LEF1 (C12A5) Rabbit mAb 2230S from Cell Signaling Technology

Product Specs
ItemLEF1 (C12A5) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number2230S
Quantity100 µl
ApplicationsW, IF-IC, FC-FP
ReactivityH, M, R
TargetLEF1
Molecular Weight25-58 kDa
HostRabbit
NCBI Gene AliasesLEF-1, TCF10, TCF1ALPHA, TCF7L3
ClonalityMonoclonal
NCBI Full Gene Namelymphoid enhancer binding factor 1
CloneC12A5
Research AreaDevelopmental Biology

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Plasticity-Driven Regeneration of Circumvallate Papilla After Lgr5+ Stem Cells LossInt J Biol SciMay 11, 2026Senthil Kumar Baskaran, Anish Ashok Adpaikar, Suyeon Lee, Junsu Kim, Yeo Song Lee, JooHyun Jee, Hidenori Akutsu, Thantrira Porntaveetus, Ki Woo Kim, Eun-Jung Kim, et al.T369(AP0564, ABclonal, USA, 1:200), p70 S6 Kinase Antibody T421/S424 (AP0502, ABclonal, USA, 1:200), Lef1 (2230S, Cell Signaling Technology, USA, 1:200), Rock1 (ab45171, Abcam, UK, 1:200), Rock2 (610623, BD Bioscience,

(2) GPR124 regulates hyaloid blood vessel regression and is associated with endothelial-mesenchymal transitionSci RepApril 29, 2026Laura Hannig, Robin Heiden, Süleyman Ergün, Barbara M. Braunger, Mario Vallonanti-ZEB1, 1:100, Cell Signaling Technology #70512; rabbit anti-LEF1, 1:100, Cell Signaling Technology #2230; rabbit anti-VE-Cadherin, 1:500, Abcam ab205336; rabbit anti-Fibronectin, 1:100, Abcam ab199056) in

(3) Testosterone Exposure During Fetal Masculinization Programming Window Determines the Kidney Size in Adult MiceFASEB JApril 15, 2026Arttu Junnila, Kalle T. Rytkönen, Guillermo Martinez-Nieto, Mats Perk, Otto Mäkelä, Hao Li, Jenni Airaksinen, Ida Hyötyläinen, Oliver Mehtovuori, Asta Laiho, et al.,Thermo Scientific,RM‐9104‐S1,AB_149913,1:500,IF anti‐Jagged 1,Rat,Hybridoma Bank,TS1‐15H,AB_528317,1:300,IF anti‐LEF1,Rabbit,Cell Signaling Technology,2230,AB_823558,1:400,IF anti‐PAX2,Rabbit,Invitrogen,71–6000,AB_2533990,1:400,IF anti‐Calbindin,Goat,Santa Cruz Biotechnology

(4) RUNX1-deficiency drives immune-active ER + mammary tumorigenesis through activation of interferon signalingbioRxivApril 11, 2026Sen Han, Dongxi Xiang, Xueqing Chen, Dongyi Zhao, Guyu Qin, Roderick T. Bronson, Zhe LiBD Biosciences #610154, 1:100), LEF1 (C12A5, Rabbit Monoclonal Antibody, Cell Signaling Technology #2230T, 1:400), and Ki-67 (D3B5, Rabbit Monoclonal Antibody, Cell Signaling Technology #9129T, 1:400), diluted

(5) Testosterone Exposure During Fetal Masculinization Programming Window Determines the Kidney Size in Adult MiceFASEB JApril 4, 2026Arttu Junnila, Kalle T. Rytkönen, Guillermo Martinez‐Nieto, Mats Perk, Otto Mäkelä, Hao Li, Jenni Airaksinen, Ida Hyötyläinen, Oliver Mehtovuori, Asta Laiho, et al.,Thermo Scientific,RM‐9104‐S1,AB_149913,1:500,IF anti‐Jagged 1,Rat,Hybridoma Bank,TS1‐15H,AB_528317,1:300,IF anti‐LEF1,Rabbit,Cell Signaling Technology,2230,AB_823558,1:400,IF anti‐PAX2,Rabbit,Invitrogen,71–6000,AB_2533990,1:400,IF anti‐Calbindin,Goat,Santa Cruz Biotechnology

(6) A FZD4/LRP5 agonist restores pericyte coverage and vascular integrity by increasing PDGFB signalingbioRxivMarch 17, 2026Jacklyn Levey, Miranda Howe, Kaia Douglas, Emmanuel Odame, Neal Rajvansh, Ha-Neul Jo, Lingling Zhang, Christina Chung, Heidi Roehrich, Somasekar Seshagiri, et al.with 4 μg/ml puromycin. LEF1 was stained in PFA-fixed cells using rabbit anti-LEF1 (Cell Signaling, #2230S 1:100) and goat anti-rabbit Alexa Fluor-555 (Invitrogen, #A21428). Cells were stimulated for 24 hours

