Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb 4947S from Cell Signaling Technology

Product Specs
ItemPhospho-IRF-3 (Ser396) (4D4G) Rabbit mAb
CompanyCell Signaling Technology
Price $410.00 Inquire
Catalog Number4947S
Quantity100 µl
ApplicationsW
ReactivityH, M
TargetIRF-3 (Ser396) phosphate
ModificationsIRF-3 (Ser396) phosphate
HostRabbit
ClonalityMonoclonal
NCBI Full Gene Nameinterferon regulatory factor 3
Research AreaImmunology and Inflammation
Clone4D4G
NCBI Gene AliasesIIAE7
Molecular Weight45-55

Questions and Answers

Can this antibody be used to study the role of IRF-3 in the context of autoimmune diseases?

Yes, the Phospho-IRF-3 (Ser396) antibody has been utilized in studies investigating the cGAS-STING pathway, which is implicated in autoimmune diseases such as systemic lupus erythematosus. For instance, research has shown that dysregulation of STING signaling can lead to elevated type I interferon production, contributing to autoimmune pathology ().

What experimental techniques is this antibody suitable for?

The Phospho-IRF-3 (Ser396) antibody is suitable for Western Blot applications, as indicated by its use in various studies to detect phosphorylated IRF-3 levels in response to different stimuli, including viral infections and immune signaling pathways (, ).

Is this antibody effective in both human and mouse models?

Yes, the antibody has been shown to react with both human and mouse IRF-3, making it effective for studies across these species. This cross-reactivity is beneficial for comparative studies in immunology and inflammation research ().

How has this antibody been utilized in recent research?

Recent studies have employed the Phospho-IRF-3 (Ser396) antibody to investigate the mechanisms of innate immune responses, particularly in the context of STING signaling and its role in diseases like Niemann-Pick disease type C and lupus. The antibody has been cited in multiple publications, highlighting its relevance in understanding the phosphorylation status of IRF-3 during immune activation (, ).

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) The COX2-PGE2-PKA Axis Suppresses Antiviral Immunity by Inhibiting mtDNA-Dependent STING ActivationbioRxivApril 6, 2026Pham Thuy Tien Vo, Julien Cicero, Zichen Wang, Hiroyuki Hakozaki, Thomas S Hoang, Sendi Rafael Adame-Garcia, Dana J. Ramms, Kuniaki Sato, Erica Stevenson, Yuan Zhou, et al.(#5842), PKA RIa/b (#3927), GAPDH (#2118), STING (#13647), pTBK1 S172 (5483), TBK1 (#3504), pIRF3 S396 (#4947), IRF3 (#4302), TFAM (#8076), HSP90 (#4877), pUB S65 (#62802), COX4 (#4850), pPINK1 S228 (#89010), STOML2

(2) PIM1 Inhibition Alleviates Aspergillus fumigatus Keratitis by Regulating DDX41-Mediated STING Signaling PathwayInvest Ophthalmol Vis SciApril 1, 2026Fang Han, Leyi Wang, Jiayin Wu, Hanlin Ma, Xia Luo, Jianqiao LiSTING (Ser366, 19781; Ser365, 72971; 1:1000), phosphorylated TBK1 (5483, 1:1000), phosphorylated IRF3 (4947, 1:1000), and DDX41 (15076, 1:1000) were obtained from Cell Signaling Technology (Danvers, MA, USA).

(3) Short-chain acyl-CoA dehydrogenase initiates mtDNA demethylation and leakage to fuel antitumor immunity in colorectal cancerSignal Transduct Target TherMarch 26, 2026Fang Yang, Meng Wang, Shaofan Hu, Xu Guan, Kun Zhao, Yong Zhou, Hui Yao, Tianying Zhang, Liuli Li, Yuan Gao, et al.Invitrogen), STING (#A3575, ABclonal), p-TBK1 (#AP1026, ABclonal), TBK1 (#AF8103, Beyotime), p-IRF3 (#4947, Cell Signaling Technology), IRF3 (#4302, Cell Signaling Technology), BAX (#A0207, ABclonal), VDAC1

