody

The CDCA3 Polyclonal Antibody from Bioss Inc. is a Rabbit Polyclonal antibody to CDCA3. This antibody recognizes Rat antigen. The CDCA3 Polyclonal Antibody has been shown to work in the following applications: EIA, Immunoassay, ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry - fixed, Immunohistochemistry - frozen, and Western Blot.

Description

CDCA3 (Cell division cycle associated 3) is an F-box-like protein which is required for entry into mitosis. It is associated with SKP1 as part of a SCF (SKP1-cullin-F-box) protein ligase complex, and is required for the ubiquitination and degradation of the CDK1 inhibitory tyrosine kinase WEE1 at G2/M phase

Frontiers in Immunology

Frontiers in Pharmacology

CDCA3 Polyclonal Antibody from Bioss Inc.

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Bioss Inc.

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CDCA3 Polyclonal Antibody from Bioss Inc.

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