(7) β-catenin mutation reprograms ketone body metabolism to drive hepatocellular carcinoma metastasis and resistance to ketogenic therapy via transcriptional activation of OXCT1Cell Death & DiseaseMarch 9, 2026Huan Li, Liyuan Qian, Yifan Ji, Yuanhao Geng, Yanjun Lu, Laizhu Zhang, Yanchao Xu, Weiwei Zong, Xiang Jiang, Xianwei Zhou, et al.to the magnetic beads. The immunocomplex was subjected to immunoblotting using the LEF1 antibody (#2230, Cell Signaling Technology, USA), and corresponding secondary antibodies. Chromatin Immunoprecipitation

(8) PDZK1‐ULK1 Axis Triggers Lipophagy to Inhibit Tumor Progression and Sunitinib Resistance in Clear Cell Renal Cell CarcinomaAdvanced ScienceFebruary 16, 2026Xuan Qi, Yu Guo, Yumeng Yang, Haibo Wang, Xiaomei Yang, Ran Song, Qiong Qin, Yan Zhang, Meihan Hu, Haixing Zhou, et al.and Lamin B (#PTM‐6357) from PTM BIO (Hangzhou, China). MAP1LC3B (#3868), ULK1 (#8054), and LEF1 (#2230) from CST. Importin α5 (#18137) from Proteintech (Wuhan, China). 4.3 Co‐Immunoprecipitation (Co‐IP) Treated

(9) Rete ridges form via evolutionarily distinct mechanisms in mammalian skinNatureFebruary 4, 2026Sean M. Thompson, Violet S. Yaple, Gabriella H. Searle, Quan M. Phan, Jasson Makkar, Xiangzheng Cheng, Ruiqi Liu, Anna Pulawska-Czub, Corin Yanke, Natalie M. Williams, et al.lot no. 8136528, 3055226, 1214287, 1033227), human LEF1 rabbit (1:200, Cell Signaling, catalogue no. 2230S, clone C12A5, lot no. 8, 9), human-aSMA rabbit (1:1,000, Abcam, catalogue no. ab5694, clone proprietary,

(10) Stress-induced sympathetic hyperactivation drives hair follicle necrosis to trigger autoimmunityCellJanuary 8, 2026Emily Scott-Solomon, Shlomi Brielle, Alexander O. Mann, Mark J. Khoury, Jingyu Peng, Liana Tellez, Mackenzie Harrigan, Myrto Ziogas, H. Amalia Pasolli, Monica Cassandras, et al.,Thermo Fisher Scientific,Catalog# MA5–29706; RRID: AB_2785530 Rabbit anti Lef1 (1:150),Cell Signaling Technology,Catalog# 2230; RRID: AB_823558 Mouse anti SMA-Cy3 (1:300),MilliporeSigma,Catalog# C6198; RRID:AB_476856 Goat anti

(11) Targeting the tumor cell-intrinsic ITGB2 axis inhibits melanoma progressionMol CancerDecember 18, 2025Erik Rasbach, Laure Migayron, Anne Brandenburg, Praveen Singh, Christina Martins, Ali Kassem, Anastasia I. Karkempetzaki, Nadine Suessner, Zsofi Kulcsar, Jessica Riopedre, et al.AB_11127203), unconjugated rabbit anti-mouse LEF-1 ab (clone C12A5, Cell Signaling Technology, Cat# 2230, RRID: AB_823558), unconjugated rabbit anti-mouse p-VANGL2 (Thr78, Ser79, Ser82) ab (clone 7H7C2, Thermo Fisher Scientific

(12) The citrullinating enzyme PADI4 governs progenitor cell proliferation and translation in developing hair folliclesScience AdvancesSeptember 12, 2025Kim Vikhe Patil, Nil Campamà Sanz, Kylie Hin-Man Mak, Wei Yang, Karl Annusver, Jasson Makkar, David Grommisch, Li Lei, Priyanka Sharma, Ryan R. Driskell, et al.analysis. Antibodies used for IF K5 (BioLegend, 905901; 1:500), LEF1 (Cell Signaling Technology, 2230T; clone C12A5; 1:1000), GATA3 (Santa Cruz Biotechnology, sc-268 1:100), GATA3 (Abcam, ab110093; KT77;

(13) Accelerating Bone Healing With METTL3 Overexpressed Adipose‐Derived Stem Cells in Osteoporotic RatsCell ProliferationSeptember 1, 2025Hui Tang, Zhenzhen Chen, Lu Zeng, Yuping Xie, Daowen Luo, Shuanglin Peng, Fangzhi Lou, Tianli Wu, Jingang Xiao(ab92336), OPN (ab91655), β‐CATENIN (ab32572), GSK3β (ab32391), METTL3 (ab195352) (Abcam, UK) and LEF1 (2230p), P‐GSK‐3β (5558) (Cell Signalling Technology, USA). After 24 h, the membranes were rinsed thoroughly