(4) STING signaling modulation by COPII cargo recognitionCellMarch 25, 2026Heng Lyu, Cong Xing, Wanwan Huai, Kun Song, Devon Jeltema, Hui Zhang, Xuewu Zhang, Nan Yan,Cell Signaling Technology,Cat#4302S; RRID: AB_1904036 anti-phospho-IRF3(S396),Cell Signaling Technology,Cat#4947S; RRID: AB_823547 anti-p65,Cell Signaling Technology,Cat#8242S; RRID: AB_10859369 anit-phospho-p65(S536),Cell Signaling Technology

(5) CRM1-dependent nuclear export of TRIM28 promotes MAVS K48-linked ubiquitination and suppresses RIG-I-mediated antiviral responseFrontiers in ImmunologyMarch 24, 2026Ruihua Xin, Wenshu Zou, Zhengying Qiu, Lina Huang, Xiaohan Jing, Qilin Huang, Zixiang Zhu, Jianxi Li, Mutien-Marie Gariglianyubiquitin (#12805S), MAVS (#24930S), TBK1 (#38066S), phospho-TBK1 (#5483S), IRF3 (#4302S), phospho-IRF3 (#4947S), HA (#3724S), and c-Myc (#2276S) were obtained from Cell Signaling Technology (CST, USA). The anti-GFP

(6) Induction of caspase-mediated suppression of interferon signaling potentiates viral oncolysis of resistant tumor cellsCell Communication and SignalingMarch 24, 2026Vladimir Bozhdansky, Shelly Tayar, Shlomi Kashkash, Eran Bacharach, Marcelo Ehrlich717–745; ABIN1881847, Rockland Immunochemicals Inc; 1:1000), rabbit anti-phospho-Ser396-IRF3 (cat. #4947; 1:1000), rabbit anti-IRF3 (cat. #4302; 1:1000),, rabbit anti-poly (ADP-ribose) polymerase 1(PARP1,

(7) Carnosic Acid Activates the STING/IRF3 Pathway to Induce Nitric Oxide-Mediated Apoptosis in Osteosarcoma CellsAntioxidants (Basel)March 16, 2026Weixiong Guo, Lanlan Yin, Qiang Wu, Jiaqi Chuanti-p-TBK1 (Ser172, CST, Cat# 5483), anti-IRF-3 (CST, Cat# 11904), anti-p-IRF-3 (Ser396, CST, Cat# 4947), anti-cleaved caspase-3 (CST, Cat# 9664), anti-PARP (CST, Cat# 9542), anti-Bax (CST, Cat# 5023), anti-Bcl-2

(8) Mycobacterium tuberculosis antigen-containing exosomes reinforce BCG vaccine efficacy by augmenting long-term protection and memory response against experimental tuberculosis in BALB-C miceFront ImmunolMarch 13, 2026Manu Sharma, Sher Afghan, Amit Singh, Iqbal Alam, Meetu Agarwal, Mohd Shahid, Shaikh Muhammad Atif, Saif Khan, Syed Saima Malik, Vengala Rao Yenuganti, et al.mouse monoclonal antibody (SC-23954, 1:1,000) were procured from Santa Cruz Biotechnology (USA). pIRF3 (4947, 1:1,000), pSTING (85735, 1:1,000), tubulin (2144, 1:5,000), HRP-conjugated anti-rabbit IgG (7074, 1:5,000),

(9) Ultrasound-triggered dissolving microneedle platform for cGAS-STING-mediated precision immunotherapy of melanomaMater Today BioMarch 2, 2026Juan Wang, Siqi Yuan, Xiuwen Zhang, Yanyan Zhang, Mengting Zhang, Jing Chen, Yan Shen, Yu Tianfrom Biolegend (San Diego, CA, USA). Phospho-STING (72971), Phospho-TBK1/NAK (5483), Phospho-IRF-3 (4947), and Phospho-Histone H2A.X (9718) were purchased from Cell signaling Technology. Alexa 488-conjugated

(10) PCBP2 inhibits antiviral innate immune responses via the MAVS-mediated signaling pathway in severe fever with thrombocytopenia syndromeVirus ResearchMarch 1, 2026Xinyi Yu, Yan Dai, Xuewen Ji, Qinqin Pu, Ruonan Zhang, Mengqi Shi, Nannan Hu, Ke Jin, Jin Zhu, Jun Lianalysis. 2.12 Antibodies β-Actin (13E5) rabbit mAb (Cat# 4970, 1:1000), rabbit anti-phospho-IRF3 (Cat# 4947, 1:1000), rabbit anti-IRF3 (Cat# 4302, 1:1000), rabbit anti-phospho-TBK1 (Cat# 5483, 1:1000), HA-Tag (C29F4) Rabbit mAb

(11) PROTAC-based synthetic lethality strategy endogenously activates systemic STING to boost antitumor immunityScience AdvancesFebruary 27, 2026Ye Liu, Maolin Jiang, Mengchao Ding, Ihsan Ullah, Haiyang Wang, Wenjiao Xie, Kewei Wang, Youyong Yuan(1:1000; CST, no. 15102), p-STING (Ser366: 1:500; CST, no. 72971), and p-IRF3 (Ser396: 1:500; CST, no. 4947). Immunofluorescence staining Degradation of BRD4 and PARP1 was confirmed through confocal laser scanning microscopy

(12) Epigenetic control of microglial mitochondrial immunity by KAT7 drives Alzheimer’s disease pathogenesisbioRxivFebruary 24, 2026Yongqing Liu, Minghua Fan, Yingzhi Ye, Henry Yi Cheng, Shuying Sun, Zhaozhu Qiu1:100, Cell Signaling Technology, #5483), anti-phospho-IRF3 (rabbit, 1:100, Cell Signaling Technology, #4947), anti-GFAP (mouse, 1:400, Invitrogen, 149892-82), anti-NeuN (mouse, 1:200, Millipore, MAB377). Preparation

(13) cGAS/STING-mediated upregulation of NKG2D ligands in LSCs contributes to enhanced sensitivity to NK cellsFrontiers in OncologyFebruary 23, 2026Yan Ouyang, Yilin Qin, Qianmin Zhang, Pengcheng Zhu, Huifang ZhuCell signaling technology), IRF3(Cat#: 4302, Cell signaling technology), phospho-IRF3 (Ser396) (Cat#: 4947, Cell signaling technology), ATM (Cat#: 27156-1-AP, Proteintech), phospho-ATM (Ser1981) (Cat#: 5883,

(14) C-terminal tail of MAVS dictates organelle targeting and innate immune responseCell Communication and SignalingFebruary 21, 2026Terence Tak-Wang Lee, Kiki Cham, Dong-Yan JinTechnology,#4302 Rabbit monoclonal anti-Phospho-IRF-3 (Ser396) (IB: 1000),Cell Signalling Technology,#4947 Mouse monoclonal anti-TOM20 (IF: 1:100),Santa Cruz,sc-17764 PMP70 (IF: 100),Sigma-Aldrich,SAB4200181 Drugs,, G418

(15) RNF20 dynamically regulates RIG-I and MDA5 transcription and degradation via nucleocytoplasmic translocation to balance antiviral signalingPLOS PathogensFebruary 19, 2026Jie Wang, Qiuju Liu, Shurui Zhao, Qi Shao, Feiyu Fu, Kehui Zhang, Jingjiao Ma, Zhaofei Wang, Yaxian Yan, Hengan Wang, et al.(Cell Signaling Technologies, 5483), anti-IRF3 (Cell Signaling Technologies, 11904), anti-p-IRF3 (Cell Signaling Technologies, 4947), anti-STING (Cell Signaling Technologies, 13647S), anti-STAT1 (Sangon Biotech, D120084). Cell treatment To