(14) An evolutionarily conserved role for CTNNB1/β-CATENIN in regulating the development of the corpus callosumiScienceAugust 9, 2025Arpan Parichha, Debarpita Datta, Amrita Singh, Ishita Talwar, Shreya Yadav, Mahima Bose, Varun Suresh, Špela Miroševič, Nina Žakelj, David Gosar, et al.SOX9,Abcam,Cat# 185966; RRID: AB_2728660 Rabbit monoclonal anti LEF1,Cell Signaling Technology,Cat# 2230; RRID: AB_823558 Rabbit monoclonal anti-CALRETININ,ABclonal,Cat# A21965; N/A Rabbit monoclonal anti-TBR1,Invitrogen,Cat#

(15) The integrated stress response fine-tunes stem cell fate decisions upon serine deprivation and tissue injuryCell metabolismAugust 5, 2025Novak JSS, Polak L, Baksh SC, Barrows DW, Schernthanner M, Jackson BT, Thompson EAN, Gola A, Abdusselamoglu MD, Bonny AR, et al.anti-PHGDH antibody,Sigma,Cat# HPA021241 Rabbit monoclonal anti-LEF1 antibody,Cell Signaling,Cat# 2230S Rat monoclonal anti-GATA3 antibody,Invitrogen,Cat# 14-9966-82 Rat monoclonal anti-CD31 antibody,Biolegend,Cat#

(16) Sulforaphane-Rich Broccoli Sprout Extract Promotes Hair Regrowth in an Androgenetic Alopecia Mouse Model via Enhanced Dihydrotestosterone MetabolismInt J Mol SciAugust 1, 2025Laxman Subedi, Duc Dat Le, Eunbin Kim, Susmita Phuyal, Arjun Dhwoj Bamjan, Vinhquang Truong, Nam Ah Kim, Jung-Hyun Shim, Jong Bae Seo, Suk-Jung Oh, et al.anti-β-catenin (Cell Signaling Technology, #8480S, Danvers, MA, USA), anti-Lef-1 (Cell Signaling Technology, #2230S, Danvers, MA, USA), anti-Akr1c21 (Invitrogen, PA5-36572, Carlsbad, CA, USA), or anti-Dhrs9 (MyBioSource,

(17) Human adipose-derived mesenchymal stem cell-derived exosomes induce epithelial remodeling and anti-scar healing revealed by single-cell RNA sequencingJournal of nanobiotechnologyJuly 14, 2025Fu Y, Xie JL, Zhang XL, Xie GM, Zhang XM, Han YT, Xu MM, Zhang J, Zhang J.Waltham, MA; 00-5523-00). The cells were stained with LEF1 (C12A5) Rabbit (Cell Signaling Technology Cat# 2230, RRID: AB_823558), Brilliant Violet 421™ anti-mouse/human CD324 (BioLegend Cat# 147319, RRID: AB_2750483),

(18) The MDM2-p53 axis regulates norrin/frizzled4 signaling and blood-CNS barrier functionScience SignalingJuly 8, 2025Jacklyn Levey, Md. Abedin, Chi Zhang, Emmanuel Odame, Lingling Zhang, Ha-Neul Jo, Kaia Douglas, Heidi Roehrich, Zhe Chen, Harald J. Jungeperformed in blocking buffer at 4°C with shaking overnight using anti-Lef1 (1:100, Cell Signaling, 2230S), anti-Cldn5-Alexa488 (1:100, Invitrogen, 352588), anti-Mfsd2a (1:100, Cell Signaling, 80302S), or anti-Erg-Alexa647

(19) Platelet-rich plasma-derived exosomes stimulate hair follicle growth through activation of the Wnt/β-Catenin signaling pathwayRegenerative TherapyJune 1, 2025Changpei Lu, Yunbu Ding, Rongshuang Zhang, Yimei Du, Lingbo Bi, Min Zhao, Chaofan Wang, Qiaofang Wu, Haixia Jing, Weixin Fanprimary antibodies against β-Catenin (1:1000 dilution, CST, 8480, USA), Lef1 (1:1000 dilution, CST, 2230, USA), SFRP1 (1:3000 dilution, Proteintech, 26460-1-AP, Chicago, IL, USA), and β-actin (1:5000 dilution,

(20) An RNA transmethylation pathway governs kidney nephrogenic potentialNat CommunMay 28, 2025Harini Ramalingam, Jesus Alvarez, Andrea Flaten, Patricia Cobo-Stark, Nicholas Foster, Elyse Grilli, Ronak Lakhia, Karam Aboudehen, Thomas Carroll, Vishal Patel(Thermo Fisher, catalog# 610182), GFP (Thermo Fisher, catalog# NC9510598), Lef1 (Cell Signaling catalog# 2230S), Lhx1 (Developmental Studies Hybridoma Bank, catalog# 4F2), Lrpprc (Proteintech, catalog# 21175-1-AP),

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8/6/2014EMT Analysis: Lef1 Detection by WB Raul PeñaEpithelial to Mesenchimal Transcription (EMT) is a cellular process in which cells transform their phenotype into a more fibroblast-like one. Snail1 protein has been described as the critical player ... Read Review

LEF1 (C12A5) Rabbit mAb

LEF1 (C12A5) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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LEF1 (C12A5) Rabbit mAb from Cell Signaling Technology

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