(16) ERRα-KDM5C restrains STING enhancer activity to modulate type I interferon signaling in breast cancer progressionCell Death & DiseaseFebruary 18, 2026Zu-Hui Xu, Jie Chen, Ying He, Cheng Lei, Xiao-Ling Wang, De-Fa Huang, Zheng-Zhe Li, Hui Zhou, Mei-Yan Wang, Cheng-Gui Song, et al.anti-TBK1 (Cell Signaling Technology, 3504, 1:1000), Rabbit anti-Phospho-IRF3 (Cell Signaling Technology, 4947, 1:1000), Rabbit anti-Phospho-IRF3 (Cell Signaling Technology, 4302, 1:1000), Mouse anti-HA (ABclonal,

(17) Investigating the impact and mechanism of Licochalcone B derivative CTG12 on NLRP3 inflammasomeCell Communication and SignalingFebruary 18, 2026Shuyi Bian, Zhi-E Fang, Lichen Wang, Jiayi Wang, Ming Dong, Nan Yang, Jiabo Wang, Ling Li, Guang Xu(G1780) were procured from Promega (USA). Anti-human IL-1β (p17, 12242S), anti-Phospho-IRF3 (Ser396) (4947), caspase-1 antibody (4199S) and anti-ASC antibody (sc-22, 514-R) were obtained from Cell Signaling Technology

(18) Dissection of innate-immune-ligand- and interferon-protein-mediated transcriptional responses in human THP1 cell statesCommun BiolFebruary 12, 2026Lodoe Lama, Pavel Morozov, Aitor Garzia, Thomas TuschlIRF7 4920S, IRF8 5628S, IRF9 76684S, STAT1 9175S, pSTAT1-Y701 9167S, pIRF3-S386 37829S, pIRF3-S396 4947S, pTBK1-S172 5483S, pSTING-S366 19781S, NF-κB-RELA 8242S, NF-κB-pRELA-S536 3033S; R&D Systems, IRF2 AF4049;

(19) STING activation induces cytotoxic and immune responses in meningiomas via inflammatory cell death pathwaysNature CommunicationsFebruary 12, 2026Mark W. Youngblood, Shashwat Tripathi, Hinda Najem, Harrshavasan Congivaram, Mateo Gomez, Thomas K. Sears, Lisa Hurley, Leah K. Billingham, Caylee Silvers, Wenxia Wang, et al.cat#13647), and p-IRF3 (downstream activation of the STING pathway; Cell signaling, clone 4D4G, cat#4947). All antibodies were validated using conventional immunohistochemistry and/or immunofluorescence (IF) staining

(20) A sex-biased response of the adult brain to Zika virus infection: from down-regulation of neuronal IRF3 to enhanced pathology in adult female miceCell Communication and SignalingFebruary 11, 2026Zeyni Mansuroglu, Violaine Bortolin, Fanny Lambert, Laurine Conquet, Ignacio Garcia-Verdugo, Gaetano Calcagno, Marie Bourdon, Sylvie Souès, Florence Niedergang, Caroline Manet, et al.antibodies for Western blot (WB): anti-IRF3 (Cell Signaling, 4302 S); anti-phIRF3 (Cell Signaling, 4947 S); anti-NS2B (Genetex GTX13308); anti-GAPDH (Cell Signaling, 97166). Primary antibodies for immunofluorescence:

Write a Review

(1)

2/2/2024Phospho-IRF Antibody From Cell Signaling Technology Works Well for Western Blot Gherardo BaudoIn the SH-SY5Y cell study with beta-amyloid or isolated mitochondria treatment, the pIRF-3 antibody (#4947S) consistently exhibited reliability and specificity. At a 1:1000 dilution in 5% BSA TBST, it... Read Review
(1)

7/30/2015Review for Rabbit Anti–Phospho-IRF3 Antibody Liyong Z.Protein extract from cells was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied with PMSF, protease inhibitor mixture, sodium orthovanadate, or ... Read Review
(1)

10/31/2013IRF3 Activation Measurement During Viral Infections F. BiotaThe goal of this experiment was to compare antiviral signalling between two sets of MEFS (WT vs KO). MEFs were infected with Sendai virus and antiviral signalling was monitored by measuring the ... Read Review

Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb

Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb from Cell Signaling Technology

Description

Questions and Answers

Cell Signaling Technology

Citations (681)

Reviews

Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map